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BNP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751315

Description: Rabbit IgG polyclonal antibody for BNP detection. Tested with WB, Direct ELISA in Human; Mouse.Background: Brain natriuretic peptide (BNP), also known as B-type natriuretic peptide, is a member of the natriuretic peptide family and encodes a… More details

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Product Overview

Product Name
BNP/NPPB, Polyclonal Antibody
Full Product Name
Anti-BNP/NPPB Antibody
Product Gene Name
anti-BNP antibody
Product Synonym Gene Name
NPPB
Product Synonym Names
Natriuretic peptides B; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; BNP (1-32); BNP-32; BNP (1-30); BNP (1-29); BNP (1-28); BNP (2-31); BNP (3-32); BNP (3-30); BNP (3-29); BNP (4-32); BNP (4-31); BNP (4-30); BNP (4-29); BNP (4-27); BNP (5-32); BNP (5-31); BNP (5-29); NPPB; Natriuretic peptide B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
14,726 Da
3D Structure
ModBase 3D Structure for P16860
Immunogen
E Coli-derived human BNP recombinant protein (Position: H27-H134).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BNP antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes for anti-BNP antibody
WB: 0.1-0.5 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Western Blot (WB) of anti-BNP antibody

Figure 1. Western blot analysis of BNP using anti-BNP antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNP antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BNP at approximately 26KD. The expected band size for BNP is at 26KD.
Rabbit Anti-BNP Polyclonal Antibody – Cat# MBS1751315

Product References and Citations for anti-BNP antibody

1. Arden, K. C., Viars, C. S., Weiss, S., Argentin, S., Nemer, M. Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics 26: 385-389, 1995. 2. Marchini, A., Hacker, B., Marttila, T., Hesse, V., Emons, J., Weiss, B., Karperien, M., Rappold, G. BNP is a transcriptional target of the short stature homeobox gene SHOX. Hum. Molec. Genet. 16: 3081-3087, 2007.

Other Notes

Small volumes of anti-BNP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BNP purchase

MBS1751315 is an antibody targeting BNP. Also known as Anti-BNP/NPPB Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BNP. It may not necessarily be applicable to this product.

NCBI GI #
4505433
NCBI GeneID
4879
NCBI Accession #
NP_002512.1
UniProt Primary Accession #
P16860
UniProt Secondary Accession #
Q6FGY0; Q9P2Q7; B0ZBE9
UniProt Related Accession #
P16860
GenBank Nucleotide #
NM_002521.3
NCBI Official Full Name
natriuretic peptides B preproprotein
NCBI Official Synonym Full Names
natriuretic peptide B
NCBI Official Symbol
NPPB
NCBI Official Synonym Symbols
BNP
UniProt Protein Name
Natriuretic peptides B
UniProt Synonym Protein Names
Gamma-brain natriuretic peptide
UniProt Gene Name
NPPB
UniProt Synonym Gene Names
BNP(1-32); BNP-32
NCBI Protein Information
natriuretic peptides B

NCBI Summary for BNP

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
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UniProt Comments for BNP

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
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Research Articles on BNP

1. Patients diagnosed with portopulmonary hypertension (POPH) had significantly higher BNP levels in comparison with those patients without. In contrast, PELD/MELD scores showed an almost negligible correlation with the BNP level. Liver transplantation was successful in 3 asymptomatic biliary atresia patients with POPH who had high BNP levels despite the low PELD/MELD scores.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BNP antibody

 Reference Product  PubMed Publications
 NPPC antibody  >6 publications with BNP and NPPC
 NPR1 antibody  >5 publications with BNP and NPR1
 NPR3 antibody  >5 publications with BNP and NPR3
 MYH6 antibody  >4 publications with BNP and MYH6
 NPR2 antibody  >4 publications with BNP and NPR2
 TNNI3 antibody  >2 publications with BNP and TNNI3
 PLN antibody  >1 publications with BNP and PLN
 TNNT2 antibody  >1 publications with BNP and TNNT2
 IDE antibody  >1 publications with BNP and IDE

Pathways associated with anti-BNP antibody

 Products by Pathway  Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram

Diseases associated with anti-BNP antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >138 publications with BNP and Fibrosis
 Cardiovascular Diseases Antibodies  >72 publications with BNP and Cardiovascular Diseases
 Heart Diseases Antibodies  >36 publications with BNP and Heart Diseases
 Hypertension Antibodies  >26 publications with BNP and Hypertension
 Hypertrophy Antibodies  >18 publications with BNP and Hypertrophy
 Edema Antibodies  >15 publications with BNP and Edema
 Heart Failure Antibodies  >15 publications with BNP and Heart Failure
 Inflammation Antibodies  >14 publications with BNP and Inflammation
 Necrosis Antibodies  >13 publications with BNP and Necrosis
 Postoperative Complications Antibodies  >12 publications with BNP and Postoperative Complications

Organs/Tissues associated with anti-BNP antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >145 publications with BNP and Kidney
 Heart Antibodies  >84 publications with BNP and Heart
 Lung Antibodies  >60 publications with BNP and Lung
 Liver Antibodies  >44 publications with BNP and Liver
 Pancreas Antibodies  >29 publications with BNP and Pancreas
 Embryonic Tissue Antibodies  >15 publications with BNP and Embryonic Tissue
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