Rabbit Anti-CFH Monoclonal Antibody – Cat# MBS1751982

Figure 1. Western blot analysis of CFH using anti-CFH antibody (M00562).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFH antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CFH.

Factor H (CFH) Antibody – Rabbit Monoclonal

Name: CFH Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1751982
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751982

Rabbit IgG Monoclonal antibody for P08603 detection. Tested positive for WB IF in Human More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Factor H (CFH), Monoclonal Antibody

Full Product Name

Anti-Factor H Monoclonal Antibody

Product Gene Name

anti-CFH antibody

Clonality

Monoclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-Chromatography

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.

Molecular Weight

51,034 Da

3D Structure

ModBase 3D Structure for P08603

Immunogen

A synthesized peptide derived from human Factor H Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CFH antibody

Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)

Application Notes for anti-CFH antibody

WB: 1:500-1:2000
ICC/IF: 1:50-1:200

Other Notes

Small volumes of anti-CFH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFH purchase

MBS1751982 is an antibody targeting CFH. Also known as Anti-Factor H Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CFH. It may not necessarily be applicable to this product.

NCBI GI #

62739186

NCBI GeneID

3075

NCBI Accession #

NP_000177.2

UniProt Primary Accession #

P08603

UniProt Secondary Accession #

P78435; Q14570; Q2TAZ5; Q38G77; Q5TFM3; Q8N708; Q9NU86; A5PL14

UniProt Related Accession #

P08603

GenBank Nucleotide #

NM_000186.3

NCBI Official Full Name

complement factor H isoform a

NCBI Official Synonym Full Names

complement factor H

NCBI Official Symbol

CFH

NCBI Official Synonym Symbols

FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3

UniProt Protein Name

Complement factor H

UniProt Synonym Protein Names

H factor 1

Protein Family

Complement factor

UniProt Gene Name

CFH

UniProt Synonym Gene Names

HF; HF1; HF2

NCBI Protein Information

complement factor H

NCBI Summary for CFH

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]

UniProt Comments for CFH

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.CautionAccording to a report, Asn-217 is not glycosylated (PubMed:17591618). Another study observed glycosylation at this position (PubMed:19139490).

Research Articles on CFH

1. These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CFH antibody

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement Cascade Pathway antibodies	Complement Cascade Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Regulation Of Complement Cascade Pathway antibodies	Regulation Of Complement Cascade Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram

Diseases associated with anti-CFH antibody

Disease Name	Pubmed Publications
Macular Degeneration Antibodies	>406 publications with CFH and Macular Degeneration
Macular Degeneration, Age-Related, 4 Antibodies	>161 publications with CFH and Macular Degeneration, Age-Related, 4
Kidney Diseases Antibodies	>131 publications with CFH and Kidney Diseases
Atypical hemolytic uremic syndrome Antibodies	>87 publications with CFH and Atypical hemolytic uremic syndrome
Inflammation Antibodies	>68 publications with CFH and Inflammation
Cardiovascular Diseases Antibodies	>57 publications with CFH and Cardiovascular Diseases
Atrophy Antibodies	>46 publications with CFH and Atrophy
Glomerulonephritis, Membranoproliferative Antibodies	>36 publications with CFH and Glomerulonephritis, Membranoproliferative
Mental Disorders Antibodies	>24 publications with CFH and Mental Disorders
Hypertension Antibodies	>24 publications with CFH and Hypertension

Organs/Tissues associated with anti-CFH antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>202 publications with CFH and Eye
Kidney Antibodies	>99 publications with CFH and Kidney
Vascular Antibodies	>66 publications with CFH and Vascular
Ear Antibodies	>37 publications with CFH and Ear
Brain Antibodies	>32 publications with CFH and Brain
Liver Antibodies	>28 publications with CFH and Liver
Heart Antibodies	>24 publications with CFH and Heart
Nerve Antibodies	>13 publications with CFH and Nerve
Skin Antibodies	>11 publications with CFH and Skin
Bone Antibodies	>7 publications with CFH and Bone