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EEF2 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752024

Rabbit IgG Monoclonal antibody for P13639 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EEF2, Monoclonal Antibody
Full Product Name
Anti-EEF2 Monoclonal Antibody
Product Gene Name
anti-EEF2 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
95,338 Da
3D Structure
ModBase 3D Structure for P13639
Immunogen
A synthesized peptide derived from human EEF2 Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EEF2 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EEF2 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:20
FC/FACS: 1:50

Western Blot (WB) of anti-EEF2 antibody

Figure 1. Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEF2.
Rabbit Anti-EEF2 Monoclonal Antibody – Cat# MBS1752024

Other Notes

Small volumes of anti-EEF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EEF2 purchase

MBS1752024 is an antibody targeting EEF2. Also known as Anti-EEF2 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF2. It may not necessarily be applicable to this product.

NCBI GI #
4503483
NCBI GeneID
1938
NCBI Accession #
NP_001952.1
UniProt Primary Accession #
P13639
UniProt Secondary Accession #
Q58J86; B2RMP5; D6W618
UniProt Related Accession #
P13639
GenBank Nucleotide #
NM_001961.4
NCBI Official Full Name
elongation factor 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 2
NCBI Official Symbol
EEF2
NCBI Official Synonym Symbols
EF2; EF-2; EEF-2; SCA26
UniProt Protein Name
Elongation factor 2
Protein Family
Elongation factor
UniProt Gene Name
EEF2
UniProt Synonym Gene Names
EF2; EF-2
NCBI Protein Information
elongation factor 2

NCBI Summary for EEF2

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
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UniProt Comments for EEF2

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
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Research Articles on EEF2

1. our study indicates that expression of eEF2 protein is a potential biomarker for evaluating prostate cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EEF2 antibody

 Reference Product  PubMed Publications
 TRPM7 antibody  >2 publications with EEF2 and TRPM7
 MAPKAPK3 antibody  >1 publications with EEF2 and MAPKAPK3

Pathways associated with anti-EEF2 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Chain Elongation Pathway antibodies  Peptide Chain Elongation Pathway Diagram
 Translation Pathway antibodies  Translation Pathway Diagram
 Translation Factors Pathway antibodies  Translation Factors Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-EEF2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >18 publications with EEF2 and Neoplasms
 Hypertrophy Antibodies  >17 publications with EEF2 and Hypertrophy
 Carcinoma Antibodies  >10 publications with EEF2 and Carcinoma
 Cardiovascular Diseases Antibodies  >10 publications with EEF2 and Cardiovascular Diseases
 Necrosis Antibodies  >6 publications with EEF2 and Necrosis
 Heart Diseases Antibodies  >6 publications with EEF2 and Heart Diseases
 Adenocarcinoma Antibodies  >6 publications with EEF2 and Adenocarcinoma
 Inflammation Antibodies  >5 publications with EEF2 and Inflammation
 Brain Diseases Antibodies  >5 publications with EEF2 and Brain Diseases
 Drug Toxicity Antibodies  >5 publications with EEF2 and Drug Toxicity

Organs/Tissues associated with anti-EEF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >83 publications with EEF2 and Muscle
 Brain Antibodies  >32 publications with EEF2 and Brain
 Blood Antibodies  >30 publications with EEF2 and Blood
 Heart Antibodies  >24 publications with EEF2 and Heart
 Liver Antibodies  >15 publications with EEF2 and Liver
 Kidney Antibodies  >12 publications with EEF2 and Kidney
 Vascular Antibodies  >8 publications with EEF2 and Vascular
 Pancreas Antibodies  >7 publications with EEF2 and Pancreas
 Lung Antibodies  >4 publications with EEF2 and Lung
 Bone Antibodies  >4 publications with EEF2 and Bone
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