• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LMAN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751459

Description: Rabbit IgG polyclonal antibody for LMAN1 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Protein ERGIC-53 also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1 is a protein that… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LMAN1, Polyclonal Antibody
Full Product Name
Anti-LMAN1 Antibody
Product Gene Name
anti-LMAN1 antibody
Product Synonym Names
Protein ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; LMAN1; ERGIC53; F5F8D; Lectin, mannose binding 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
57,549 Da
3D Structure
ModBase 3D Structure for P49257
Immunogen
E Coli-derived human LMAN1 recombinant protein (Position: K96-M458).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-LMAN1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-LMAN1 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-LMAN1 antibody

Figure 1. Western blot analysis of LMAN1 using anti-LMAN1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysate,
Lane 2: human A431 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58KD. The expected band size for LMAN1 is at 58KD.
Rabbit Anti-LMAN1 Polyclonal Antibody – Cat# MBS1751459

Immunohistochemistry (IHC) of anti-LMAN1 antibody

Figure 3. IHC analysis of LMAN1 using anti-LMAN1 antibody.
LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.
LMAN1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.
LMAN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.
LMAN1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 antibody.
LMAN1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LMAN1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-LMAN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751459

Product References and Citations for anti-LMAN1 antibody

1. Neve, E. P. A., Svensson, K., Fuxe, J., Petterson, R. F. VIPL, a VIP36-like membrane protein with a putative function in the export of glycoproteins from the endoplasmic reticulum. Exp. Cell Res. 288: 70-83, 2003. 2. Zhang, B., Spreafico, M., Zheng, C., Yang, A., Platzer, P., Callaghan, M. U., Avci, Z., Ozbek, N., Mahlangu, J., Haw, T., Kaufman, R. J., Marchant, K., Tuddenham, E. G. D., Seligsohn, U., Peyvandi, F., Ginsburg, D. Genotype-phenotype correlation in combined deficiency of factor V and factor VIII. Blood 111: 5592-5600, 2008.

Other Notes

Small volumes of anti-LMAN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LMAN1 purchase

MBS1751459 is an antibody targeting LMAN1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LMAN1. It may not necessarily be applicable to this product.

NCBI GI #
5031873
NCBI GeneID
3998
NCBI Accession #
NP_005561.1
UniProt Primary Accession #
P49257
UniProt Secondary Accession #
Q12895; Q8N5I7; Q9UQG1; Q9UQG2; Q9UQG3; Q9UQG4; Q9UQG5; Q9UQG6; Q9UQG7; Q9UQG8; Q9UQG9
UniProt Related Accession #
P49257
GenBank Nucleotide #
NM_005570.4
NCBI Official Full Name
protein ERGIC-53
NCBI Official Synonym Full Names
lectin, mannose binding 1
NCBI Official Symbol
LMAN1
NCBI Official Synonym Symbols
MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53
UniProt Protein Name
Protein ERGIC-53
UniProt Synonym Protein Names
ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1
Protein Family
Protein
UniProt Gene Name
LMAN1
UniProt Synonym Gene Names
ERGIC53; F5F8D
NCBI Protein Information
protein ERGIC-53

NCBI Summary for LMAN1

The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015]
Show more

UniProt Comments for LMAN1

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Show more

Research Articles on LMAN1

1. Combined deficiency of factors V and VIII by chance coinheritance of parahaemophilia and haemophilia A, but not by mutations of either LMAN1 or MCFD2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LMAN1 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway antibodies  Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway Diagram
 Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway antibodies  Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway Diagram
 Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway antibodies  Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway Diagram
 Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway antibodies  Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway Diagram
 Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway antibodies  Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway Diagram
 Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway antibodies  Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway Diagram
 Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway antibodies  Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway Diagram
 Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway antibodies  Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway Diagram
 Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway antibodies  Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway Diagram

Diseases associated with anti-LMAN1 antibody

 Disease Name  Pubmed Publications
 FACTOR V AND FACTOR VIII, COMBINED DEFICIENCY OF, 1 Antibodies  >19 publications with LMAN1 and FACTOR V AND FACTOR VIII, COMBINED DEFICIENCY OF, 1
 Hemorrhage Antibodies  >8 publications with LMAN1 and Hemorrhage
 Inflammation Antibodies  >2 publications with LMAN1 and Inflammation
 Poisoning Antibodies  >1 publications with LMAN1 and Poisoning
 Fibrosis Antibodies  >1 publications with LMAN1 and Fibrosis
 Kidney Diseases Antibodies  >1 publications with LMAN1 and Kidney Diseases
 Liver Diseases Antibodies  >1 publications with LMAN1 and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with LMAN1 and Liver Neoplasms
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with LMAN1 and Prenatal Exposure Delayed Effects
 Carcinoma, Hepatocellular Antibodies  >1 publications with LMAN1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-LMAN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with LMAN1 and Blood
 Kidney Antibodies  >6 publications with LMAN1 and Kidney
 Liver Antibodies  >5 publications with LMAN1 and Liver
 Brain Antibodies  >4 publications with LMAN1 and Brain
 Skin Antibodies  >3 publications with LMAN1 and Skin
 Bone Antibodies  >3 publications with LMAN1 and Bone
 Eye Antibodies  >2 publications with LMAN1 and Eye
 Muscle Antibodies  >2 publications with LMAN1 and Muscle
 Bone Marrow Antibodies  >1 publications with LMAN1 and Bone Marrow
 Testis Antibodies  >1 publications with LMAN1 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000