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DNA Polymerase Beta (POLB) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752152

Rabbit IgG Monoclonal antibody for P06746 detection. Tested positive for WB IHC IF/IF IP in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA Polymerase beta (POLB), Monoclonal Antibody
Full Product Name
Anti-DNA Polymerase beta Monoclonal Antibody
Product Gene Name
anti-POLB antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
38,178 Da
3D Structure
ModBase 3D Structure for P06746
Immunogen
A synthesized peptide derived from human DNA Polymerase beta Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-POLB antibody
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-POLB antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-POLB antibody

Figure 1. Western blot analysis of POLB using anti-POLB antibody (M01946).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLB antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POLB.
Rabbit Anti-POLB Monoclonal Antibody – Cat# MBS1752152

Other Notes

Small volumes of anti-POLB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLB purchase

MBS1752152 is an antibody targeting POLB. Also known as Anti-DNA Polymerase beta Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLB. It may not necessarily be applicable to this product.

NCBI GI #
4505931
NCBI GeneID
5423
NCBI Accession #
NP_002681.1
UniProt Primary Accession #
P06746
UniProt Secondary Accession #
Q3KP48; Q6FI34; B2RC78
UniProt Related Accession #
P06746
GenBank Nucleotide #
NM_002690.3
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
DNA polymerase beta
NCBI Official Symbol
POLB
UniProt Protein Name
DNA polymerase beta
Protein Family
DNA polymerase
UniProt Gene Name
POLB
NCBI Protein Information
DNA polymerase beta

NCBI Summary for POLB

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
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UniProt Comments for POLB

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
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Research Articles on POLB

1. Presently, we describe the preparation of three Pol beta enzymes modified at position 72 with aminooxy or hydrazinyl analogues of lysine. These enzymes form transient covalent bonds with the 5'-dRP moiety of the damaged DNA, in the form of an oxime or hydrazone, respectively

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLB antibody

 Reference Product  PubMed Publications
 XRCC1 antibody  >12 publications with POLB and XRCC1
 PCNA antibody  >10 publications with POLB and PCNA
 APEX1 antibody  >9 publications with POLB and APEX1
 NEIL1 antibody  >3 publications with POLB and NEIL1

Pathways associated with anti-POLB antibody

 Products by Pathway  Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Nucleotide Metabolism Pathway antibodies  Nucleotide Metabolism Pathway Diagram

Diseases associated with anti-POLB antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >29 publications with POLB and Neoplasms
 Carcinoma Antibodies  >9 publications with POLB and Carcinoma
 Drug Toxicity Antibodies  >5 publications with POLB and Drug Toxicity
 Lung Neoplasms Antibodies  >4 publications with POLB and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with POLB and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >3 publications with POLB and Breast Neoplasms
 Urinary Bladder Neoplasms Antibodies  >3 publications with POLB and Urinary Bladder Neoplasms
 Pancreatic Neoplasms Antibodies  >2 publications with POLB and Pancreatic Neoplasms
 Anemia Antibodies  >2 publications with POLB and Anemia
 Hyperplasia Antibodies  >2 publications with POLB and Hyperplasia

Organs/Tissues associated with anti-POLB antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with POLB and Lung
 Liver Antibodies  >3 publications with POLB and Liver
 Blood Antibodies  >3 publications with POLB and Blood
 Kidney Antibodies  >2 publications with POLB and Kidney
 Brain Antibodies  >2 publications with POLB and Brain
 Prostate Antibodies  >1 publications with POLB and Prostate
 Eye Antibodies  >1 publications with POLB and Eye
 Embryonic Tissue Antibodies  >1 publications with POLB and Embryonic Tissue
 Stomach Antibodies  >1 publications with POLB and Stomach
 Testis Antibodies  >1 publications with POLB and Testis
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