Rabbit Anti-IL17A Polyclonal Antibody – Cat# MBS175185

Figure. Western blot analysis of IL-17 using anti- IL-17 antibody (MBS175185).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane : Recombinant Mouse IL-17 Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-17 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.

IL17A Antibody – Rabbit Polyclonal

Name: IL17A Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS175185
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175185

Description: Rabbit IgG polyclonal antibody for Interleukin-17A(IL17A) detection. Tested with WB, ELISA in Mouse.Background: IL-17(Interleukin-17), originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al. in 1993, is the founding… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL17 (IL17A), Polyclonal Antibody

Full Product Name

Anti-mouse IL17 antibody

Product Gene Name

anti-IL17A antibody

Product Synonym Names

Interleukin-17A(IL-17/IL-17A); interleukin 17A;Cytotoxic T-lymphocyte-associated antigen 8;CTLA-8; CTLA 8 antibody; CTLA-8 antibody; CTLA8 antibody; Cytotoxic T lymphocyte associated antigen 8 antibody; Cytotoxic T lymphocyte associated protein 8 antibody; Cytotoxic T lymphocyte associated serine esterase 8 antibody; Cytotoxic T-lymphocyte-associated antigen 8 antibody; IL 17A antibody; IL-17 antibody; IL-17A antibody; IL17 antibody; IL17A antibody; Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody; Interleukin 17A antibody; Interleukin-17A antibody; Interleukin17 antibodyInter; leukin17A antibody; OTTHUMP00000016597 antibody; OTTMUSP00000046003 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

Lyophilized

Purity/Purification

Immunogen affinity purified.

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

17,490 Da

3D Structure

ModBase 3D Structure for Q62386

Immunogen

E Coli-derived mouse IL-17 recombinant protein (Position: A26-A158).

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-IL17A antibody

Western Blot (WB), ELISA (EIA)

Other Notes

Small volumes of anti-IL17A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL17A purchase

MBS175185 is an antibody targeting IL17A. Also known as Anti-mouse IL17 antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL17A. It may not necessarily be applicable to this product.

NCBI GI #

2498482

NCBI GeneID

16171

NCBI Accession #

Q62386.1

UniProt Primary Accession #

Q62386

UniProt Secondary Accession #

Q60971

UniProt Related Accession #

Q62386

NCBI Official Full Name

Interleukin-17A

NCBI Official Synonym Full Names

interleukin 17A

NCBI Official Symbol

Il17a

NCBI Official Synonym Symbols

Il17; Ctla8; IL-17; Ctla-8; IL-17A

UniProt Protein Name

Interleukin-17A

UniProt Synonym Protein Names

Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

Protein Family

Interleukin

UniProt Gene Name

Il17a

UniProt Synonym Gene Names

Ctla8; Il17; IL-17; IL-17A; CTLA-8

UniProt Entry Name

IL17_MOUSE

NCBI Protein Information

interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8

UniProt Comments for IL17A

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Research Articles on IL17A

1. Results show an opposing relationship between Roquin-1 and the IL-17a proinflammatory cytokine. Enforced expression of Rc3h1 restricts Il17a expression, and exposure of T cells to IL-10 increases Rc3h1 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL17A antibody

Reference Product	PubMed Publications
Foxp3 antibody	>639 publications with IL17A and Foxp3
Stat3 antibody	>274 publications with IL17A and Stat3
Il17ra antibody	>111 publications with IL17A and Il17ra
Stat1 antibody	>86 publications with IL17A and Stat1
Il23a antibody	>65 publications with IL17A and Il23a
Icam1 antibody	>55 publications with IL17A and Icam1
Il17rc antibody	>25 publications with IL17A and Il17rc
Il17rb antibody	>19 publications with IL17A and Il17rb

Pathways associated with anti-IL17A antibody

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-IL17A antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2382 publications with IL17A and Inflammation
Autoimmune Diseases Antibodies	>1819 publications with IL17A and Autoimmune Diseases
Disease Models, Animal Antibodies	>1299 publications with IL17A and Disease Models, Animal
Necrosis Antibodies	>989 publications with IL17A and Necrosis
Respiratory Tract Diseases Antibodies	>690 publications with IL17A and Respiratory Tract Diseases
Lung Diseases Antibodies	>608 publications with IL17A and Lung Diseases
Central Nervous System Diseases Antibodies	>536 publications with IL17A and Central Nervous System Diseases
Hypersensitivity Antibodies	>492 publications with IL17A and Hypersensitivity
Encephalomyelitis, Autoimmune, Experimental Antibodies	>459 publications with IL17A and Encephalomyelitis, Autoimmune, Experimental
Liver Diseases Antibodies	>187 publications with IL17A and Liver Diseases

Organs/Tissues associated with anti-IL17A antibody

Organ/Tissue Name	Pubmed Publications
Thymus Antibodies	>103 publications with IL17A and Thymus