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SF2 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751968

Rabbit IgG Monoclonal antibody for Q07955 detection. Tested positive for WB IHC IF/IF FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SF2, Monoclonal Antibody
Full Product Name
Anti-SF2 Monoclonal Antibody
Product Gene Name
anti-SF2 antibody
Product Synonym Gene Name
SRSF1
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
31,999 Da
3D Structure
ModBase 3D Structure for Q07955
Immunogen
A synthesized peptide derived from human SF2 Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SF2 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SF2 antibody
WB: 1:500-1:2000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-SF2 antibody

Figure 1. Western blot analysis of SRSF1 using anti-SRSF1 antibody (M00497).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRSF1.
Rabbit Anti-SF2 Monoclonal Antibody – Cat# MBS1751968

Other Notes

Small volumes of anti-SF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF2 purchase

MBS1751968 is an antibody targeting SF2. Also known as Anti-SF2 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SF2. It may not necessarily be applicable to this product.

NCBI GI #
118582269
NCBI GeneID
6426
NCBI Accession #
NP_001071634.1
UniProt Primary Accession #
Q07955
UniProt Secondary Accession #
Q13809; B2R6Z7; D3DTZ3
UniProt Related Accession #
Q07955
GenBank Nucleotide #
NM_001078166.1
NCBI Official Full Name
serine/arginine-rich splicing factor 1 isoform 2
NCBI Official Synonym Full Names
serine and arginine rich splicing factor 1
NCBI Official Symbol
SRSF1
NCBI Official Synonym Symbols
ASF; SF2; SFRS1; SF2p33; SRp30a
UniProt Protein Name
Serine/arginine-rich splicing factor 1
UniProt Synonym Protein Names
Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit
Protein Family
Serine/arginine-rich splicing factor
UniProt Gene Name
SRSF1
UniProt Synonym Gene Names
ASF; SF2; SF2P33; SFRS1; ASF-1
NCBI Protein Information
serine/arginine-rich splicing factor 1

NCBI Summary for SF2

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]
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UniProt Comments for SF2

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
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Research Articles on SF2

1. A -44 G to A "hot zone" putative functional noncoding variant of SRSF1 was found in an AML patient. It alters the binding activities of E2F6, ELF1, and ELK4, ELK4.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SF2 antibody

 Reference Product  PubMed Publications
 SRSF2 antibody  >8 publications with SF2 and SRSF2
 SRPK1 antibody  >8 publications with SF2 and SRPK1
 SRSF4 antibody  >1 publications with SF2 and SRSF4

Pathways associated with anti-SF2 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram

Diseases associated with anti-SF2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with SF2 and Neoplasms
 Carcinoma Antibodies  >8 publications with SF2 and Carcinoma
 Lung Neoplasms Antibodies  >4 publications with SF2 and Lung Neoplasms
 Adenocarcinoma Antibodies  >4 publications with SF2 and Adenocarcinoma
 Kidney Diseases Antibodies  >3 publications with SF2 and Kidney Diseases
 Kidney Neoplasms Antibodies  >2 publications with SF2 and Kidney Neoplasms
 Cardiovascular Diseases Antibodies  >2 publications with SF2 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >2 publications with SF2 and Breast Neoplasms
 Skin Diseases Antibodies  >2 publications with SF2 and Skin Diseases
 Neoplasms, Experimental Antibodies  >1 publications with SF2 and Neoplasms, Experimental

Organs/Tissues associated with anti-SF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >6 publications with SF2 and Lung
 Blood Antibodies  >5 publications with SF2 and Blood
 Vascular Antibodies  >4 publications with SF2 and Vascular
 Heart Antibodies  >3 publications with SF2 and Heart
 Brain Antibodies  >3 publications with SF2 and Brain
 Eye Antibodies  >2 publications with SF2 and Eye
 Liver Antibodies  >2 publications with SF2 and Liver
 Kidney Antibodies  >2 publications with SF2 and Kidney
 Ovary Antibodies  >2 publications with SF2 and Ovary
 Mammary Gland Antibodies  >1 publications with SF2 and Mammary Gland
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