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INPPL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751385

Description: Rabbit IgG polyclonal antibody for INPPL1 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: SH2-domain containing Phosphatidylinositol-3, 4, 5-trisphosphate 5-phosphatase 2 is an enzyme that in humans is encoded by… More details

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Product Overview

Online Datasheet in PDF
Product Name
INPPL1, Polyclonal Antibody
Full Product Name
Anti-INPPL1 Antibody
Product Gene Name
anti-INPPL1 antibody
Product Synonym Names
Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 2; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Protein 51C; SH2 domain-containing inositol 5'-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2; INPPL1; SHIP2; Inositol polyphosphate phosphatase-like 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
113,176 Da
3D Structure
ModBase 3D Structure for O15357
Immunogen
E Coli-derived human INPPL1 recombinant protein (Position: R1172-K1258).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-INPPL1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-INPPL1 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-INPPL1 antibody

Figure 1. Western blot analysis of INPPL1 using anti-INPPL1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysate,
Lane 3: human U20S whole cell lysate,
Lane 4: human PC-3 whole cell lysate,
Lane 5: human Caco-2 whole cell lysate,
Lane 6: human A549 whole cell lysate,
Lane 7: human K562 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPPL1 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 140-160KD. The expected band size for INPPL1 is at 138KD.
Rabbit Anti-INPPL1 Polyclonal Antibody – Cat# MBS1751385

Immunohistochemistry (IHC) of anti-INPPL1 antibody

Figure 3. IHC analysis of INPPL1 using anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.
INPPL1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-INPPL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751385

Product References and Citations for anti-INPPL1 antibody

1. Habib, T., Hejna, J. A., Moses, R. E., Decker, S. J. Growth factors and insulin stimulate tyrosine phosphorylation of the 51C/SHIP2 protein. J. Biol. Chem. 273: 18605-18609, 1998. 2. Sleeman, M. W., Wortley, K. E., Lai, K.-M. V., Gowen, L. C., Kintner, J., Kline, W. O., Garcia, K., Stitt, T. N., Yancopoulos, G. D., Wiegand, S. J., Glass, D. J. Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity. Nature Med. 11: 199-205, 2005. 3. Yu, J., Ryan, D. G., Getsios, S., Oliveira-Fernandes, M., Fatima, A., Lavker, R. M. MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia. Proc. Nat. Acad. Sci. 105: 19300-19305, 2008.

Other Notes

Small volumes of anti-INPPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for INPPL1 purchase

MBS1751385 is an antibody targeting INPPL1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for INPPL1. It may not necessarily be applicable to this product.

NCBI GI #
222136583
NCBI GeneID
3636
NCBI Accession #
NP_001558.3
UniProt Primary Accession #
O15357
UniProt Secondary Accession #
Q13577; Q13578; B2RTX5
UniProt Related Accession #
O15357
GenBank Nucleotide #
NM_001567.4
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
NCBI Official Synonym Full Names
inositol polyphosphate phosphatase like 1
NCBI Official Symbol
INPPL1
NCBI Official Synonym Symbols
OPSMD; SHIP2
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
UniProt Synonym Protein Names
Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Protein 51C; SH2 domain-containing inositol 5'-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2
Protein Family
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
UniProt Gene Name
INPPL1
UniProt Synonym Gene Names
SHIP2; INPPL-1; SH2 domain-containing inositol phosphatase 2; SHIP-2
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2

NCBI Summary for INPPL1

The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer. [provided by RefSeq, Jan 2009]
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UniProt Comments for INPPL1

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling (PubMed:21624956). Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification.
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Research Articles on INPPL1

1. Results find SHIP2 expression negatively regulated by ZIC2. Its expression is downregulated in osteosarcoma cells and tissues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-INPPL1 antibody

 Reference Product  PubMed Publications
 PTEN antibody  >34 publications with INPPL1 and PTEN
 SHC1 antibody  >27 publications with INPPL1 and SHC1
 CBL antibody  >11 publications with INPPL1 and CBL
 MET antibody  >4 publications with INPPL1 and MET
 EPHA2 antibody  >3 publications with INPPL1 and EPHA2
 INPP4B antibody  >1 publications with INPPL1 and INPP4B
 ITSN1 antibody  >1 publications with INPPL1 and ITSN1

Pathways associated with anti-INPPL1 antibody

 Products by Pathway  Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway antibodies  1D-myo-inositol Hexakisphosphate Biosynthesis II (mammalian) Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 3-phosphoinositide Degradation Pathway antibodies  3-phosphoinositide Degradation Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,3,4)-trisphosphate Biosynthesis Pathway Diagram

Diseases associated with anti-INPPL1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >36 publications with INPPL1 and Neoplasms
 Immune System Diseases Antibodies  >26 publications with INPPL1 and Immune System Diseases
 Diabetes Mellitus, Type 2 Antibodies  >13 publications with INPPL1 and Diabetes Mellitus, Type 2
 Inflammation Antibodies  >9 publications with INPPL1 and Inflammation
 Cardiovascular Diseases Antibodies  >8 publications with INPPL1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >6 publications with INPPL1 and Atherosclerosis
 Nervous System Diseases Antibodies  >4 publications with INPPL1 and Nervous System Diseases
 Kidney Diseases Antibodies  >4 publications with INPPL1 and Kidney Diseases
 Liver Diseases Antibodies  >4 publications with INPPL1 and Liver Diseases
 Carcinoma Antibodies  >4 publications with INPPL1 and Carcinoma

Organs/Tissues associated with anti-INPPL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >133 publications with INPPL1 and Blood
 Bone Antibodies  >51 publications with INPPL1 and Bone
 Bone Marrow Antibodies  >47 publications with INPPL1 and Bone Marrow
 Muscle Antibodies  >25 publications with INPPL1 and Muscle
 Spleen Antibodies  >14 publications with INPPL1 and Spleen
 Liver Antibodies  >13 publications with INPPL1 and Liver
 Kidney Antibodies  >12 publications with INPPL1 and Kidney
 Heart Antibodies  >9 publications with INPPL1 and Heart
 Brain Antibodies  >9 publications with INPPL1 and Brain
 Lung Antibodies  >9 publications with INPPL1 and Lung
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