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P Cadherin/CDH3 (CDH3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751454

Description: Rabbit IgG polyclonal antibody for P cadherin detection. Tested with WB, IHC-P, IHC-F, Direct ELISA in Human; Mouse; Rat.Background: Cadherins, such as CDH3, are integral membrane glycoproteins responsible for calcium-dependent cell-cell… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
P cadherin/CDH3, Polyclonal Antibody
Full Product Name
Anti-P cadherin/CDH3 Antibody
Product Gene Name
anti-CDH3 antibody
Product Synonym Names
Cadherin-3; Placental cadherin; P-cadherin; CDH3; CDHP; Cadherin 3, type 1, P-cadherin (placental)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
86,552 Da
3D Structure
ModBase 3D Structure for P22223
Immunogen
E Coli-derived human P cadherin recombinant protein (Position: Q126-H336).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P and IHC (F).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CDH3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Direct ELISA (EIA)
Application Notes for anti-CDH3 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
IHC-F: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-CDH3 antibody

Figure 1. Western blot analysis of P cadherin using anti-P cadherin antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human K562 whole cell lysate,
Lane 3: human A431 whole cell lysate,
Lane 4: human Caco-2 whole cell lysate,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P cadherin antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P cadherin at approximately 120KD. The expected band size for P cadherin is at 91KD.
Rabbit Anti-CDH3 Polyclonal Antibody – Cat# MBS1751454

Immunohistochemistry (IHC) of anti-CDH3 antibody

Figure 3. IHC analysis of P cadherin using anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin antibody.
P cadherin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-P cadherin Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CDH3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751454

Product References and Citations for anti-CDH3 antibody

1. Kaupmann K, Becker-Follmann J, Scherer G, Jockusch H, Starzinski-Powitz A (Dec 1992). "The gene for the cell adhesion molecule M-cadherin maps to mouse chromosome 8 and human chromosome 16q24.1-qter and is near the E-cadherin (uvomorulin) locus in both species". Genomics 14 (2): 488-90.

Other Notes

Small volumes of anti-CDH3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDH3 purchase

MBS1751454 is an antibody targeting CDH3. Also known as Anti-P cadherin/CDH3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH3. It may not necessarily be applicable to this product.

NCBI GI #
953953841
NCBI GeneID
1001
NCBI Accession #
NP_001304124.1
UniProt Primary Accession #
P22223
UniProt Secondary Accession #
Q05DI6; B2R6F4
UniProt Related Accession #
P22223
GenBank Nucleotide #
NM_001317195.1
NCBI Official Full Name
cadherin-3 isoform 2
NCBI Official Synonym Full Names
cadherin 3
NCBI Official Symbol
CDH3
NCBI Official Synonym Symbols
CDHP; HJMD; PCAD
UniProt Protein Name
Cadherin-3
UniProt Synonym Protein Names
Placental cadherin; P-cadherin
UniProt Gene Name
CDH3
UniProt Synonym Gene Names
CDHP; P-cadherin
NCBI Protein Information
cadherin-3

NCBI Summary for CDH3

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]
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UniProt Comments for CDH3

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Research Articles on CDH3

1. the evaluation of P-cadherin expression in lymph node metastases is an important predictor of disease outcome in breast cancer axillary lymph node metastases

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDH3 antibody

 Reference Product  PubMed Publications
 CDH1 antibody  >23 publications with CDH3 and CDH1
 CTNNB1 antibody  >4 publications with CDH3 and CTNNB1
 CDH11 antibody  >3 publications with CDH3 and CDH11
 CDH5 antibody  >2 publications with CDH3 and CDH5
 CDH13 antibody  >2 publications with CDH3 and CDH13
 CDH8 antibody  >1 publications with CDH3 and CDH8

Pathways associated with anti-CDH3 antibody

 Products by Pathway  Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram

Diseases associated with anti-CDH3 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >15 publications with CDH3 and Breast Neoplasms
 Carcinoma, Squamous Cell Antibodies  >6 publications with CDH3 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >5 publications with CDH3 and Lung Neoplasms
 Lung Diseases Antibodies  >5 publications with CDH3 and Lung Diseases
 Neoplasm Metastasis Antibodies  >5 publications with CDH3 and Neoplasm Metastasis
 Ectodermal dysplasia, ectrodactyly, and macular dystrophy Antibodies  >5 publications with CDH3 and Ectodermal dysplasia, ectrodactyly, and macular dystrophy
 Neoplasms, Experimental Antibodies  >4 publications with CDH3 and Neoplasms, Experimental
 Inflammation Antibodies  >4 publications with CDH3 and Inflammation
 Chromosome Aberrations Antibodies  >4 publications with CDH3 and Chromosome Aberrations
 Precancerous Conditions Antibodies  >3 publications with CDH3 and Precancerous Conditions

Organs/Tissues associated with anti-CDH3 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >9 publications with CDH3 and Eye
 Skin Antibodies  >8 publications with CDH3 and Skin
 Lung Antibodies  >6 publications with CDH3 and Lung
 Liver Antibodies  >6 publications with CDH3 and Liver
 Intestine Antibodies  >6 publications with CDH3 and Intestine
 Blood Antibodies  >5 publications with CDH3 and Blood
 Stomach Antibodies  >4 publications with CDH3 and Stomach
 Embryonic Tissue Antibodies  >4 publications with CDH3 and Embryonic Tissue
 Prostate Antibodies  >3 publications with CDH3 and Prostate
 Thyroid Antibodies  >3 publications with CDH3 and Thyroid
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