Rabbit Anti-FSHB Monoclonal Antibody – Cat# MBS1752144

Figure 1. Western blot analysis of FSHB using anti-FSHB antibody (M01885-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSHB antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FSHB.

FSH Beta (FSHB) Antibody – Rabbit Monoclonal

Name: FSHB Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752144
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752144

Rabbit IgG Monoclonal antibody for P01225 detection. Tested positive for WB IP in Human Rabbit More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FSH beta (FSHB), Monoclonal Antibody

Full Product Name

Anti-FSH beta (FSHB) Monoclonal Antibody

Product Gene Name

anti-FSHB antibody

Clonality

Monoclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-Chromatography

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.

Molecular Weight

14,700 Da

3D Structure

ModBase 3D Structure for P01225

Immunogen

A synthesized peptide derived from human FSH beta (FSHB) Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-FSHB antibody

Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-FSHB antibody

WB: 1:500-1:2000
IP: 1:50

Other Notes

Small volumes of anti-FSHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FSHB purchase

MBS1752144 is an antibody targeting FSHB. Also known as Anti-FSH beta (FSHB) Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FSHB. It may not necessarily be applicable to this product.

NCBI GI #

4503791

NCBI GeneID

2488

NCBI Accession #

NP_000501.1

UniProt Primary Accession #

P01225

UniProt Secondary Accession #

Q14D61; A2TF08; A5JVV3

UniProt Related Accession #

P01225

GenBank Nucleotide #

NM_000510.2

NCBI Official Full Name

follitropin subunit beta

NCBI Official Synonym Full Names

follicle stimulating hormone subunit beta

NCBI Official Symbol

FSHB

NCBI Official Synonym Symbols

HH24

UniProt Protein Name

Follitropin subunit beta

UniProt Synonym Protein Names

Follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; Follitropin beta chain

Protein Family

Follitropin

UniProt Gene Name

FSHB

UniProt Synonym Gene Names

FSH-B; FSH-beta

NCBI Protein Information

follitropin subunit beta

NCBI Summary for FSHB

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

UniProt Comments for FSHB

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways (PubMed:2494176, PubMed:24692546). Follitropin is involved in follicle development and spermatogenesis in reproductive organs (PubMed:407105, PubMed:8220432).

Research Articles on FSHB

1. High follicle stimulating hormone expression is associated with Turner syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FSHB antibody

Reference Product	PubMed Publications
FSHR antibody	>27 publications with FSHB and FSHR
CGA antibody	>27 publications with FSHB and CGA
GNRHR antibody	>16 publications with FSHB and GNRHR
GNRH1 antibody	>7 publications with FSHB and GNRH1
BMP2 antibody	>5 publications with FSHB and BMP2
INHA antibody	>4 publications with FSHB and INHA
INHBB antibody	>3 publications with FSHB and INHBB
INHBA antibody	>1 publications with FSHB and INHBA

Pathways associated with anti-FSHB antibody

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
FSH Signaling Pathway antibodies	FSH Signaling Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Glycoprotein Hormones Pathway antibodies	Glycoprotein Hormones Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram

Diseases associated with anti-FSHB antibody

Disease Name	Pubmed Publications
Infertility, Male Antibodies	>16 publications with FSHB and Infertility, Male
Kidney Diseases Antibodies	>10 publications with FSHB and Kidney Diseases
Infertility, Female Antibodies	>10 publications with FSHB and Infertility, Female
Hypogonadism Antibodies	>6 publications with FSHB and Hypogonadism
Nervous System Diseases Antibodies	>4 publications with FSHB and Nervous System Diseases
Weight Gain Antibodies	>1 publications with FSHB and Weight Gain
Breast Neoplasms Antibodies	>1 publications with FSHB and Breast Neoplasms
Prenatal Exposure Delayed Effects Antibodies	>1 publications with FSHB and Prenatal Exposure Delayed Effects
Follicle-stimulating hormone deficiency, isolated Antibodies	>1 publications with FSHB and Follicle-stimulating hormone deficiency, isolated
Amenorrhea Antibodies	>1 publications with FSHB and Amenorrhea

Organs/Tissues associated with anti-FSHB antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>79 publications with FSHB and Brain
Pituitary Gland Antibodies	>72 publications with FSHB and Pituitary Gland
Testis Antibodies	>24 publications with FSHB and Testis