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RFC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175156

Description: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.Background: RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct… More details

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Product Overview

Online Datasheet in PDF
Product Name
RFC1, Polyclonal Antibody
Full Product Name
Anti-RFC1 antibody
Product Gene Name
anti-RFC1 antibody
Product Synonym Names
Replication factor C subunit 1; replication factor C(activator 1) 1, 145kDa; A1 140 kDa subunit antibody; A1 antibody; A1 P145 Activator 1 large subunit antibody; Activator 1 140 kDa subunit antibody; Activator 1 large subunit antibody; Activator 1 subunit 1 antibody; DNA binding Protein PO GA antibody; DNA-binding protein PO-GA antibody; MHC binding factor beta antibody; MHCBFB antibody; PO GA antibody; RECC1 antibody; Replication factor C(activator 1) 1, 145kDa antibody; Replication factor C 140 kDa subunit antibody; Replication factor C antibody; Replication factor C large subunit antibody; Replication factor C subunit 1 antibody; Replication factor C1 antibody; RF-C 140 kDa subunit antibody; RFC antibody; RFC1 antibody; RFC1_HUMAN antibody; RFC140 antibody; RFC140 Replication Factor C 140 kDa subunit antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
128,183 Da
OMIM
102579
3D Structure
ModBase 3D Structure for P35251
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1 (46-64aa NSSRKEDDFKQKQPSKKKR).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RFC1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB) of anti-RFC1 antibody

Anti-RFC1 antibody, MBS175156, Western blotting
All lanes: Anti RFC1 (MBS175156) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: CEM Whole Cell Lysate at 40ug
Lane 5: 293T Whole Cell Lysate at 40ug
Predicted bind size: 128KD
Observed bind size: 150KD
Rabbit Anti-RFC1 Polyclonal Antibody – Cat# MBS175156

Immunohistochemistry (IHC) of anti-RFC1 antibody

Anti-RFC1 antibody, MBS175156, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-RFC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175156

Other Notes

Small volumes of anti-RFC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFC1 purchase

MBS175156 is an antibody targeting RFC1. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFC1. It may not necessarily be applicable to this product.

NCBI GI #
56757608
NCBI GeneID
5981
NCBI Accession #
P35251.4
UniProt Primary Accession #
P35251
UniProt Secondary Accession #
Q5XKF5; Q6PKU0; Q86V41; Q86V46; A8K6E7
UniProt Related Accession #
P35251
NCBI Official Full Name
Replication factor C subunit 1
NCBI Official Synonym Full Names
replication factor C (activator 1) 1, 145kDa
NCBI Official Symbol
RFC1
NCBI Official Synonym Symbols
A1; RFC; PO-GA; RECC1; MHCBFB; RFC140
UniProt Protein Name
Replication factor C subunit 1
UniProt Synonym Protein Names
Activator 1 140 kDa subunit; A1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; Replication factor C 140 kDa subunit; RF-C 140 kDa subunit; RFC140; Replication factor C large subunit
Protein Family
Probable replication factor
UniProt Gene Name
RFC1
UniProt Synonym Gene Names
RFC140; A1 140 kDa subunit; RF-C 140 kDa subunit; RFC140
UniProt Entry Name
RFC1_HUMAN
NCBI Protein Information
replication factor C subunit 1; A1 140 kDa subunit; RF-C 140 kDa subunit; activator 1 subunit 1; replication factor C1; MHC binding factor, beta; DNA-binding protein PO-GA; activator 1 large subunit; activator 1 140 kDa subunit; replication factor C large subunit; replication factor C 140 kDa subunit

NCBI Summary for RFC1

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
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UniProt Comments for RFC1

RFC1: a DNA binding protein that may play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and RFC1. The RFC1 subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Two alternatively spliced isoforms have been described.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 4p14-p13

Cellular Component: nucleoplasm; Golgi apparatus; DNA replication factor C complex; nucleolus; nucleus; cell junction

Molecular Function: protein domain specific binding; protein binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; enzyme activator activity; ATP binding; DNA clamp loader activity

Biological Process: positive regulation of catalytic activity; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; telomere maintenance via telomerase; negative regulation of transcription from RNA polymerase II promoter; DNA strand elongation during DNA replication; DNA repair; telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; telomere maintenance
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Research Articles on RFC1

1. RFC depletion had a negative impact on LANA's ability to replicate and maintain viral DNA in cells containing artificial KSHV episomes or in infected cells, leading to loss of virus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RFC1 antibody

 Reference Product  PubMed Publications
 PCNA antibody  >46 publications with RFC1 and PCNA
 RFC2 antibody  >18 publications with RFC1 and RFC2
 RFC5 antibody  >12 publications with RFC1 and RFC5
 RFC3 antibody  >11 publications with RFC1 and RFC3
 RFC4 antibody  >9 publications with RFC1 and RFC4
 POLE antibody  >1 publications with RFC1 and POLE
 POLD1 antibody  >1 publications with RFC1 and POLD1

Pathways associated with anti-RFC1 antibody

 Products by Pathway  Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-RFC1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >29 publications with RFC1 and Nervous System Diseases
 Heart Diseases Antibodies  >7 publications with RFC1 and Heart Diseases
 Vascular Diseases Antibodies  >6 publications with RFC1 and Vascular Diseases
 Lung Neoplasms Antibodies  >5 publications with RFC1 and Lung Neoplasms
 Liver Diseases Antibodies  >4 publications with RFC1 and Liver Diseases
 Death Antibodies  >4 publications with RFC1 and Death
 Kidney Diseases Antibodies  >3 publications with RFC1 and Kidney Diseases
 Colonic Neoplasms Antibodies  >3 publications with RFC1 and Colonic Neoplasms
 Poisoning Antibodies  >2 publications with RFC1 and Poisoning
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with RFC1 and Drug-Related Side Effects and Adverse Reactions

Organs/Tissues associated with anti-RFC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >62 publications with RFC1 and Blood
 Heart Antibodies  >11 publications with RFC1 and Heart
 Liver Antibodies  >10 publications with RFC1 and Liver
 Kidney Antibodies  >9 publications with RFC1 and Kidney
 Intestine Antibodies  >8 publications with RFC1 and Intestine
 Brain Antibodies  >8 publications with RFC1 and Brain
 Lung Antibodies  >7 publications with RFC1 and Lung
 Bone Antibodies  >5 publications with RFC1 and Bone
 Stomach Antibodies  >4 publications with RFC1 and Stomach
 Vascular Antibodies  >4 publications with RFC1 and Vascular
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