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BTC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751408

Description: Rabbit IgG polyclonal antibody for BTC detection. Tested with WB, IHC-P, ELISA (Cap) in Human.Background: Betacellulin is a protein that in humans is encoded by the BTC gene located on chromosome 4 at locus 4q13-q21. This gene encodes a member of… More details

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Product Overview

Online Datasheet in PDF
Product Name
BTC, Polyclonal Antibody
Full Product Name
Anti-BTC Antibody
Product Gene Name
anti-BTC antibody
Product Synonym Names
Probetacellulin; Betacellulin; BTC; BTC
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
19,746 Da
3D Structure
ModBase 3D Structure for P35070
Immunogen
E Coli-derived human BTC recombinant protein (Position: D32-Y111).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BTC antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA Capture (EIA)
Application Notes for anti-BTC antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
ELISA (Cap): 1-5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-BTC antibody

Figure 1. Western blot analysis of BTC using anti-BTC antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human BTC protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTC antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BTC at approximately 15KD. The expected band size for BTC is at 9KD.
Rabbit Anti-BTC Polyclonal Antibody – Cat# MBS1751408

Immunohistochemistry (IHC) of anti-BTC antibody

Figure 3. IHC analysis of BTC using anti-BTC antibody.
BTC was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BTC antibody.
BTC was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BTC Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-BTC Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751408

Product References and Citations for anti-BTC antibody

1. Elbein SC, Wang X, Karim MA, et al. (2006). "Analysis of coding variants in the betacellulin gene in type 2 diabetes and insulin secretion in African American subjects.". BMC Med. Genet. 7: 62. 2. Kim HS, Shin HS, Kwak HJ, Cho CH, Lee CO, Koh GY (February 2003). "Betacellulin induces angiogenesis through activation of mitogen-activated protein kinase and phosphatidylinositol 3'-kinase in endothelial cell". FASEB J. 17 (2): 318-20.

Other Notes

Small volumes of anti-BTC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTC purchase

MBS1751408 is an antibody targeting BTC. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BTC. It may not necessarily be applicable to this product.

NCBI GI #
948284407
NCBI GeneID
685
NCBI Accession #
NP_001303892.1
UniProt Primary Accession #
P35070
UniProt Secondary Accession #
Q96F48
UniProt Related Accession #
P35070
GenBank Nucleotide #
NM_001316963.1
NCBI Official Full Name
probetacellulin isoform 2
NCBI Official Synonym Full Names
betacellulin
NCBI Official Symbol
BTC
UniProt Protein Name
Probetacellulin
Protein Family
Probetacellulin
UniProt Gene Name
BTC
UniProt Synonym Gene Names
BTC
NCBI Protein Information
probetacellulin

NCBI Summary for BTC

This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients. [provided by RefSeq, Nov 2015]
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UniProt Comments for BTC

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Research Articles on BTC

1. Our results suggest that betacellulin induces ovarian cancer migration and Slug-dependent E-cadherin down-regulation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BTC antibody

 Reference Product  PubMed Publications
 EGFR antibody  >102 publications with BTC and EGFR
 AREG antibody  >67 publications with BTC and AREG
 ERBB2 antibody  >63 publications with BTC and ERBB2
 ERBB4 antibody  >46 publications with BTC and ERBB4
 HBEGF antibody  >44 publications with BTC and HBEGF
 ERBB3 antibody  >24 publications with BTC and ERBB3
 INS antibody  >9 publications with BTC and INS
 PAX4 antibody  >5 publications with BTC and PAX4
 IRS2 antibody  >1 publications with BTC and IRS2

Pathways associated with anti-BTC antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Signal Transduction Pathway antibodies  Diseases Of Signal Transduction Pathway Diagram

Diseases associated with anti-BTC antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >41 publications with BTC and Disease Models, Animal
 Liver Neoplasms Antibodies  >39 publications with BTC and Liver Neoplasms
 Liver Diseases Antibodies  >28 publications with BTC and Liver Diseases
 Inflammation Antibodies  >26 publications with BTC and Inflammation
 Anemia Antibodies  >20 publications with BTC and Anemia
 Breast Neoplasms Antibodies  >19 publications with BTC and Breast Neoplasms
 Immune System Diseases Antibodies  >19 publications with BTC and Immune System Diseases
 Neoplasm Metastasis Antibodies  >19 publications with BTC and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >19 publications with BTC and Neoplasms, Experimental
 Necrosis Antibodies  >16 publications with BTC and Necrosis

Organs/Tissues associated with anti-BTC antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >80 publications with BTC and Pancreas
 Lung Antibodies  >28 publications with BTC and Lung
 Kidney Antibodies  >19 publications with BTC and Kidney
 Stomach Antibodies  >9 publications with BTC and Stomach
 Uterus Antibodies  >6 publications with BTC and Uterus
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