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ABHD5 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752257

Rabbit IgG Monoclonal antibody for Q8WTS1 detection. Tested positive for WB IHC FC in Human More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ABHD5, Monoclonal Antibody
Full Product Name
Anti-ABHD5 Monoclonal Antibody
Product Gene Name
anti-ABHD5 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
39,096 Da
3D Structure
ModBase 3D Structure for Q8WTS1
Immunogen
A synthesized peptide derived from human ABHD5 Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ABHD5 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ABHD5 antibody
WB: 1:500-1:2000
IHC: 1:100-1:500
FC/FACS: 1:100

Western Blot (WB) of anti-ABHD5 antibody

Figure 1. Western blot analysis of ABHD5 using anti-ABHD5 antibody (M03209).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABHD5.
Rabbit Anti-ABHD5 Monoclonal Antibody – Cat# MBS1752257

Other Notes

Small volumes of anti-ABHD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ABHD5 purchase

MBS1752257 is an antibody targeting ABHD5. Also known as Anti-ABHD5 Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ABHD5. It may not necessarily be applicable to this product.

NCBI GI #
31542303
NCBI GeneID
51099
NCBI Accession #
NP_057090.2
UniProt Primary Accession #
Q8WTS1
UniProt Secondary Accession #
Q9Y369; B2R9K0
UniProt Related Accession #
Q8WTS1
GenBank Nucleotide #
NM_016006.5
NCBI Official Full Name
1-acylglycerol-3-phosphate O-acyltransferase ABHD5 isoform a
NCBI Official Synonym Full Names
abhydrolase domain containing 5
NCBI Official Symbol
ABHD5
NCBI Official Synonym Symbols
CGI58; IECN2; NCIE2
UniProt Protein Name
1-acylglycerol-3-phosphate O-acyltransferase ABHD5
UniProt Synonym Protein Names
Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58
Protein Family
1-acylglycerol-3-phosphate O-acyltransferase
UniProt Gene Name
ABHD5
UniProt Synonym Gene Names
NCIE2
NCBI Protein Information
1-acylglycerol-3-phosphate O-acyltransferase ABHD5

NCBI Summary for ABHD5

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]
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UniProt Comments for ABHD5

Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion ().
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Research Articles on ABHD5

1. our findings indicate that inhibition of both DGAT1 and ABHD5 using siRNA leads to reduction in prostate cancer cell growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ABHD5 antibody

 Reference Product  PubMed Publications
 PNPLA2 antibody  >27 publications with ABHD5 and PNPLA2
 PLIN1 antibody  >4 publications with ABHD5 and PLIN1

Pathways associated with anti-ABHD5 antibody

 Products by Pathway  Pathway Diagram
 CDP-diacylglycerol Biosynthesis Pathway antibodies  CDP-diacylglycerol Biosynthesis Pathway Diagram
 Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies  Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Triacylglycerol Biosynthesis Pathway antibodies  Triacylglycerol Biosynthesis Pathway Diagram

Diseases associated with anti-ABHD5 antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >23 publications with ABHD5 and Fatty Liver
 Liver Diseases Antibodies  >21 publications with ABHD5 and Liver Diseases
 Muscular Diseases Antibodies  >14 publications with ABHD5 and Muscular Diseases
 Triglyceride storage disease with impaired long-chain fatty acid oxidation Antibodies  >9 publications with ABHD5 and Triglyceride storage disease with impaired long-chain fatty acid oxidation
 Hepatomegaly Antibodies  >7 publications with ABHD5 and Hepatomegaly
 Inflammation Antibodies  >5 publications with ABHD5 and Inflammation
 Cardiovascular Diseases Antibodies  >5 publications with ABHD5 and Cardiovascular Diseases
 Heart Diseases Antibodies  >5 publications with ABHD5 and Heart Diseases
 Necrosis Antibodies  >4 publications with ABHD5 and Necrosis
 Carcinoma, Hepatocellular Antibodies  >3 publications with ABHD5 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-ABHD5 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >34 publications with ABHD5 and Liver
 Connective Tissue Antibodies  >21 publications with ABHD5 and Connective Tissue
 Muscle Antibodies  >20 publications with ABHD5 and Muscle
 Skin Antibodies  >20 publications with ABHD5 and Skin
 Blood Antibodies  >15 publications with ABHD5 and Blood
 Heart Antibodies  >7 publications with ABHD5 and Heart
 Intestine Antibodies  >3 publications with ABHD5 and Intestine
 Bone Antibodies  >3 publications with ABHD5 and Bone
 Kidney Antibodies  >2 publications with ABHD5 and Kidney
 Bone Marrow Antibodies  >2 publications with ABHD5 and Bone Marrow
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