Rabbit Anti-SOX3 Polyclonal Antibody – Cat# MBS1751413

Figure 1. Western blot analysis of SOX3 using anti-SOX3 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX3 at approximately 45KD. The expected band size for SOX3 is at 45KD.

SOX3 Antibody – Rabbit Polyclonal

Name: SOX3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1751413
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751413

Description: Rabbit IgG polyclonal antibody for SOX3 detection. Tested with WB in Human; Mouse; Rat.Background: Transcription factor SOX-3 is a protein that in humans is encoded by the SOX3 gene. This gene encodes a member of the SOX (SRY-related HMG-box)… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SOX3, Polyclonal Antibody

Full Product Name

Anti-SOX3 Antibody

Product Gene Name

anti-SOX3 antibody

Product Synonym Names

Transcription factor SOX-3; SOX3; SRY-box 3

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.

Molecular Weight

45,210 Da

3D Structure

ModBase 3D Structure for P41225

Immunogen

A synthetic peptide corresponding to a sequence of human SOX3 (DAADAASPLPGGRLHGVHQHYQGAGTAVNGTVPLTHI).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Relevant Detection Systems

It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SOX3 antibody

Western Blot (WB)

Application Notes for anti-SOX3 antibody

WB: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-SOX3 antibody

1. "Entrez Gene: SOX3 SRY (sex determining region Y)-box 3". 2. Woods KS, Cundall M, Turton J, Rizotti K, Mehta A, Palmer R, Wong J, Chong WK, Al-Zyoud M, El-Ali M, Otonkoski T, Martinez-Barbera JP, Thomas PQ, Robinson IC, Lovell-Badge R, Woodward KJ, Dattani MT (May 2005). "Over- and underdosage of SOX3 is associated with infundibular hypoplasia and hypopituitarism". American Journal of Human Genetics. 76 (5): 833-49.

Other Notes

Small volumes of anti-SOX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOX3 purchase

MBS1751413 is an antibody targeting SOX3. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX3. It may not necessarily be applicable to this product.

NCBI GI #

30061556

NCBI GeneID

6658

NCBI Accession #

NP_005625.2

UniProt Primary Accession #

P41225

UniProt Secondary Accession #

P35714; Q5JWI3; Q9NP49

UniProt Related Accession #

P41225

GenBank Nucleotide #

NM_005634.2

NCBI Official Full Name

transcription factor SOX-3

NCBI Official Synonym Full Names

SRY-box 3

NCBI Official Symbol

SOX3

NCBI Official Synonym Symbols

PHP; GHDX; MRGH; PHPX; SOXB

UniProt Protein Name

Transcription factor SOX-3

Protein Family

Transcription factor

UniProt Gene Name

SOX3

NCBI Protein Information

transcription factor SOX-3

NCBI Summary for SOX3

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked cognitive disability with growth hormone deficiency. [provided by RefSeq, Jul 2008]

UniProt Comments for SOX3

Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells ().

Research Articles on SOX3

1. Pathogenic missense mutation in SOX3 gene is associated with intellectual disability, microphthalmia, coloboma, hypopituitarism, facial dysmorphology and dental anomalies, including microcephaly, retrognathia and a solitary median maxillary central incisor amongst other features.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SOX3 antibody

Reference Product	PubMed Publications
PAX6 antibody	>20 publications with SOX3 and PAX6
DMRT1 antibody	>10 publications with SOX3 and DMRT1
NANOG antibody	>6 publications with SOX3 and NANOG
ATP11C antibody	>3 publications with SOX3 and ATP11C
NEUROG2 antibody	>2 publications with SOX3 and NEUROG2
MEIS1 antibody	>2 publications with SOX3 and MEIS1
ASB11 antibody	>1 publications with SOX3 and ASB11
NEUROG3 antibody	>1 publications with SOX3 and NEUROG3

Diseases associated with anti-SOX3 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>42 publications with SOX3 and Nervous System Diseases
Congenital Abnormalities Antibodies	>30 publications with SOX3 and Congenital Abnormalities
Urogenital Abnormalities Antibodies	>8 publications with SOX3 and Urogenital Abnormalities
Abnormalities, Multiple Antibodies	>8 publications with SOX3 and Abnormalities, Multiple
Disease Models, Animal Antibodies	>7 publications with SOX3 and Disease Models, Animal
Nervous System Malformations Antibodies	>5 publications with SOX3 and Nervous System Malformations
Panhypopituitarism X-linked Antibodies	>4 publications with SOX3 and Panhypopituitarism X-linked
Lung Diseases Antibodies	>3 publications with SOX3 and Lung Diseases
Mental Retardation, X-Linked, With Panhypopituitarism Antibodies	>3 publications with SOX3 and Mental Retardation, X-Linked, With Panhypopituitarism
Breast Neoplasms Antibodies	>2 publications with SOX3 and Breast Neoplasms

Organs/Tissues associated with anti-SOX3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>84 publications with SOX3 and Brain
Embryonic Tissue Antibodies	>53 publications with SOX3 and Embryonic Tissue
Testis Antibodies	>39 publications with SOX3 and Testis