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MBD3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752217

Rabbit IgG Monoclonal antibody for O95983 detection. Tested positive for WB IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MBD3, Monoclonal Antibody
Full Product Name
Anti-MBD3 Monoclonal Antibody
Product Gene Name
anti-MBD3 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
29,014 Da
3D Structure
ModBase 3D Structure for O95983
Immunogen
A synthesized peptide derived from human MBD3 Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MBD3 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MBD3 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Western Blot (WB) of anti-MBD3 antibody

Figure 1. Western blot analysis of MBD3 using anti-MBD3 antibody (M02571-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MBD3.
Rabbit Anti-MBD3 Monoclonal Antibody – Cat# MBS1752217

Other Notes

Small volumes of anti-MBD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MBD3 purchase

MBS1752217 is an antibody targeting MBD3. Also known as Anti-MBD3 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MBD3. It may not necessarily be applicable to this product.

NCBI GI #
527498244
NCBI GeneID
53615
NCBI Accession #
NP_001268382.1
UniProt Primary Accession #
O95983
UniProt Secondary Accession #
Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
UniProt Related Accession #
O95983
GenBank Nucleotide #
NM_001281453.2
NCBI Official Full Name
methyl-CpG-binding domain protein 3 isoform 1
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 3
NCBI Official Symbol
MBD3
UniProt Protein Name
Methyl-CpG-binding domain protein 3
UniProt Synonym Protein Names
Methyl-CpG-binding protein MBD3
Protein Family
Methyl-CpG-binding domain-containing protein
UniProt Gene Name
MBD3
NCBI Protein Information
methyl-CpG-binding domain protein 3

NCBI Summary for MBD3

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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UniProt Comments for MBD3

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
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Research Articles on MBD3

1. Study have demonstrated that MBD3/ NuRD inhibits formation of liver induced cancer stem cells (iCSCs). In addition, MBD3 suppression induces c-JUN, resulting in the induction of pluripotent genes in iCSCs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MBD3 antibody

 Reference Product  PubMed Publications
 MBD2 antibody  >69 publications with MBD3 and MBD2
 HDAC1 antibody  >19 publications with MBD3 and HDAC1
 HDAC2 antibody  >9 publications with MBD3 and HDAC2
 CHD4 antibody  >8 publications with MBD3 and CHD4
 MTA1 antibody  >7 publications with MBD3 and MTA1
 MTA2 antibody  >7 publications with MBD3 and MTA2
 RBBP7 antibody  >3 publications with MBD3 and RBBP7
 GATAD2A antibody  >2 publications with MBD3 and GATAD2A
 RBBP4 antibody  >2 publications with MBD3 and RBBP4
 GATAD2B antibody  >2 publications with MBD3 and GATAD2B

Pathways associated with anti-MBD3 antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HDACs Deacetylate Histones Pathway antibodies  HDACs Deacetylate Histones Pathway Diagram
 RNA Polymerase I Promoter Clearance Pathway antibodies  RNA Polymerase I Promoter Clearance Pathway Diagram
 RNA Polymerase I Transcription Pathway antibodies  RNA Polymerase I Transcription Pathway Diagram
 RNA Polymerase I Transcription Initiation Pathway antibodies  RNA Polymerase I Transcription Initiation Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with anti-MBD3 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >8 publications with MBD3 and Disease Models, Animal
 Nervous System Diseases Antibodies  >6 publications with MBD3 and Nervous System Diseases
 Lung Neoplasms Antibodies  >5 publications with MBD3 and Lung Neoplasms
 Carcinoma Antibodies  >5 publications with MBD3 and Carcinoma
 Liver Neoplasms Antibodies  >2 publications with MBD3 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with MBD3 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >2 publications with MBD3 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with MBD3 and Neoplasm Metastasis
 Inflammation Antibodies  >2 publications with MBD3 and Inflammation
 Autistic Disorder Antibodies  >2 publications with MBD3 and Autistic Disorder

Organs/Tissues associated with anti-MBD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with MBD3 and Brain
 Blood Antibodies  >12 publications with MBD3 and Blood
 Skin Antibodies  >11 publications with MBD3 and Skin
 Lung Antibodies  >7 publications with MBD3 and Lung
 Liver Antibodies  >7 publications with MBD3 and Liver
 Embryonic Tissue Antibodies  >5 publications with MBD3 and Embryonic Tissue
 Nerve Antibodies  >5 publications with MBD3 and Nerve
 Testis Antibodies  >4 publications with MBD3 and Testis
 Eye Antibodies  >2 publications with MBD3 and Eye
 Connective Tissue Antibodies  >2 publications with MBD3 and Connective Tissue
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