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Caspase-2/CASP2 (CASP2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751419

Description: Rabbit IgG polyclonal antibody for Caspase-2 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Caspase-2/CASP2, Polyclonal Antibody
Full Product Name
Anti-Caspase-2/CASP2 Antibody
Product Gene Name
anti-CASP2 antibody
Product Synonym Names
Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12; CASP2; ICH1; NEDD2; Caspase 2, apoptosis-related cysteine peptidase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
34,923 Da
3D Structure
ModBase 3D Structure for P42575
Immunogen
E Coli-derived human Caspase-2 recombinant protein (Position: G170-T325).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CASP2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-CASP2 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-CASP2 antibody

Figure 1. Western blot analysis of Caspase-2 using anti-Caspase-2 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysate,
Lane 2: human 22RV1 whole cell lysate,
Lane 3: human SGC-7901 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-2 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase-2 at approximately 51KD. The expected band size for Caspase-2 is at 51KD.
Rabbit Anti-CASP2 Polyclonal Antibody – Cat# MBS1751419

Immunohistochemistry (IHC) of anti-CASP2 antibody

Figure 3. IHC analysis of Caspase-2 using anti-Caspase-2 antibody.
Caspase-2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.
Caspase-2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.
Caspase-2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.
Caspase-2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CASP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751419

Product References and Citations for anti-CASP2 antibody

1. Bergeron, L.; Perez, G. I.; Macdonald, G.; Shi, L.; Sun, Y.; Jurisicova, A.; Varmuza, S.; Latham, K. E.; Flaws, J. A.; Salter, J. C. M.; Hara, H.; Moskowitz, M. A.; Li, E.; Greenberg, A.; Tilly, J. L.; Yuan, J.: Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 12: 1304-1314, 1998. 2. Tinel, A.; Tschopp, J.: The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. Science 304: 843-846, 2004.

Other Notes

Small volumes of anti-CASP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASP2 purchase

MBS1751419 is an antibody targeting CASP2. Also known as Anti-Caspase-2/CASP2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CASP2. It may not necessarily be applicable to this product.

NCBI GI #
4502575
NCBI GeneID
835
NCBI Accession #
NP_001215.1
UniProt Primary Accession #
P42575
UniProt Secondary Accession #
P42576; Q59F21; Q7KZL6; Q86UJ3; Q9BUP7; Q9BZK9; Q9BZL0; A8K5F9; D3DXD6; E9PDN0
UniProt Related Accession #
P42575
GenBank Nucleotide #
NM_001224.4
NCBI Official Full Name
caspase-2 isoform 2
NCBI Official Synonym Full Names
caspase 2
NCBI Official Symbol
CASP2
NCBI Official Synonym Symbols
ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
UniProt Protein Name
Caspase-2
UniProt Synonym Protein Names
Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2
Protein Family
Caspase
UniProt Gene Name
CASP2
UniProt Synonym Gene Names
ICH1; NEDD2; CASP-2; NEDD-2
NCBI Protein Information
caspase-2

NCBI Summary for CASP2

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
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UniProt Comments for CASP2

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).
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Research Articles on CASP2

1. these data identify a novel caspase-2-interacting factor, FAN, and expand the role for the enzyme in seemingly non-apoptotic cellular mechanisms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CASP2 antibody

 Reference Product  PubMed Publications
 BID antibody  >11 publications with CASP2 and BID
 CRADD antibody  >9 publications with CASP2 and CRADD
 PIDD antibody  >7 publications with CASP2 and PIDD
 CASP7 antibody  >7 publications with CASP2 and CASP7
 CFLAR antibody  >2 publications with CASP2 and CFLAR
 CASP10 antibody  >2 publications with CASP2 and CASP10
 NOL3 antibody  >1 publications with CASP2 and NOL3

Pathways associated with anti-CASP2 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Apoptosis Modulation By HSP70 Pathway antibodies  Apoptosis Modulation By HSP70 Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway antibodies  HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 NADE Modulates Death Signalling Pathway antibodies  NADE Modulates Death Signalling Pathway Diagram

Diseases associated with anti-CASP2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >40 publications with CASP2 and Neoplasms
 Necrosis Antibodies  >8 publications with CASP2 and Necrosis
 Disease Models, Animal Antibodies  >7 publications with CASP2 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >7 publications with CASP2 and Cardiovascular Diseases
 Drug Toxicity Antibodies  >6 publications with CASP2 and Drug Toxicity
 Brain Diseases Antibodies  >6 publications with CASP2 and Brain Diseases
 Nervous System Diseases Antibodies  >6 publications with CASP2 and Nervous System Diseases
 Breast Neoplasms Antibodies  >4 publications with CASP2 and Breast Neoplasms
 Lung Diseases Antibodies  >3 publications with CASP2 and Lung Diseases
 Lung Neoplasms Antibodies  >3 publications with CASP2 and Lung Neoplasms

Organs/Tissues associated with anti-CASP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with CASP2 and Blood
 Brain Antibodies  >10 publications with CASP2 and Brain
 Nerve Antibodies  >6 publications with CASP2 and Nerve
 Vascular Antibodies  >4 publications with CASP2 and Vascular
 Bone Antibodies  >4 publications with CASP2 and Bone
 Lung Antibodies  >4 publications with CASP2 and Lung
 Bone Marrow Antibodies  >3 publications with CASP2 and Bone Marrow
 Liver Antibodies  >3 publications with CASP2 and Liver
 Prostate Antibodies  >3 publications with CASP2 and Prostate
 Thymus Antibodies  >2 publications with CASP2 and Thymus
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