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GM130 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751495

Description: Rabbit IgG polyclonal antibody for GM130 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, Direct ELISA in Human; Mouse; Rat.Background: Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus,… More details

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Product Overview

Online Datasheet in PDF
Product Name
GM130/GOLGA2, Polyclonal Antibody
Full Product Name
Anti-GM130/GOLGA2 Antibody
Product Gene Name
anti-GM130 antibody
Product Synonym Gene Name
GOLGA2
Product Synonym Names
Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; Golgin A2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
70,473 Da
3D Structure
ModBase 3D Structure for Q08379
Immunogen
E Coli-derived human GM130 recombinant protein (Position: E796-E913).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P, IHC-F and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-GM130 antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA)
Application Notes for anti-GM130 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
IHC-F: 0.5-1 mug/ml
ICC/IF: 2 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-GM130 antibody

Figure 1. Western blot analysis of GM130 using anti-GM130 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysate,
Lane 3: human K562 whole cell lysate,
Lane 4: human HL-60 whole cell lysate,
Lane 5: human MCF-7 whole cell lysate,
Lane 6: human Caco-2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GM130 at approximately 150KD. The expected band size for GM130 is at 113KD.
Rabbit Anti-GM130 Polyclonal Antibody – Cat# MBS1751495

Immunohistochemistry (IHC) of anti-GM130 antibody

Figure 3. IHC analysis of GM130 using anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 antibody.
GM130 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GM130 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-GM130 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751495

Product References and Citations for anti-GM130 antibody

1. Matsuki, T., Matthews, R. T., Cooper, J. A., van der Brug, M. P., Cookson, M. R., Hardy, J. A., Olson, E. C., Howell, B. W. Reelin and Stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 143: 826-836, 2010. 2. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 601 only, 2014.

Other Notes

Small volumes of anti-GM130 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM130 purchase

MBS1751495 is an antibody targeting GM130. Also known as Anti-GM130/GOLGA2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GM130. It may not necessarily be applicable to this product.

NCBI GI #
194097392
NCBI GeneID
2801
NCBI Accession #
NP_004477.3
UniProt Primary Accession #
Q08379
UniProt Secondary Accession #
Q6GRM9; Q9BRB0; Q9NYF9; A0A0C4DGS5
UniProt Related Accession #
Q08379
GenBank Nucleotide #
NM_004486.4
NCBI Official Full Name
golgin subfamily A member 2
NCBI Official Synonym Full Names
golgin A2
NCBI Official Symbol
GOLGA2
NCBI Official Synonym Symbols
GM130
UniProt Protein Name
Golgin subfamily A member 2
UniProt Synonym Protein Names
130 kDa cis-Golgi matrix protein
UniProt Gene Name
GOLGA2
UniProt Synonym Gene Names
GM1302 PublicationsManual assertion based on opinion iniRef.1
NCBI Protein Information
golgin subfamily A member 2

NCBI Summary for GM130

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]
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UniProt Comments for GM130

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
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Research Articles on GM130

1. In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GM130 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Golgi Cisternae Pericentriolar Stack Reorganization Pathway antibodies  Golgi Cisternae Pericentriolar Stack Reorganization Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Prophase Pathway antibodies  Mitotic Prophase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram

Diseases associated with anti-GM130 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1 publications with GM130 and Immune System Diseases
 Carcinoma Antibodies  >1 publications with GM130 and Carcinoma
 Lung Neoplasms Antibodies  >1 publications with GM130 and Lung Neoplasms
 Neoplasms Antibodies  >1 publications with GM130 and Neoplasms
 Adenocarcinoma Antibodies  >1 publications with GM130 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >1 publications with GM130 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >1 publications with GM130 and Necrosis

Organs/Tissues associated with anti-GM130 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >3 publications with GM130 and Nerve
 Eye Antibodies  >2 publications with GM130 and Eye
 Blood Antibodies  >1 publications with GM130 and Blood
 Heart Antibodies  >1 publications with GM130 and Heart
 Muscle Antibodies  >1 publications with GM130 and Muscle
 Lung Antibodies  >1 publications with GM130 and Lung
 Liver Antibodies  >1 publications with GM130 and Liver
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