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Fascin (FSCN1) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752175

Rabbit IgG Monoclonal antibody for Q16658 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fascin (FSCN1), Monoclonal Antibody
Full Product Name
Anti-Fascin Monoclonal Antibody
Product Gene Name
anti-FSCN1 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
54,530 Da
3D Structure
ModBase 3D Structure for Q16658
Immunogen
A synthesized peptide derived from human Fascin Promotes cross-linkage of parallel actin filaments during the formation of cell protrusions (lamellipodia and filopodia), and therefore plays an important role in regulating cell migration. It has been reported that fascin may also regulate filopodia formation by a mechanism independent of its actin-bundling functions, though less is known about this mechanism of action.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FSCN1 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-FSCN1 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:100

Western Blot (WB) of anti-FSCN1 antibody

Figure 1. Western blot analysis of FSCN1 using anti-FSCN1 antibody (M02147-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FSCN1.
Rabbit Anti-FSCN1 Monoclonal Antibody – Cat# MBS1752175

Other Notes

Small volumes of anti-FSCN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FSCN1 purchase

MBS1752175 is an antibody targeting FSCN1. Also known as Anti-Fascin Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FSCN1. It may not necessarily be applicable to this product.

NCBI GI #
4507115
NCBI GeneID
6624
NCBI Accession #
NP_003079.1
UniProt Primary Accession #
Q16658
UniProt Secondary Accession #
Q96IC5; Q96IH1; Q9BRF1; A6NI89; B2RE97
UniProt Related Accession #
Q16658
GenBank Nucleotide #
NM_003088.4
NCBI Official Full Name
fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
UniProt Protein Name
Fascin
UniProt Synonym Protein Names
55 kDa actin-bundling protein; Singed-like protein; p55
Protein Family
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
NCBI Protein Information
fascin

NCBI Summary for FSCN1

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
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UniProt Comments for FSCN1

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
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Research Articles on FSCN1

1. Fascin1 is related with clinicopathologic parameters of gastric cancer and overexpressed both in gastric cell lines and gastric tumor tissue

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FSCN1 antibody

 Products by Pathway  Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-FSCN1 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >25 publications with FSCN1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >10 publications with FSCN1 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >7 publications with FSCN1 and Ovarian Neoplasms
 Urinary Bladder Neoplasms Antibodies  >6 publications with FSCN1 and Urinary Bladder Neoplasms
 Lung Neoplasms Antibodies  >6 publications with FSCN1 and Lung Neoplasms
 Liver Neoplasms Antibodies  >5 publications with FSCN1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with FSCN1 and Carcinoma, Hepatocellular
 Lung Diseases Antibodies  >5 publications with FSCN1 and Lung Diseases
 Pancreatic Neoplasms Antibodies  >4 publications with FSCN1 and Pancreatic Neoplasms
 Liver Diseases Antibodies  >4 publications with FSCN1 and Liver Diseases

Organs/Tissues associated with anti-FSCN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >13 publications with FSCN1 and Lung
 Lymph Node Antibodies  >13 publications with FSCN1 and Lymph Node
 Blood Antibodies  >10 publications with FSCN1 and Blood
 Stomach Antibodies  >9 publications with FSCN1 and Stomach
 Bladder Antibodies  >8 publications with FSCN1 and Bladder
 Brain Antibodies  >7 publications with FSCN1 and Brain
 Liver Antibodies  >5 publications with FSCN1 and Liver
 Bone Antibodies  >5 publications with FSCN1 and Bone
 Vascular Antibodies  >4 publications with FSCN1 and Vascular
 Muscle Antibodies  >4 publications with FSCN1 and Muscle
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