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Emerin (EMD) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752009

Rabbit IgG Monoclonal antibody for P50402 detection. Tested positive for WB IHC IF/IF in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Emerin (EMD), Monoclonal Antibody
Full Product Name
Anti-Emerin Monoclonal Antibody
Product Gene Name
anti-EMD antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
28,994 Da
3D Structure
ModBase 3D Structure for P50402
Immunogen
A synthesized peptide derived from human Emerin Emerin may regulate gene expression through binding to other transcriptional regulators. Emerin binds to Beta -catenin and inhibits its nuclear accumulation. Recent studies demonstrate that Emerin is required for HIV-1 infectivity.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EMD antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EMD antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-EMD antibody

Figure 1. Western blot analysis of EMD using anti-EMD antibody (M00714-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EMD antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EMD.
Rabbit Anti-EMD Monoclonal Antibody – Cat# MBS1752009

Other Notes

Small volumes of anti-EMD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EMD purchase

MBS1752009 is an antibody targeting EMD. Also known as Anti-Emerin Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EMD. It may not necessarily be applicable to this product.

NCBI GI #
4557553
NCBI GeneID
2010
NCBI Accession #
NP_000108.1
UniProt Primary Accession #
P50402
UniProt Secondary Accession #
Q6FI02
UniProt Related Accession #
P50402
GenBank Nucleotide #
NM_000117.2
NCBI Official Full Name
emerin
NCBI Official Synonym Full Names
emerin
NCBI Official Symbol
EMD
NCBI Official Synonym Symbols
STA; EDMD; LEMD5
UniProt Protein Name
Emerin
Protein Family
Emerin
UniProt Gene Name
EMD
UniProt Synonym Gene Names
EDMD; STA
NCBI Protein Information
emerin

NCBI Summary for EMD

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for EMD

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
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Research Articles on EMD

1. Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient in Emery-Dreifuss muscular dystrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EMD antibody

 Reference Product  PubMed Publications
 LMNA antibody  >17 publications with EMD and LMNA
 SYNE2 antibody  >2 publications with EMD and SYNE2
 SRC antibody  >2 publications with EMD and SRC
 SYNE1 antibody  >2 publications with EMD and SYNE1
 ERBB2 antibody  >1 publications with EMD and ERBB2
 ACTB antibody  >1 publications with EMD and ACTB

Pathways associated with anti-EMD antibody

 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Clearance Of Nuclear Envelope Membranes From Chromatin Pathway antibodies  Clearance Of Nuclear Envelope Membranes From Chromatin Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram

Diseases associated with anti-EMD antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >223 publications with EMD and Heart Diseases
 Musculoskeletal Diseases Antibodies  >202 publications with EMD and Musculoskeletal Diseases
 Nervous System Diseases Antibodies  >141 publications with EMD and Nervous System Diseases
 Neoplasms Antibodies  >128 publications with EMD and Neoplasms
 Muscular Diseases Antibodies  >51 publications with EMD and Muscular Diseases
 Drug Toxicity Antibodies  >46 publications with EMD and Drug Toxicity
 Muscular Dystrophy, Emery-Dreifuss Antibodies  >44 publications with EMD and Muscular Dystrophy, Emery-Dreifuss
 Death Antibodies  >39 publications with EMD and Death
 Inflammation Antibodies  >34 publications with EMD and Inflammation
 Hemorrhage Antibodies  >28 publications with EMD and Hemorrhage

Organs/Tissues associated with anti-EMD antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >346 publications with EMD and Heart
 Bone Antibodies  >333 publications with EMD and Bone
 Muscle Antibodies  >300 publications with EMD and Muscle
 Blood Antibodies  >245 publications with EMD and Blood
 Brain Antibodies  >143 publications with EMD and Brain
 Connective Tissue Antibodies  >91 publications with EMD and Connective Tissue
 Vascular Antibodies  >82 publications with EMD and Vascular
 Eye Antibodies  >61 publications with EMD and Eye
 Skin Antibodies  >50 publications with EMD and Skin
 Liver Antibodies  >49 publications with EMD and Liver
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