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CXCR3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751935

Rabbit IgG Monoclonal antibody for P49682 detection. Tested positive for WB IF/IF IP in Human More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CXCR3, Monoclonal Antibody
Full Product Name
Anti-CXCR3 Monoclonal Antibody
Product Gene Name
anti-CXCR3 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
45,523 Da
3D Structure
ModBase 3D Structure for P49682
Immunogen
A synthesized peptide derived from human CXCR3 Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CXCR3 antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CXCR3 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-CXCR3 antibody

Figure 1. Western blot analysis of CXCR3 using anti-CXCR3 antibody (M00295).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCR3.
Rabbit Anti-CXCR3 Monoclonal Antibody – Cat# MBS1751935

Other Notes

Small volumes of anti-CXCR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CXCR3 purchase

MBS1751935 is an antibody targeting CXCR3. Also known as Anti-CXCR3 Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCR3. It may not necessarily be applicable to this product.

NCBI GI #
218563730
NCBI GeneID
2833
NCBI Accession #
NP_001136269.1
UniProt Primary Accession #
P49682
UniProt Secondary Accession #
O15185; Q7Z710; Q9P2T4; Q9P2T5; B2R982
UniProt Related Accession #
P49682
GenBank Nucleotide #
NM_001142797.1
NCBI Official Full Name
C-X-C chemokine receptor type 3 isoform 2
NCBI Official Synonym Full Names
C-X-C motif chemokine receptor 3
NCBI Official Symbol
CXCR3
NCBI Official Synonym Symbols
GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
UniProt Protein Name
C-X-C chemokine receptor type 3
UniProt Synonym Protein Names
CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD_antigen: CD183
Protein Family
C-X-C chemokine receptor
UniProt Gene Name
CXCR3
UniProt Synonym Gene Names
GPR9; CXC-R3; CXCR-3; IP-10 receptor
NCBI Protein Information
C-X-C chemokine receptor type 3

NCBI Summary for CXCR3

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]
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UniProt Comments for CXCR3

Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response.

Research Articles on CXCR3

1. High CXCR3 expression is associated with invasion and metastasis in tongue squamous cell carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CXCR3 antibody

 Reference Product  PubMed Publications
 CXCL10 antibody  >713 publications with CXCR3 and CXCL10
 CXCL9 antibody  >407 publications with CXCR3 and CXCL9
 CXCL11 antibody  >265 publications with CXCR3 and CXCL11
 CXCL13 antibody  >25 publications with CXCR3 and CXCL13
 PF4 antibody  >14 publications with CXCR3 and PF4

Pathways associated with anti-CXCR3 antibody

 Products by Pathway  Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-CXCR3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >494 publications with CXCR3 and Inflammation
 Autoimmune Diseases Antibodies  >272 publications with CXCR3 and Autoimmune Diseases
 Neoplasms Antibodies  >262 publications with CXCR3 and Neoplasms
 Necrosis Antibodies  >176 publications with CXCR3 and Necrosis
 Hypersensitivity Antibodies  >127 publications with CXCR3 and Hypersensitivity
 Liver Diseases Antibodies  >104 publications with CXCR3 and Liver Diseases
 Carcinoma Antibodies  >88 publications with CXCR3 and Carcinoma
 Cardiovascular Diseases Antibodies  >85 publications with CXCR3 and Cardiovascular Diseases
 Fibrosis Antibodies  >65 publications with CXCR3 and Fibrosis
 Kidney Diseases Antibodies  >58 publications with CXCR3 and Kidney Diseases

Organs/Tissues associated with anti-CXCR3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1290 publications with CXCR3 and Blood
 Brain Antibodies  >122 publications with CXCR3 and Brain
 Spleen Antibodies  >105 publications with CXCR3 and Spleen
 Intestine Antibodies  >58 publications with CXCR3 and Intestine
 Muscle Antibodies  >47 publications with CXCR3 and Muscle
 Connective Tissue Antibodies  >12 publications with CXCR3 and Connective Tissue
 Uncharacterized Tissue Antibodies  >1 publications with CXCR3 and Uncharacterized Tissue
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