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IMP3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752197

Rabbit IgG Monoclonal antibody for O00425 detection. Tested positive for WB IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IMP3, Monoclonal Antibody
Full Product Name
Anti-IMP3 Monoclonal Antibody
Product Gene Name
anti-IMP3 antibody
Product Synonym Gene Name
IGF2BP3
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
21,630 Da
3D Structure
ModBase 3D Structure for O00425
Immunogen
A synthesized peptide derived from human IMP3 RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IMP3 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-IMP3 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-IMP3 antibody

Figure 1. Western blot analysis of IGF2BP3 using anti-IGF2BP3 antibody (M02362-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2BP3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGF2BP3.
Rabbit Anti-IMP3 Monoclonal Antibody – Cat# MBS1752197

Other Notes

Small volumes of anti-IMP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IMP3 purchase

MBS1752197 is an antibody targeting IMP3. Also known as Anti-IMP3 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IMP3. It may not necessarily be applicable to this product.

NCBI GI #
30795212
NCBI GeneID
10643
NCBI Accession #
NP_006538.2
UniProt Primary Accession #
O00425
UniProt Secondary Accession #
Q63HM0; Q6MZZ2; Q86VB1; A0A4Z5
UniProt Related Accession #
O00425
GenBank Nucleotide #
NM_006547.3
NCBI Official Full Name
insulin-like growth factor 2 mRNA-binding protein 3
NCBI Official Synonym Full Names
insulin like growth factor 2 mRNA binding protein 3
NCBI Official Symbol
IGF2BP3
NCBI Official Synonym Symbols
KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
UniProt Protein Name
Insulin-like growth factor 2 mRNA-binding protein 3
UniProt Synonym Protein Names
IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3
Protein Family
U3 small nucleolar ribonucleoprotein protein
UniProt Gene Name
IGF2BP3
UniProt Synonym Gene Names
IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
NCBI Protein Information
insulin-like growth factor 2 mRNA-binding protein 3

NCBI Summary for IMP3

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]
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UniProt Comments for IMP3

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
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Research Articles on IMP3

1. Data show that insulin-like growth factor-II mRNA-binding protein 2 and 3 (IMP2/3)-miR-200a-progesterone receptor axis represents a double-negative feedback loop and serves as a new potential therapeutic target for the treatment of Triple-negative breast cancer (TNBC).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IMP3 antibody

 Products by Pathway  Pathway Diagram
 Binding Of RNA By Insulin-like Growth Factor-2 MRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) Pathway antibodies  Binding Of RNA By Insulin-like Growth Factor-2 MRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram

Diseases associated with anti-IMP3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with IMP3 and Neoplasms
 Carcinoma Antibodies  >10 publications with IMP3 and Carcinoma
 Pancreatic Neoplasms Antibodies  >2 publications with IMP3 and Pancreatic Neoplasms
 Liver Diseases Antibodies  >2 publications with IMP3 and Liver Diseases
 Ovarian Neoplasms Antibodies  >2 publications with IMP3 and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with IMP3 and Neoplasms, Experimental
 Recurrence Antibodies  >1 publications with IMP3 and Recurrence
 Liver Neoplasms Antibodies  >1 publications with IMP3 and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >1 publications with IMP3 and Neoplasm Metastasis
 Abnormalities, Multiple Antibodies  >1 publications with IMP3 and Abnormalities, Multiple

Organs/Tissues associated with anti-IMP3 antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >2 publications with IMP3 and Pancreas
 Blood Antibodies  >2 publications with IMP3 and Blood
 Liver Antibodies  >2 publications with IMP3 and Liver
 Brain Antibodies  >2 publications with IMP3 and Brain
 Placenta Antibodies  >2 publications with IMP3 and Placenta
 Embryonic Tissue Antibodies  >2 publications with IMP3 and Embryonic Tissue
 Lung Antibodies  >1 publications with IMP3 and Lung
 Uterus Antibodies  >1 publications with IMP3 and Uterus
 Lymph Antibodies  >1 publications with IMP3 and Lymph
 Bone Marrow Antibodies  >1 publications with IMP3 and Bone Marrow
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