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TMEM199 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751525

Description: Rabbit IgG polyclonal antibody for TMEM199 detection. Tested with WB, Direct ELISA in Human; Mouse; Rat.Background: TMEM199 encodes a protein homologous to the yeast V-ATPase assembly factor Vma12 and appears to be involved in Golgi homeostasis.… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TMEM199, Polyclonal Antibody
Full Product Name
Anti-TMEM199 Antibody
Product Gene Name
anti-TMEM199 antibody
Product Synonym Names
Transmembrane protein 199; TMEM199; C17orf32
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
23,130 Da
3D Structure
ModBase 3D Structure for Q8N511
Immunogen
E Coli-derived human TMEM199 recombinant protein (Position: E20-H129).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TMEM199 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes for anti-TMEM199 antibody
WB: 0.1-0.5ug/ml
Direct ELISA: 0.1-0.5ug/ml
Tested Species: In-house tested species with positive results. Other applications have not been tested.

Product References and Citations for anti-TMEM199 antibody

1. Calvo, P. L., Pagliardini, S., Baldi, M., Pucci, A., Sturiale, L., Garozzo, D., Vinciguerra, T., Barbera, C., Jaeken, J. Long-standing mild hypertransaminasaemia caused by congenital disorder of glycosylation (CDG) type IIx. J. Inherit. Metab. Dis. 31: S437-S440, 2008. 2. Jansen, J. C., Timal, S., van Scherpenzeel, M., Michelakakis, H., Vicogne, D., Ashikov, A., Moraitou, M., Hoischen, A., Huijben, K., Steenbergen, G., van den Boogert, M. A. W., Porta, F., and 14 others. TMEM199 deficiency is a disorder of Golgi homeostasis characterized by elevated aminotransferases, alkaline phosphatase, and cholesterol and abnormal glycosylation. Am. J. Hum. Genet. 98: 322-330, 2016.

Other Notes

Small volumes of anti-TMEM199 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TMEM199 purchase

MBS1751525 is an antibody targeting TMEM199. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TMEM199. It may not necessarily be applicable to this product.

NCBI GI #
22748979
NCBI GeneID
147007
NCBI Accession #
NP_689677.1
UniProt Primary Accession #
Q8N511
UniProt Related Accession #
Q8N511
GenBank Nucleotide #
NM_152464.3
NCBI Official Full Name
transmembrane protein 199
NCBI Official Synonym Full Names
transmembrane protein 199
NCBI Official Symbol
TMEM199
NCBI Official Synonym Symbols
VPH2; CDG2P; VMA12; C17orf32
UniProt Protein Name
Transmembrane protein 199
Protein Family
Transmembrane protein
UniProt Gene Name
TMEM199
NCBI Protein Information
transmembrane protein 199

NCBI Summary for TMEM199

The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. The encoded protein shares some homology with the yeast protein Vma12. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. [provided by RefSeq, Mar 2016]
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UniProt Comments for TMEM199

Accessory component of the proton-transporting vacuolar (V)-ATPase protein pump involved in intracellular iron homeostasis. In aerobic conditions, required for intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. Necessary for endolysosomal acidification and lysosomal degradation (PubMed:28296633). May be involved in Golgi homeostasis (PubMed:26833330).
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Research Articles on TMEM199

1. Vacuolar H+ ATPase (V-ATPase), the key proton pump for endo-lysosomal acidification, and two previously uncharacterised V-ATPase assembly factors, TMEM199 and CCDC115, stabilise HIF1alpha in aerobic conditions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TMEM199 antibody

 Disease Name  Pubmed Publications
 Cognition Disorders Antibodies  >1 publications with TMEM199 and Cognition Disorders
 Liver Diseases Antibodies  >1 publications with TMEM199 and Liver Diseases

Organs/Tissues associated with anti-TMEM199 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with TMEM199 and Brain
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