• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PHOX2B Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752185

Rabbit IgG Monoclonal antibody for Q99453 detection. Tested positive for WB IHC IF/IF in Human Mouse More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHOX2B, Monoclonal Antibody
Full Product Name
Anti-PHOX2B Monoclonal Antibody
Product Gene Name
anti-PHOX2B antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
31,621 Da
3D Structure
ModBase 3D Structure for Q99453
Immunogen
A synthesized peptide derived from human PHOX2B Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PHOX2B antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PHOX2B antibody
WB: 1:500-1:2000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-PHOX2B antibody

Figure 1. Western blot analysis of PHOX2B using anti-PHOX2B antibody (M02221).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHOX2B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PHOX2B.
Rabbit Anti-PHOX2B Monoclonal Antibody – Cat# MBS1752185

Other Notes

Small volumes of anti-PHOX2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHOX2B purchase

MBS1752185 is an antibody targeting PHOX2B. Also known as Anti-PHOX2B Monoclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PHOX2B. It may not necessarily be applicable to this product.

NCBI GI #
12707580
NCBI GeneID
8929
NCBI Accession #
NP_003915.2
UniProt Primary Accession #
Q99453
UniProt Secondary Accession #
Q6PJD9
UniProt Related Accession #
Q99453
GenBank Nucleotide #
NM_003924.3
NCBI Official Full Name
paired mesoderm homeobox protein 2B
NCBI Official Synonym Full Names
paired like homeobox 2B
NCBI Official Symbol
PHOX2B
NCBI Official Synonym Symbols
CCHS; PMX2B; NBLST2; NBPhox
UniProt Protein Name
Paired mesoderm homeobox protein 2B
UniProt Synonym Protein Names
Neuroblastoma Phox; NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B
Protein Family
Paired mesoderm homeobox protein
UniProt Gene Name
PHOX2B
UniProt Synonym Gene Names
PMX2B; NBPhox
NCBI Protein Information
paired mesoderm homeobox protein 2B

NCBI Summary for PHOX2B

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome. [provided by RefSeq, Jul 2016]
Show more

UniProt Comments for PHOX2B

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
Show more

Research Articles on PHOX2B

1. in vitro transactivation of the RET promoter by different Hirschsprung disease-associated PHOX2B polyA variants has resulted significantly lower compared to the effect of PHOX2B wild type protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PHOX2B antibody

 Reference Product  PubMed Publications
 RET antibody  >32 publications with PHOX2B and RET
 DBH antibody  >31 publications with PHOX2B and DBH
 ASCL1 antibody  >25 publications with PHOX2B and ASCL1
 ALK antibody  >12 publications with PHOX2B and ALK
 EDN3 antibody  >4 publications with PHOX2B and EDN3
 RGS4 antibody  >1 publications with PHOX2B and RGS4

Pathways associated with anti-PHOX2B antibody

 Products by Pathway  Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram

Diseases associated with anti-PHOX2B antibody

 Disease Name  Pubmed Publications
 Congenital central hypoventilation syndrome Antibodies  >142 publications with PHOX2B and Congenital central hypoventilation syndrome
 Neuroblastoma Antibodies  >70 publications with PHOX2B and Neuroblastoma
 Hirschsprung Disease Antibodies  >56 publications with PHOX2B and Hirschsprung Disease
 Congenital Abnormalities Antibodies  >55 publications with PHOX2B and Congenital Abnormalities
 Central Nervous System Diseases Antibodies  >33 publications with PHOX2B and Central Nervous System Diseases
 Chromosome Aberrations Antibodies  >14 publications with PHOX2B and Chromosome Aberrations
 Embryo Loss Antibodies  >9 publications with PHOX2B and Embryo Loss
 Crohn Disease Antibodies  >5 publications with PHOX2B and Crohn Disease
 Weight Gain Antibodies  >4 publications with PHOX2B and Weight Gain
 Neurobehavioral Manifestations Antibodies  >3 publications with PHOX2B and Neurobehavioral Manifestations

Organs/Tissues associated with anti-PHOX2B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >118 publications with PHOX2B and Brain
 Embryonic Tissue Antibodies  >43 publications with PHOX2B and Embryonic Tissue
 Lung Antibodies  >11 publications with PHOX2B and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000