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Cardiac FABP/FABP3 (FABP3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751376

Description: Rabbit IgG polyclonal antibody for Cardiac FABP detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cardiac FABP/FABP3, Polyclonal Antibody
Full Product Name
Anti-Cardiac FABP/FABP3 Antibody
Product Gene Name
anti-FABP3 antibody
Product Synonym Names
Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; M-FABP; FABP3; FABP11; MDGI; Fatty acid binding protein 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
14,858 Da
3D Structure
ModBase 3D Structure for P05413
Immunogen
E Coli-derived human Cardiac FABP recombinant protein (Position: V2-A133).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FABP3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-FABP3 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-FABP3 antibody

Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.
Rabbit Anti-FABP3 Polyclonal Antibody – Cat# MBS1751376

Immunohistochemistry (IHC) of anti-FABP3 antibody

Figure 3. IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody.
Cardiac FABP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cardiac FABP antibody.
Cardiac FABP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cardiac FABP antibody.
Cardiac FABP was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Cardiac FABP antibody.
Cardiac FABP was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Cardiac FABP Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-FABP3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751376

Product References and Citations for anti-FABP3 antibody

1. "Entrez Gene: FABP3 fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor)". 2. Borchers, T., Hojrup, P., Nielsen, S. U., Roepstorff, P., Spener, F., Knudsen, J. Revision of the amino acid sequence of human heart fatty acid binding protein. Molec. Cell. Biochem. 98: 127-133, 1990. 3. Phelan CM, Larsson C, Baird S, Futreal PA, Ruttledge MH, Morgan K, Tonin P, Hung H, Korneluk RG, Pollak MN, Narod SA (Dec 1996). "The human mammary-derived growth inhibitor (MDGI) gene: genomic structure and mutation analysis in human breast tumors". Genomics 34 (1): 63-8.

Other Notes

Small volumes of anti-FABP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FABP3 purchase

MBS1751376 is an antibody targeting FABP3. Also known as Anti-Cardiac FABP/FABP3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FABP3. It may not necessarily be applicable to this product.

NCBI GI #
4758328
NCBI GeneID
2170
NCBI Accession #
NP_004093.1
UniProt Primary Accession #
P05413
UniProt Secondary Accession #
Q5VV93; Q99957; B2RAB6
UniProt Related Accession #
P05413
GenBank Nucleotide #
NM_004102.4
NCBI Official Full Name
fatty acid-binding protein, heart isoform 2
NCBI Official Synonym Full Names
fatty acid binding protein 3
NCBI Official Symbol
FABP3
NCBI Official Synonym Symbols
MDGI; FABP11; H-FABP; M-FABP; O-FABP
UniProt Protein Name
Fatty acid-binding protein, heart
UniProt Synonym Protein Names
Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; M-FABP
Protein Family
Fatty acid-binding protein
UniProt Gene Name
FABP3
UniProt Synonym Gene Names
FABP11; MDGI; H-FABP; MDGI; M-FABP
NCBI Protein Information
fatty acid-binding protein, heart

NCBI Summary for FABP3

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
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UniProt Comments for FABP3

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Research Articles on FABP3

1. the frequency of h-FABP positivity among acute myocardial infarction patients was higher than that of hs-TnI, which would have missed six of them; however, hs-TnI area under curve was superior to that of h-FABP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FABP3 antibody

 Reference Product  PubMed Publications
 FABP1 antibody  >17 publications with FABP3 and FABP1
 FAAH antibody  >2 publications with FABP3 and FAAH
 SIRT1 antibody  >1 publications with FABP3 and SIRT1

Pathways associated with anti-FABP3 antibody

 Products by Pathway  Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram

Diseases associated with anti-FABP3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >150 publications with FABP3 and Cardiovascular Diseases
 Heart Diseases Antibodies  >129 publications with FABP3 and Heart Diseases
 Nervous System Diseases Antibodies  >38 publications with FABP3 and Nervous System Diseases
 Fatty Liver Antibodies  >33 publications with FABP3 and Fatty Liver
 Necrosis Antibodies  >29 publications with FABP3 and Necrosis
 Heart Failure Antibodies  >24 publications with FABP3 and Heart Failure
 Inflammation Antibodies  >20 publications with FABP3 and Inflammation
 Disease Models, Animal Antibodies  >17 publications with FABP3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >15 publications with FABP3 and Breast Neoplasms
 Hypertension Antibodies  >11 publications with FABP3 and Hypertension

Organs/Tissues associated with anti-FABP3 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >236 publications with FABP3 and Heart
 Muscle Antibodies  >111 publications with FABP3 and Muscle
 Brain Antibodies  >82 publications with FABP3 and Brain
 Liver Antibodies  >34 publications with FABP3 and Liver
 Kidney Antibodies  >22 publications with FABP3 and Kidney
 Connective Tissue Antibodies  >22 publications with FABP3 and Connective Tissue
 Embryonic Tissue Antibodies  >13 publications with FABP3 and Embryonic Tissue
 Lung Antibodies  >12 publications with FABP3 and Lung
 Placenta Antibodies  >8 publications with FABP3 and Placenta
 Bone Antibodies  >5 publications with FABP3 and Bone
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