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TRAP Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752262

Rabbit IgG Monoclonal antibody for P13686 detection. Tested positive for WB IHC IF/IF IP in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TRAP, Monoclonal Antibody
Full Product Name
Anti-TRAP Monoclonal Antibody
Product Gene Name
anti-TRAP antibody
Product Synonym Gene Name
ACP5
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
36,599 Da
3D Structure
ModBase 3D Structure for P13686
Immunogen
A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRAP antibody
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TRAP antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-TRAP antibody

Figure 1. Western blot analysis of ACP5 using anti-ACP5 antibody (M03277-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP5 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACP5.
Rabbit Anti-TRAP Monoclonal Antibody – Cat# MBS1752262

Other Notes

Small volumes of anti-TRAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRAP purchase

MBS1752262 is an antibody targeting TRAP. Also known as Anti-TRAP Monoclonal Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRAP. It may not necessarily be applicable to this product.

NCBI GI #
161377451
NCBI GeneID
54
NCBI Accession #
NP_001104504.1
UniProt Primary Accession #
P13686
UniProt Secondary Accession #
Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2
UniProt Related Accession #
P13686
GenBank Nucleotide #
NM_001111034.2
NCBI Official Full Name
tartrate-resistant acid phosphatase type 5
NCBI Official Synonym Full Names
acid phosphatase 5, tartrate resistant
NCBI Official Symbol
ACP5
NCBI Official Synonym Symbols
HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
UniProt Protein Name
Tartrate-resistant acid phosphatase type 5
UniProt Synonym Protein Names
Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
Protein Family
Protein
UniProt Gene Name
ACP5
UniProt Synonym Gene Names
TR-AP; TrATPase
NCBI Protein Information
tartrate-resistant acid phosphatase type 5

NCBI Summary for TRAP

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

UniProt Comments for TRAP

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

Research Articles on TRAP

1. high expression of ACP5 correlates with tumor progression and may serve as a potential prognostic biomarker in lung AD.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRAP antibody

 Reference Product  PubMed Publications
 TNFSF11 antibody  >106 publications with TRAP and TNFSF11
 CTSK antibody  >77 publications with TRAP and CTSK
 TNFRSF11B antibody  >31 publications with TRAP and TNFRSF11B
 SPP1 antibody  >12 publications with TRAP and SPP1
 BGLAP antibody  >8 publications with TRAP and BGLAP
 ENPP1 antibody  >1 publications with TRAP and ENPP1

Pathways associated with anti-TRAP antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 Osteoclast Signaling Pathway antibodies  Osteoclast Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 RANKL/RANK Signaling Pathway antibodies  RANKL/RANK Signaling Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram

Diseases associated with anti-TRAP antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with TRAP and Inflammation
 Spondyloenchondrodysplasia Antibodies  >6 publications with TRAP and Spondyloenchondrodysplasia
 Liver Diseases Antibodies  >4 publications with TRAP and Liver Diseases
 Necrosis Antibodies  >4 publications with TRAP and Necrosis
 Obesity Antibodies  >3 publications with TRAP and Obesity
 Liver Neoplasms Antibodies  >2 publications with TRAP and Liver Neoplasms
 Lung Diseases Antibodies  >2 publications with TRAP and Lung Diseases
 Disease Models, Animal Antibodies  >2 publications with TRAP and Disease Models, Animal
 Lung Neoplasms Antibodies  >2 publications with TRAP and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with TRAP and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-TRAP antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >52 publications with TRAP and Bone
 Blood Antibodies  >9 publications with TRAP and Blood
 Liver Antibodies  >8 publications with TRAP and Liver
 Connective Tissue Antibodies  >8 publications with TRAP and Connective Tissue
 Lung Antibodies  >3 publications with TRAP and Lung
 Uterus Antibodies  >2 publications with TRAP and Uterus
 Kidney Antibodies  >2 publications with TRAP and Kidney
 Brain Antibodies  >2 publications with TRAP and Brain
 Eye Antibodies  >1 publications with TRAP and Eye
 Parathyroid Antibodies  >1 publications with TRAP and Parathyroid
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