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CUL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751442

Description: Rabbit IgG polyclonal antibody for CUL2 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Cullin 2 (CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, it is showed that CUL2 is a… More details

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Product Overview

Online Datasheet in PDF
Product Name
CUL2, Polyclonal Antibody
Full Product Name
Anti-CUL2 Antibody
Product Gene Name
anti-CUL2 antibody
Product Synonym Names
Cullin-2; CUL-2; CUL2; Cullin 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
89,492 Da
3D Structure
ModBase 3D Structure for Q13617
Immunogen
E Coli-derived human CUL2 recombinant protein (Position: E389-A745).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CUL2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-CUL2 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Western Blot (WB) of anti-CUL2 antibody

Figure 1. Western blot analysis of CUL2 using anti-CUL2 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysate,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL2 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUL2 at approximately 87KD. The expected band size for CUL2 is at 87KD.
Rabbit Anti-CUL2 Polyclonal Antibody – Cat# MBS1751442

Product References and Citations for anti-CUL2 antibody

1. Clifford, S. C., Walsh, S., Hewson, K., Green, E. K., Brinke, A., Green, P. M., Gianelli, F., Eng, C., Maher, E. R. Genomic organization and chromosomal localization of the human CUL2 gene and the role of von Hippel-Lindau tumor suppressor-binding protein (CUL2 and VBP1) mutation and loss in renal-cell carcinoma development. Genes Chromosomes Cancer 26: 20-28, 1999. 2. Lonergan, K. M., Iliopoulos, O., Ohh, M., Kamura, T., Conaway, R. C., Conaway, J. W., Kaelin, W. G., Jr.Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2. Molec. Cell. Biol. 18: 732-741, 1998. 3. Pause, A., Lee, S., Worrell, R. A., Chen, D. Y. T., Burgess, W. H., Linehan, W. M., Klausner, R. D. The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Nat. Acad. Sci. 94: 2156-2161, 1997.

Other Notes

Small volumes of anti-CUL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CUL2 purchase

MBS1751442 is an antibody targeting CUL2. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CUL2. It may not necessarily be applicable to this product.

NCBI GI #
311771637
NCBI GeneID
8453
NCBI Accession #
NP_001185706.1
UniProt Primary Accession #
Q13617
UniProt Secondary Accession #
O00200; Q5T2B6; Q9UNF9; B3KT95; B7Z6K8; D3DRY6; G3V1S2
UniProt Related Accession #
Q13617
GenBank Nucleotide #
NM_001198777.1
NCBI Official Full Name
cullin-2 isoform c
NCBI Official Synonym Full Names
cullin 2
NCBI Official Symbol
CUL2
UniProt Protein Name
Cullin-2
Protein Family
Cullin
UniProt Gene Name
CUL2
UniProt Synonym Gene Names
CUL-2
NCBI Protein Information
cullin-2

UniProt Comments for CUL2

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
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Research Articles on CUL2

1. direct evidence that both A3A and HPV16 E7 interact with CUL2, suggesting that the E7-CUL2 complex formed during HPV infection may regulate A3A protein levels in the cell.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CUL2 antibody

 Reference Product  PubMed Publications
 VHL antibody  >53 publications with CUL2 and VHL
 NEDD8 antibody  >21 publications with CUL2 and NEDD8
 CAND1 antibody  >6 publications with CUL2 and CAND1
 COMMD1 antibody  >4 publications with CUL2 and COMMD1
 RNF7 antibody  >3 publications with CUL2 and RNF7
 ZER1 antibody  >1 publications with CUL2 and ZER1
 TCEB1 antibody  >1 publications with CUL2 and TCEB1

Pathways associated with anti-CUL2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ECV Complex Pathway antibodies  ECV Complex Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway antibodies  Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram

Diseases associated with anti-CUL2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >24 publications with CUL2 and Neoplasms
 Carcinoma Antibodies  >17 publications with CUL2 and Carcinoma
 Kidney Diseases Antibodies  >14 publications with CUL2 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >13 publications with CUL2 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >12 publications with CUL2 and Nervous System Diseases
 Adenocarcinoma Antibodies  >12 publications with CUL2 and Adenocarcinoma
 Metaplasia Antibodies  >2 publications with CUL2 and Metaplasia
 Drug Toxicity Antibodies  >2 publications with CUL2 and Drug Toxicity
 Inflammation Antibodies  >2 publications with CUL2 and Inflammation
 Congenital Abnormalities Antibodies  >2 publications with CUL2 and Congenital Abnormalities

Organs/Tissues associated with anti-CUL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >18 publications with CUL2 and Kidney
 Vascular Antibodies  >12 publications with CUL2 and Vascular
 Liver Antibodies  >6 publications with CUL2 and Liver
 Blood Antibodies  >5 publications with CUL2 and Blood
 Intestine Antibodies  >2 publications with CUL2 and Intestine
 Adrenal Gland Antibodies  >2 publications with CUL2 and Adrenal Gland
 Eye Antibodies  >1 publications with CUL2 and Eye
 Muscle Antibodies  >1 publications with CUL2 and Muscle
 Placenta Antibodies  >1 publications with CUL2 and Placenta
 Embryonic Tissue Antibodies  >1 publications with CUL2 and Embryonic Tissue
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