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TRIM25 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752258

Rabbit IgG Monoclonal antibody for Q14258 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TRIM25, Monoclonal Antibody
Full Product Name
Anti-TRIM25 Monoclonal Antibody
Product Gene Name
anti-TRIM25 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
70,973 Da
3D Structure
ModBase 3D Structure for Q14258
Immunogen
A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRIM25 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TRIM25 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:100-1:500
IP: 1:50
FC/FACS: 1:60

Western Blot (WB) of anti-TRIM25 antibody

Figure 1. Western blot analysis of TRIM25 using anti-TRIM25 antibody (M03232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRIM25.
Rabbit Anti-TRIM25 Monoclonal Antibody – Cat# MBS1752258

Other Notes

Small volumes of anti-TRIM25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM25 purchase

MBS1752258 is an antibody targeting TRIM25. Also known as Anti-TRIM25 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM25. It may not necessarily be applicable to this product.

NCBI GI #
68160937
NCBI GeneID
7706
NCBI Accession #
NP_005073.2
UniProt Primary Accession #
Q14258
UniProt Related Accession #
Q14258
GenBank Nucleotide #
NM_005082.5
NCBI Official Full Name
E3 ubiquitin/ISG15 ligase TRIM25
NCBI Official Synonym Full Names
tripartite motif containing 25
NCBI Official Symbol
TRIM25
NCBI Official Synonym Symbols
EFP; Z147; RNF147; ZNF147
UniProt Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Synonym Protein Names
Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase (EC:2.3.2.271 PublicationManual assertion based on experiment iniRef.9"TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity."Gack M.U., Shin Y.C., Joo C.H., Urano T., Liang C., Sun L., Takeuchi O., Akira S., Chen Z., Inoue S., Jung J.U.Nature 446:916-920(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS AN UBIQUITIN LIGASE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, INTERACTION WITH DDX58.
Protein Family
E3 ubiquitin/ISG15 ligase
UniProt Gene Name
TRIM25
UniProt Synonym Gene Names
EFP; RNF147; ZNF147
NCBI Protein Information
E3 ubiquitin/ISG15 ligase TRIM25

NCBI Summary for TRIM25

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRIM25

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
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Research Articles on TRIM25

1. The RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain, which we postulate to be a novel RNA-binding domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIM25 antibody

 Reference Product  PubMed Publications
 SFN antibody  >3 publications with TRIM25 and SFN

Pathways associated with anti-TRIM25 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 NF-kB Activation Through FADD/RIP-1 Pathway Mediated By Caspase-8 And -10 antibodies  NF-kB Activation Through FADD/RIP-1 Pathway Mediated By Caspase-8 And -10 Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram

Diseases associated with anti-TRIM25 antibody

 Disease Name  Pubmed Publications
 Endometrial Neoplasms Antibodies  >3 publications with TRIM25 and Endometrial Neoplasms
 Brain Diseases Antibodies  >2 publications with TRIM25 and Brain Diseases
 Liver Diseases Antibodies  >1 publications with TRIM25 and Liver Diseases
 Heart Diseases Antibodies  >1 publications with TRIM25 and Heart Diseases
 Prostatic Diseases Antibodies  >1 publications with TRIM25 and Prostatic Diseases
 Drug Toxicity Antibodies  >1 publications with TRIM25 and Drug Toxicity
 Hyperplasia Antibodies  >1 publications with TRIM25 and Hyperplasia

Organs/Tissues associated with anti-TRIM25 antibody

 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >8 publications with TRIM25 and Uterus
 Ovary Antibodies  >4 publications with TRIM25 and Ovary
 Mammary Gland Antibodies  >3 publications with TRIM25 and Mammary Gland
 Brain Antibodies  >2 publications with TRIM25 and Brain
 Vascular Antibodies  >2 publications with TRIM25 and Vascular
 Lymph Node Antibodies  >2 publications with TRIM25 and Lymph Node
 Kidney Antibodies  >2 publications with TRIM25 and Kidney
 Spleen Antibodies  >2 publications with TRIM25 and Spleen
 Lymph Antibodies  >2 publications with TRIM25 and Lymph
 Embryonic Tissue Antibodies  >2 publications with TRIM25 and Embryonic Tissue
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