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Hemopexin (HPX) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752186

Rabbit IgG Monoclonal antibody for P02790 detection. Tested positive for WB IHC IF/IF IP in Human More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hemopexin (HPX), Monoclonal Antibody
Full Product Name
Anti-Hemopexin Monoclonal Antibody
Product Gene Name
anti-HPX antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
51,676 Da
3D Structure
ModBase 3D Structure for P02790
Immunogen
A synthesized peptide derived from human Hemopexin Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HPX antibody
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-HPX antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-HPX antibody

Figure 1. Western blot analysis of HPX using anti-HPX antibody (M02237).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPX antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HPX.
Rabbit Anti-HPX Monoclonal Antibody – Cat# MBS1752186

Other Notes

Small volumes of anti-HPX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HPX purchase

MBS1752186 is an antibody targeting HPX. Also known as Anti-Hemopexin Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HPX. It may not necessarily be applicable to this product.

NCBI GI #
11321561
NCBI GeneID
3263
NCBI Accession #
NP_000604.1
UniProt Primary Accession #
P02790
UniProt Secondary Accession #
B2R957
UniProt Related Accession #
P02790
GenBank Nucleotide #
NM_000613.3
NCBI Official Full Name
hemopexin
NCBI Official Synonym Full Names
hemopexin
NCBI Official Symbol
HPX
NCBI Official Synonym Symbols
HX
UniProt Protein Name
Hemopexin
UniProt Synonym Protein Names
Beta-1B-glycoprotein
Protein Family
Hemopexin
UniProt Gene Name
HPX
NCBI Protein Information
hemopexin

NCBI Summary for HPX

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

UniProt Comments for HPX

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.MiscellaneousThe isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear ().
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Research Articles on HPX

1. Data suggest that apo-hemopexin isolated from plasma exchibits low endogenous levels of tyrosine nitration in the peptide YYCFQGNQFLR in the heme-binding site of human hemopexin, which was similarly nitrated in rabbit and rat hemopexins; heme binding by hemopexin declined as tyrosine nitration proceeded in vitro.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HPX antibody

 Reference Product  PubMed Publications
 ALB antibody  >3 publications with HPX and ALB
 APOA2 antibody  >1 publications with HPX and APOA2
 AHSG antibody  >1 publications with HPX and AHSG

Pathways associated with anti-HPX antibody

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Scavenging Of Heme From Plasma Pathway antibodies  Scavenging Of Heme From Plasma Pathway Diagram

Diseases associated with anti-HPX antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >27 publications with HPX and Neoplasms
 Inflammation Antibodies  >17 publications with HPX and Inflammation
 Liver Diseases Antibodies  >14 publications with HPX and Liver Diseases
 Anemia Antibodies  >13 publications with HPX and Anemia
 Liver Neoplasms Antibodies  >8 publications with HPX and Liver Neoplasms
 Brain Diseases Antibodies  >8 publications with HPX and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >7 publications with HPX and Carcinoma, Hepatocellular
 Immune System Diseases Antibodies  >7 publications with HPX and Immune System Diseases
 Death Antibodies  >6 publications with HPX and Death
 Neoplasms, Experimental Antibodies  >5 publications with HPX and Neoplasms, Experimental

Organs/Tissues associated with anti-HPX antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >94 publications with HPX and Blood
 Liver Antibodies  >57 publications with HPX and Liver
 Brain Antibodies  >38 publications with HPX and Brain
 Intestine Antibodies  >12 publications with HPX and Intestine
 Bone Antibodies  >9 publications with HPX and Bone
 Heart Antibodies  >8 publications with HPX and Heart
 Spleen Antibodies  >6 publications with HPX and Spleen
 Connective Tissue Antibodies  >5 publications with HPX and Connective Tissue
 Eye Antibodies  >3 publications with HPX and Eye
 Embryonic Tissue Antibodies  >2 publications with HPX and Embryonic Tissue
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