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PON1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751975

Rabbit IgG Monoclonal antibody for P27169 detection. Tested positive for WB IP in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PON1, Monoclonal Antibody
Full Product Name
Anti-PON1 Monoclonal Antibody
Product Gene Name
anti-PON1 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
39,731 Da
3D Structure
ModBase 3D Structure for P27169
Immunogen
A synthesized peptide derived from human PON1 Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PON1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PON1 antibody
WB: 1:1000-1:5000
IP: 1:50

Western Blot (WB) of anti-PON1 antibody

Figure 1. Western blot analysis of PON1 using anti-PON1 antibody (M00516-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PON1.
Rabbit Anti-PON1 Monoclonal Antibody – Cat# MBS1751975

Other Notes

Small volumes of anti-PON1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PON1 purchase

MBS1751975 is an antibody targeting PON1. Also known as Anti-PON1 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PON1. It may not necessarily be applicable to this product.

NCBI GI #
19923106
NCBI GeneID
5444
NCBI Accession #
NP_000437.3
UniProt Primary Accession #
P27169
UniProt Secondary Accession #
Q16052; Q6B0J6; Q9UCB1; B2RA40
UniProt Related Accession #
P27169
GenBank Nucleotide #
NM_000446.6
NCBI Official Full Name
serum paraoxonase/arylesterase 1
NCBI Official Synonym Full Names
paraoxonase 1
NCBI Official Symbol
PON1
NCBI Official Synonym Symbols
ESA; PON; MVCD5
UniProt Protein Name
Serum paraoxonase/arylesterase 1
UniProt Synonym Protein Names
Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1
Protein Family
Serum paraoxonase/arylesterase
UniProt Gene Name
PON1
UniProt Synonym Gene Names
PON; PON 11 PublicationManual assertion based on opinion iniRef.20; A-esterase 1
NCBI Protein Information
serum paraoxonase/arylesterase 1

NCBI Summary for PON1

This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017]
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UniProt Comments for PON1

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
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Research Articles on PON1

1. Low serum paraoxonase 1 activity is associated with the development of type 2 diabetes mellitus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PON1 antibody

 Reference Product  PubMed Publications
 APOA1 antibody  >25 publications with PON1 and APOA1
 PPARG antibody  >13 publications with PON1 and PPARG
 CLU antibody  >10 publications with PON1 and CLU
 PLA2G7 antibody  >4 publications with PON1 and PLA2G7
 LPA antibody  >2 publications with PON1 and LPA
 SREBF2 antibody  >2 publications with PON1 and SREBF2
 APOA2 antibody  >2 publications with PON1 and APOA2

Pathways associated with anti-PON1 antibody

 Products by Pathway  Pathway Diagram
 Phase I, Non P450 Pathway antibodies  Phase I, Non P450 Pathway Diagram

Diseases associated with anti-PON1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >561 publications with PON1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >441 publications with PON1 and Atherosclerosis
 Coronary Artery Disease Antibodies  >262 publications with PON1 and Coronary Artery Disease
 Coronary Disease Antibodies  >261 publications with PON1 and Coronary Disease
 Heart Diseases Antibodies  >222 publications with PON1 and Heart Diseases
 Diabetes Mellitus Antibodies  >147 publications with PON1 and Diabetes Mellitus
 Inflammation Antibodies  >117 publications with PON1 and Inflammation
 Kidney Diseases Antibodies  >74 publications with PON1 and Kidney Diseases
 Poisoning Antibodies  >74 publications with PON1 and Poisoning
 Drug Toxicity Antibodies  >67 publications with PON1 and Drug Toxicity

Organs/Tissues associated with anti-PON1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >168 publications with PON1 and Liver
 Brain Antibodies  >68 publications with PON1 and Brain
 Lung Antibodies  >42 publications with PON1 and Lung
 Bone Antibodies  >10 publications with PON1 and Bone
 Pancreas Antibodies  >8 publications with PON1 and Pancreas
 Testis Antibodies  >3 publications with PON1 and Testis
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