Rabbit Anti-HOOK3 Polyclonal Antibody – Cat# MBS1751510

HOOK3 Antibody – Rabbit Polyclonal

Name: HOOK3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1751510
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751510

Description: Rabbit IgG polyclonal antibody for HOOK3 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Protein Hook homolog 3 is a protein that in humans is encoded by the HOOK3 gene. Hook proteins are cytosolic coiled-coil… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HOOK3, Polyclonal Antibody

Full Product Name

Anti-HOOK3 Antibody

Product Gene Name

anti-HOOK3 antibody

Product Synonym Names

Protein Hook homolog 3; h-hook3; Hhk3; HOOK3; Hook microtubule tethering protein 3

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.

Molecular Weight

83,126 Da

3D Structure

ModBase 3D Structure for Q86VS8

Immunogen

E Coli-derived human HOOK3 recombinant protein (Position: E14-R198).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500mug/ml.

Relevant Detection Systems

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-HOOK3 antibody

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)

Application Notes for anti-HOOK3 antibody

WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.

Product References and Citations for anti-HOOK3 antibody

1. Urnavicius, L., Lau, C. K., Elshenawy, M. M., Morales-Rios, E., Motz, C., Yildiz, A., Carter, A. P. Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature 554: 202-206, 2018. 2. Walenta, J. H., Didier, A. J., Liu, X., Kramer, H. The Golgi-associated Hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 152: 923-934, 2001.

Other Notes

Small volumes of anti-HOOK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HOOK3 purchase

MBS1751510 is an antibody targeting HOOK3. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HOOK3. It may not necessarily be applicable to this product.

NCBI GI #

14165274

NCBI GeneID

84376

NCBI Accession #

NP_115786.1

UniProt Primary Accession #

Q86VS8

UniProt Secondary Accession #

Q8NBH0; Q9BY13; D3DSY8

UniProt Related Accession #

Q86VS8

GenBank Nucleotide #

NM_032410.4

NCBI Official Full Name

protein Hook homolog 3

NCBI Official Synonym Full Names

hook microtubule tethering protein 3

NCBI Official Symbol

HOOK3

NCBI Official Synonym Symbols

HK3

UniProt Protein Name

Protein Hook homolog 3

Protein Family

Protein Hook

UniProt Gene Name

HOOK3

UniProt Synonym Gene Names

h-hook3; hHK3

NCBI Protein Information

protein Hook homolog 3

NCBI Summary for HOOK3

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM, Apr 2004]

UniProt Comments for HOOK3

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).

Research Articles on HOOK3

1. This work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-HOOK3 antibody

Disease Name	Pubmed Publications
Prostatic Diseases Antibodies	>1 publications with HOOK3 and Prostatic Diseases

Organs/Tissues associated with anti-HOOK3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with HOOK3 and Brain
Thyroid Antibodies	>2 publications with HOOK3 and Thyroid
Blood Antibodies	>2 publications with HOOK3 and Blood
Prostate Antibodies	>2 publications with HOOK3 and Prostate
Intestine Antibodies	>1 publications with HOOK3 and Intestine
Lymph Node Antibodies	>1 publications with HOOK3 and Lymph Node
Kidney Antibodies	>1 publications with HOOK3 and Kidney
Embryonic Tissue Antibodies	>1 publications with HOOK3 and Embryonic Tissue
Lung Antibodies	>1 publications with HOOK3 and Lung
Uncharacterized Tissue Antibodies	>1 publications with HOOK3 and Uncharacterized Tissue