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GOLPH3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751336

Description: Rabbit IgG polyclonal antibody for GOLPH3 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a… More details

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Product Overview

Online Datasheet in PDF
Product Name
GOLPH3, Polyclonal Antibody
Full Product Name
Anti-GOLPH3 Antibody
Product Gene Name
anti-GOLPH3 antibody
Product Synonym Names
Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS; GOLPH3; GPP34
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
33,811 Da
3D Structure
ModBase 3D Structure for Q9H4A6
Immunogen
E Coli-derived human GOLPH3 recombinant protein (Position: K56-K298).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-GOLPH3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-GOLPH3 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-GOLPH3 antibody

Figure 1. Western blot analysis of GOLPH3 using anti-GOLPH3 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human placenta tissue lysates,
Lane 3: human U20S whole cell lysate,
Lane 4: human Caco-2 whole cell lysate,
Lane 5: human SW620 whole cell lysate,
Lane 6: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH3 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GOLPH3 at approximately 34KD. The expected band size for GOLPH3 is at 34KD.
Rabbit Anti-GOLPH3 Polyclonal Antibody – Cat# MBS1751336

Immunohistochemistry (IHC) of anti-GOLPH3 antibody

Figure 3. IHC analysis of GOLPH3 using anti-GOLPH3 antibody.
GOLPH3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLPH3 antibody.
GOLPH3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLPH3 antibody.
GOLPH3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLPH3 antibody.
GOLPH3 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLPH3 antibody.
GOLPH3 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLPH3 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-GOLPH3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751336

Product References and Citations for anti-GOLPH3 antibody

1. Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N. M., Choudhary, J. S., Lewis, A. P., Chotai, D., Fazel, A., Gushue, J. N., Paiement, J., Palcy, S., Chevet, E., Lafreniere-Roula, M., Solari, R., Thomas, D. Y., Rowley, A., Bergeron, J. J. M. Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem. 276: 5152-5165, 2001. 2. Ng, M. M., Dippold, H. C., Buschman, M. D., Noakes, C. J., Field, S. J.GOLPH3L antagonizes GOLPH3 to determine Golgi morphology. Molec. Biol. Cell 24: 796-808, 2013.

Other Notes

Small volumes of anti-GOLPH3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOLPH3 purchase

MBS1751336 is an antibody targeting GOLPH3. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLPH3. It may not necessarily be applicable to this product.

NCBI GI #
11545859
NCBI GeneID
64083
NCBI Accession #
NP_071413.1
UniProt Primary Accession #
Q9H4A6
UniProt Secondary Accession #
Q9UIW5
UniProt Related Accession #
Q9H4A6
GenBank Nucleotide #
NM_022130.4
NCBI Official Full Name
Golgi phosphoprotein 3
NCBI Official Synonym Full Names
golgi phosphoprotein 3
NCBI Official Symbol
GOLPH3
NCBI Official Synonym Symbols
GOPP1; GPP34; MIDAS; Vps74
UniProt Protein Name
Golgi phosphoprotein 3
UniProt Synonym Protein Names
Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS
Protein Family
Golgi phosphoprotein
UniProt Gene Name
GOLPH3
UniProt Synonym Gene Names
GPP34; MIDAS
NCBI Protein Information
Golgi phosphoprotein 3

NCBI Summary for GOLPH3

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for GOLPH3

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
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Research Articles on GOLPH3

1. Overexpression of GOLPH3 may be involved in the resistance of HT29 colon cancer cells to cisplatin chemotherapy by activating multiple cell signaling pathways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GOLPH3 antibody

 Reference Product  PubMed Publications
 MYO18A antibody  >4 publications with GOLPH3 and MYO18A
 VPS35 antibody  >1 publications with GOLPH3 and VPS35

Pathways associated with anti-GOLPH3 antibody

 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-GOLPH3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >15 publications with GOLPH3 and Neoplasms
 Liver Diseases Antibodies  >2 publications with GOLPH3 and Liver Diseases
 Genital Diseases, Male Antibodies  >2 publications with GOLPH3 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >2 publications with GOLPH3 and Prostatic Diseases
 Learning Disorders Antibodies  >1 publications with GOLPH3 and Learning Disorders
 Hyperplasia Antibodies  >1 publications with GOLPH3 and Hyperplasia

Organs/Tissues associated with anti-GOLPH3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with GOLPH3 and Lung
 Brain Antibodies  >4 publications with GOLPH3 and Brain
 Muscle Antibodies  >3 publications with GOLPH3 and Muscle
 Prostate Antibodies  >3 publications with GOLPH3 and Prostate
 Blood Antibodies  >2 publications with GOLPH3 and Blood
 Lymph Antibodies  >2 publications with GOLPH3 and Lymph
 Lymph Node Antibodies  >2 publications with GOLPH3 and Lymph Node
 Bone Antibodies  >1 publications with GOLPH3 and Bone
 Stomach Antibodies  >1 publications with GOLPH3 and Stomach
 Vascular Antibodies  >1 publications with GOLPH3 and Vascular
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