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PAK3 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752254

Rabbit IgG Monoclonal antibody for O75914 detection. Tested positive for WB IHC IF IP in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAK3, Monoclonal Antibody
Full Product Name
Anti-PAK3 Monoclonal Antibody
Product Gene Name
anti-PAK3 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
60,693 Da
3D Structure
ModBase 3D Structure for O75914
Immunogen
A synthesized peptide derived from human PAK3 PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PAK3 antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PAK3 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP:1:40

Western Blot (WB) of anti-PAK3 antibody

Figure 1. Western blot analysis of PAK3 using anti-PAK3 antibody (M03124).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAK3.
Rabbit Anti-PAK3 Monoclonal Antibody – Cat# MBS1752254

Other Notes

Small volumes of anti-PAK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAK3 purchase

MBS1752254 is an antibody targeting PAK3. Also known as Anti-PAK3 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAK3. It may not necessarily be applicable to this product.

NCBI GI #
189491751
NCBI GeneID
5063
NCBI Accession #
NP_001121638.1
UniProt Primary Accession #
O75914
UniProt Secondary Accession #
Q5JWX1; Q5JWX2; Q7Z2D6; Q7Z2E4; Q7Z3Z8; Q8WWK5; Q8WX23; A8K389; B1GX77; B1GX78; B1GX79
UniProt Related Accession #
O75914
GenBank Nucleotide #
NM_001128166.2
NCBI Official Full Name
serine/threonine-protein kinase PAK 3 isoform a
NCBI Official Synonym Full Names
p21 (RAC1) activated kinase 3
NCBI Official Symbol
PAK3
NCBI Official Synonym Symbols
ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
UniProt Protein Name
Serine/threonine-protein kinase PAK 3
UniProt Synonym Protein Names
Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
PAK3
UniProt Synonym Gene Names
OPHN3; PAK-3
NCBI Protein Information
serine/threonine-protein kinase PAK 3

NCBI Summary for PAK3

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]
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UniProt Comments for PAK3

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
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Research Articles on PAK3

1. The miR-193b-3p/PAK3 axis might be a potential novel therapeutic target for OC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAK3 antibody

 Reference Product  PubMed Publications
 ARHGEF6 antibody  >10 publications with PAK3 and ARHGEF6
 GIT1 antibody  >3 publications with PAK3 and GIT1
 GIT2 antibody  >2 publications with PAK3 and GIT2
 SYN1 antibody  >1 publications with PAK3 and SYN1
 RAF1 antibody  >1 publications with PAK3 and RAF1
 NCK1 antibody  >1 publications with PAK3 and NCK1
 tax antibody  >1 publications with PAK3 and tax

Pathways associated with anti-PAK3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram

Diseases associated with anti-PAK3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with PAK3 and Neoplasms
 Congenital Abnormalities Antibodies  >6 publications with PAK3 and Congenital Abnormalities
 Carcinoma Antibodies  >3 publications with PAK3 and Carcinoma
 Learning Disorders Antibodies  >3 publications with PAK3 and Learning Disorders
 Memory Disorders Antibodies  >3 publications with PAK3 and Memory Disorders
 Kidney Diseases Antibodies  >2 publications with PAK3 and Kidney Diseases
 Disease Models, Animal Antibodies  >2 publications with PAK3 and Disease Models, Animal
 Immune System Diseases Antibodies  >2 publications with PAK3 and Immune System Diseases
 Lung Diseases Antibodies  >1 publications with PAK3 and Lung Diseases
 Drug Toxicity Antibodies  >1 publications with PAK3 and Drug Toxicity

Organs/Tissues associated with anti-PAK3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >41 publications with PAK3 and Brain
 Eye Antibodies  >4 publications with PAK3 and Eye
 Testis Antibodies  >4 publications with PAK3 and Testis
 Kidney Antibodies  >3 publications with PAK3 and Kidney
 Lung Antibodies  >3 publications with PAK3 and Lung
 Liver Antibodies  >2 publications with PAK3 and Liver
 Spleen Antibodies  >1 publications with PAK3 and Spleen
 Adrenal Gland Antibodies  >1 publications with PAK3 and Adrenal Gland
 Pituitary Gland Antibodies  >1 publications with PAK3 and Pituitary Gland
 Embryonic Tissue Antibodies  >1 publications with PAK3 and Embryonic Tissue
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