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CASR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751988

Description: Mouse IgG monoclonal antibody for CASR detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.Background: The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief… More details

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Product Overview

Online Datasheet in PDF
Product Name
CASR, Monoclonal Antibody
Full Product Name
Anti-CASR Antibody (monoclonal, 11E9)
Product Gene Name
anti-CASR antibody
Product Synonym Names
Extracellular calcium-sensing receptor; CaR; Calcium sensing receptor
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11E9
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
121,773 Da
3D Structure
ModBase 3D Structure for P41180
Immunogen
E Coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P, IHC-F and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CASR antibody
Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-CASR antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
IHC-F: 0.5-1 mug/ml
ICC: 0.5-1 mug/ml
FC/FACS: 1-3ug/1x106 cells
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Flow Cytometry (FC/FACS) of anti-CASR antibody

Figure 1. Flow Cytometry analysis of NEURO-2A cells using anti-CASR antibody (M00574).
Overlay histogram showing NEURO-2A cells stained with M00574 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CASR Antibody (M00574, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Mouse Anti-CASR Monoclonal Antibody – Cat# MBS1751988

Product References and Citations for anti-CASR antibody

1. Coburn JW, Elangovan L, Goodman WG, Frazaõ JM (Dec 1999). "Calcium-sensing receptor and calcimimetic agents". Kidney International. Supplement 73: S52-8. 2. D'Souza-Li L (Aug 2006). "The calcium-sensing receptor and related diseases". Arquivos Brasileiros De Endocrinologia E Metabologia 50 (4): 628-39. 3. Pidasheva S, Canaff L, Simonds WF, Marx SJ, Hendy GN (Jun 2005). "Impaired cotranslational processing of the calcium-sensing receptor due to signal peptide missense mutations in familial hypocalciuric hypercalcemia". Human Molecular Genetics 14 (12): 1679-90.

Other Notes

Small volumes of anti-CASR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CASR purchase

MBS1751988 is an antibody targeting CASR. Also known as Anti-CASR Antibody (monoclonal, 11E9). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CASR. It may not necessarily be applicable to this product.

NCBI GI #
37577159
NCBI GeneID
846
NCBI Accession #
NP_000379.2
UniProt Primary Accession #
P41180
UniProt Secondary Accession #
Q13912; Q16108; Q16109; Q16110; Q16379; Q2M1T0; Q4PJ19
UniProt Related Accession #
P41180
GenBank Nucleotide #
NM_000388.3
NCBI Official Full Name
extracellular calcium-sensing receptor isoform 2
NCBI Official Synonym Full Names
calcium sensing receptor
NCBI Official Symbol
CASR
NCBI Official Synonym Symbols
CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1
UniProt Protein Name
Extracellular calcium-sensing receptor
UniProt Synonym Protein Names
Parathyroid cell calcium-sensing receptor 11 PublicationManual assertion based on opinion iniRef.19"The Ca(2+)-sensing receptor gene (PCAR1) mutation T151M in isolated autosomal dominant hypoparathyroidism."Lovlie R., Eiken H.G., Sorheim J.I., Boman H.Hum. Genet. 98:129-133(1996) [PubMed] [Europe PMC] [Abstract]Cited for: VARIANT FIH MET-151.
Protein Family
Extracellular calcium-sensing receptor
UniProt Gene Name
CASR
UniProt Synonym Gene Names
CaSR; hCasR1 PublicationManual assertion based on opinion iniRef.14; PCaR11 PublicationManual assertion based on opinion iniRef.19
NCBI Protein Information
extracellular calcium-sensing receptor

NCBI Summary for CASR

The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]
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UniProt Comments for CASR

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed:7759551, PubMed:8702647, PubMed:8636323, PubMed:8878438, PubMed:17555508, PubMed:19789209, PubMed:21566075, PubMed:22114145, PubMed:23966241, PubMed:25292184, PubMed:25104082, PubMed:26386835, PubMed:25766501, PubMed:22789683). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed:27434672, PubMed:27386547).
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Research Articles on CASR

1. These findings suggest an inhibitory role for CaSR in endometrial cancer. Therefore, reduced CaSR expression may be a suitable explanation and valuable predictor for endometrial cancer progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CASR antibody

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 Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway antibodies  Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway Diagram
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 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway antibodies  GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-CASR antibody

 Disease Name  Pubmed Publications
 Hyperparathyroidism Antibodies  >195 publications with CASR and Hyperparathyroidism
 Hypercalcemia Antibodies  >165 publications with CASR and Hypercalcemia
 Hypocalcemia Antibodies  >97 publications with CASR and Hypocalcemia
 Neoplasms Antibodies  >96 publications with CASR and Neoplasms
 Kidney Diseases Antibodies  >83 publications with CASR and Kidney Diseases
 Hyperparathyroidism, Secondary Antibodies  >66 publications with CASR and Hyperparathyroidism, Secondary
 Hypoparathyroidism Antibodies  >59 publications with CASR and Hypoparathyroidism
 Hypertension Antibodies  >29 publications with CASR and Hypertension
 Hyperparathyroidism, Neonatal Severe Primary Antibodies  >21 publications with CASR and Hyperparathyroidism, Neonatal Severe Primary
 Renal Insufficiency Antibodies  >19 publications with CASR and Renal Insufficiency

Organs/Tissues associated with anti-CASR antibody

 Organ/Tissue Name  Pubmed Publications
 Parathyroid Antibodies  >326 publications with CASR and Parathyroid
 Kidney Antibodies  >195 publications with CASR and Kidney
 Liver Antibodies  >28 publications with CASR and Liver
 Pancreas Antibodies  >12 publications with CASR and Pancreas
 Eye Antibodies  >1 publications with CASR and Eye
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