Rabbit Anti-CACNA2D2 Polyclonal Antibody – Cat# MBS1751357

Figure 1. Western blot analysis of CACNA2D2 using anti-CACNA2D2 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D2 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CACNA2D2 at approximately 130KD. The expected band size for CACNA2D2 is at 130KD.

CACNA2D2 Antibody – Rabbit Polyclonal

Name: CACNA2D2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1751357
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751357

Description: Rabbit IgG polyclonal antibody for CACNA2D2 detection. Tested with WB, IHC-F, ICC, FCM, Direct ELISA in Human; Mouse; Rat.Background: Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CACNA2D2, Polyclonal Antibody

Full Product Name

Anti-CACNA2D2 Antibody

Product Gene Name

anti-CACNA2D2 antibody

Product Synonym Names

Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2; Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2; CACNA2D2; KIAA0558; Calcium voltage-gated channel auxiliary subunit alpha2delta 2

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.

Molecular Weight

129,085 Da

3D Structure

ModBase 3D Structure for Q9NY47

Immunogen

E Coli-derived human CACNA2D2 recombinant protein (Position: Q66-Q284).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Relevant Detection Systems

It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-F and ICC.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CACNA2D2 antibody

Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Application Notes for anti-CACNA2D2 antibody

WB: 0.1-0.5 mug/ml
IHC-F: 0.5-1 mug/ml
ICC: 0.5-1 mug/ml
FC/FACS: 1-3ug/1x106 cells
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-CACNA2D2 antibody

1. Brill, J., Klocke, R., Paul, D., Boison, D., Gouder, N., Klugbauer, N., Hofmann, F., Becker, C.-M., Becker, K.Entla, a novel epileptic and ataxic Cacna2d2 mutant of the mouse. J. Biol. Chem. 279: 7322-7330, 2004. 2. Brodbeck, J., Davies, A., Courtney, J.-M., Meir, A., Balaguero, N., Canti, C., Moss, F. J., Page, K. M., Pratt, W. S., Hunt, S. P., Barclay, J., Rees, M., Dolphin, A. C. The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha-2/delta-2 protein with abnormal function. J. Biol. Chem. 277: 7684-7693, 2002.

Other Notes

Small volumes of anti-CACNA2D2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CACNA2D2 purchase

MBS1751357 is an antibody targeting CACNA2D2. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CACNA2D2. It may not necessarily be applicable to this product.

NCBI GI #

54112392

NCBI GeneID

9254

NCBI Accession #

NP_001005505.1

UniProt Primary Accession #

Q9NY47

UniProt Secondary Accession #

Q9NY48; Q9UEW0; Q9Y268; A7MD15

UniProt Related Accession #

Q9NY47

GenBank Nucleotide #

NM_001005505.2

NCBI Official Full Name

voltage-dependent calcium channel subunit alpha-2/delta-2 isoform a

NCBI Official Synonym Full Names

calcium voltage-gated channel auxiliary subunit alpha2delta 2

NCBI Official Symbol

CACNA2D2

NCBI Official Synonym Symbols

CACNA2D

UniProt Protein Name

Voltage-dependent calcium channel subunit alpha-2/delta-2

UniProt Synonym Protein Names

Voltage-gated calcium channel subunit alpha-2/delta-2

Protein Family

Voltage-dependent calcium channel

UniProt Gene Name

CACNA2D2

UniProt Synonym Gene Names

KIAA0558

NCBI Protein Information

voltage-dependent calcium channel subunit alpha-2/delta-2

NCBI Summary for CACNA2D2

Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

UniProt Comments for CACNA2D2

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis.

Research Articles on CACNA2D2

1. show that the action of alpha2delta2 on tumor development occurs not only through a stimulation of proliferation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CACNA2D2 antibody

Products by Pathway	Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies	Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Depolarization Of The Presynaptic Terminal Triggers The Opening Of Calcium Channels Pathway antibodies	Depolarization Of The Presynaptic Terminal Triggers The Opening Of Calcium Channels Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Inhibition Of Insulin Secretion By Adrenaline/Noradrenaline Pathway antibodies	Inhibition Of Insulin Secretion By Adrenaline/Noradrenaline Pathway Diagram

Diseases associated with anti-CACNA2D2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>15 publications with CACNA2D2 and Neoplasms
Epilepsy Antibodies	>9 publications with CACNA2D2 and Epilepsy
Lung Neoplasms Antibodies	>9 publications with CACNA2D2 and Lung Neoplasms
Seizures Antibodies	>7 publications with CACNA2D2 and Seizures
Brain Diseases Antibodies	>6 publications with CACNA2D2 and Brain Diseases
Epilepsy, Absence Antibodies	>6 publications with CACNA2D2 and Epilepsy, Absence
Neurologic Manifestations Antibodies	>5 publications with CACNA2D2 and Neurologic Manifestations
Ataxia Antibodies	>5 publications with CACNA2D2 and Ataxia
Pain Antibodies	>3 publications with CACNA2D2 and Pain
Kidney Neoplasms Antibodies	>2 publications with CACNA2D2 and Kidney Neoplasms