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TXNIP Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752096

Rabbit IgG Monoclonal antibody for Q9H3M7 detection. Tested positive for WB IHC IF/IF FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TXNIP, Monoclonal Antibody
Full Product Name
Anti-TXNIP Monoclonal Antibody
Product Gene Name
anti-TXNIP antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
37,319 Da
3D Structure
ModBase 3D Structure for Q9H3M7
Immunogen
A synthesized peptide derived from human TXNIP May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TXNIP antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TXNIP antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:100

Western Blot (WB) of anti-TXNIP antibody

Figure 1. Western blot analysis of TXNIP using anti-TXNIP antibody (M01409).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNIP antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TXNIP.
Rabbit Anti-TXNIP Monoclonal Antibody – Cat# MBS1752096

Other Notes

Small volumes of anti-TXNIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TXNIP purchase

MBS1752096 is an antibody targeting TXNIP. Also known as Anti-TXNIP Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TXNIP. It may not necessarily be applicable to this product.

NCBI GI #
928192549
NCBI GeneID
10628
NCBI Accession #
NP_001300901.1
UniProt Primary Accession #
Q9H3M7
UniProt Secondary Accession #
Q16226; Q6PML0; Q9BXG9; B4E3D3
UniProt Related Accession #
Q9H3M7
GenBank Nucleotide #
NM_001313972.1
NCBI Official Full Name
thioredoxin-interacting protein isoform 2
NCBI Official Synonym Full Names
thioredoxin interacting protein
NCBI Official Symbol
TXNIP
NCBI Official Synonym Symbols
THIF; VDUP1; ARRDC6; HHCPA78; EST01027
UniProt Protein Name
Thioredoxin-interacting protein
UniProt Synonym Protein Names
Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1
Protein Family
Thioredoxin-interacting protein
UniProt Gene Name
TXNIP
UniProt Synonym Gene Names
VDUP1
NCBI Protein Information
thioredoxin-interacting protein

NCBI Summary for TXNIP

This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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UniProt Comments for TXNIP

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
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Research Articles on TXNIP

1. Using oxygen-glucose deprivation and reoxygenation (OGD/R) to create a cell model of hepatic I/R injury, we found that the mRNA and protein expression levels of TXNIP were upregulated in HL7702cells exposed to OGD/R.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TXNIP antibody

 Reference Product  PubMed Publications
 TXN antibody  >20 publications with TXNIP and TXN
 MAP3K5 antibody  >11 publications with TXNIP and MAP3K5
 FOXO1 antibody  >7 publications with TXNIP and FOXO1
 NOS3 antibody  >3 publications with TXNIP and NOS3
 FOXO3 antibody  >2 publications with TXNIP and FOXO3
 IL2RB antibody  >1 publications with TXNIP and IL2RB
 COPS5 antibody  >1 publications with TXNIP and COPS5

Pathways associated with anti-TXNIP antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 NOD-like Receptor Signaling Pathway antibodies  NOD-like Receptor Signaling Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram
 The NLRP3 Inflammasome Pathway antibodies  The NLRP3 Inflammasome Pathway Diagram

Diseases associated with anti-TXNIP antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >114 publications with TXNIP and Inflammation
 Cardiovascular Diseases Antibodies  >76 publications with TXNIP and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >66 publications with TXNIP and Disease Models, Animal
 Necrosis Antibodies  >30 publications with TXNIP and Necrosis
 Insulin Resistance Antibodies  >30 publications with TXNIP and Insulin Resistance
 Heart Diseases Antibodies  >29 publications with TXNIP and Heart Diseases
 Liver Diseases Antibodies  >27 publications with TXNIP and Liver Diseases
 Kidney Diseases Antibodies  >26 publications with TXNIP and Kidney Diseases
 Adenocarcinoma Antibodies  >25 publications with TXNIP and Adenocarcinoma
 Breast Neoplasms Antibodies  >23 publications with TXNIP and Breast Neoplasms

Organs/Tissues associated with anti-TXNIP antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >113 publications with TXNIP and Blood
 Liver Antibodies  >62 publications with TXNIP and Liver
 Vascular Antibodies  >61 publications with TXNIP and Vascular
 Kidney Antibodies  >51 publications with TXNIP and Kidney
 Muscle Antibodies  >50 publications with TXNIP and Muscle
 Heart Antibodies  >49 publications with TXNIP and Heart
 Pancreas Antibodies  >45 publications with TXNIP and Pancreas
 Brain Antibodies  >38 publications with TXNIP and Brain
 Lung Antibodies  >29 publications with TXNIP and Lung
 Eye Antibodies  >24 publications with TXNIP and Eye
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