Rabbit Anti-NRCAM Monoclonal Antibody – Cat# MBS1752296

Figure 1. Western blot analysis of NRCAM using anti-NRCAM antibody (MBS1752296).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRCAM antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NRCAM.

NRCAM Antibody – Rabbit Monoclonal

Name: NRCAM Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752296
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752296

Anti-NRCAM Monoclonal Antibody MBS1752296. Tested in IP, WB applications. This antibody reacts with Human. More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NRCAM, Monoclonal Antibody

Full Product Name

Anti-NRCAM Monoclonal Antibody

Product Gene Name

anti-NRCAM antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

ACOH-14

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA

Purity/Purification

Affinity-Chromatography

Preparation and Storage

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

3D Structure

ModBase 3D Structure for Q92823

Immunogen

A synthesized peptide derived from human NRCAM Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-NRCAM antibody

Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-NRCAM antibody

Dilute the sample so that the expected range of concentrations fall within the detection range of this kit.
If the expected range of concentration is unknown, a pilot test should be conducted to decide the optimal dilution ratio for your samples.
Some PubMed article(s) citing the expression level of this target are as follows: Internal QC testing used:
WB: 1:1000~1:5000
IP: 1:20

Other Notes

Small volumes of anti-NRCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NRCAM purchase

MBS1752296 is an antibody targeting NRCAM. Also known as Anti-NRCAM Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NRCAM. It may not necessarily be applicable to this product.

NCBI GI #

81158226

NCBI GeneID

4897

NCBI Accession #

NP_001032209.1

UniProt Primary Accession #

Q92823

UniProt Secondary Accession #

O15051; O15179; Q14BM2; Q9UHI3; Q9UHI4; A4D0S3; E9PDA4

UniProt Related Accession #

Q92823

GenBank Nucleotide #

NM_001037132.3

NCBI Official Full Name

neuronal cell adhesion molecule isoform A

NCBI Official Synonym Full Names

neuronal cell adhesion molecule

NCBI Official Symbol

NRCAM

UniProt Protein Name

Neuronal cell adhesion molecule

UniProt Synonym Protein Names

Neuronal surface protein Bravo

Protein Family

Neuronal cell adhesion molecule

UniProt Gene Name

NRCAM

UniProt Synonym Gene Names

KIAA0343; hBravo1 PublicationManual assertion based on opinion iniRef.1; Ng-CAM-related

NCBI Protein Information

neuronal cell adhesion molecule

NCBI Summary for NRCAM

Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for NRCAM

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.

Research Articles on NRCAM

1. Increased gene expression of NrCAM may be used as a potential diagnostic marker for papillary thyroid cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NRCAM antibody

Reference Product	PubMed Publications
NFASC antibody	>20 publications with NRCAM and NFASC
CNTN2 antibody	>16 publications with NRCAM and CNTN2
ANK3 antibody	>5 publications with NRCAM and ANK3
GLDN antibody	>3 publications with NRCAM and GLDN
NRP2 antibody	>2 publications with NRCAM and NRP2
ANK2 antibody	>1 publications with NRCAM and ANK2

Pathways associated with anti-NRCAM antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Interaction Between L1 And Ankyrins Pathway antibodies	Interaction Between L1 And Ankyrins Pathway Diagram
L1CAM Interactions Pathway antibodies	L1CAM Interactions Pathway Diagram
Neurofascin Interactions Pathway antibodies	Neurofascin Interactions Pathway Diagram
NrCAM Interactions Pathway antibodies	NrCAM Interactions Pathway Diagram

Diseases associated with anti-NRCAM antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>14 publications with NRCAM and Brain Diseases
Autistic Disorder Antibodies	>6 publications with NRCAM and Autistic Disorder
Breast Neoplasms Antibodies	>6 publications with NRCAM and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>4 publications with NRCAM and Cell Transformation, Neoplastic
Inflammation Antibodies	>4 publications with NRCAM and Inflammation
Substance-Related Disorders Antibodies	>3 publications with NRCAM and Substance-Related Disorders
Neoplasms, Experimental Antibodies	>3 publications with NRCAM and Neoplasms, Experimental
Hyperplasia Antibodies	>2 publications with NRCAM and Hyperplasia
Learning Disorders Antibodies	>2 publications with NRCAM and Learning Disorders
Colorectal Neoplasms Antibodies	>2 publications with NRCAM and Colorectal Neoplasms

Organs/Tissues associated with anti-NRCAM antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>71 publications with NRCAM and Brain
Nerve Antibodies	>57 publications with NRCAM and Nerve
Eye Antibodies	>17 publications with NRCAM and Eye
Embryonic Tissue Antibodies	>14 publications with NRCAM and Embryonic Tissue
Blood Antibodies	>13 publications with NRCAM and Blood
Muscle Antibodies	>6 publications with NRCAM and Muscle
Vascular Antibodies	>6 publications with NRCAM and Vascular
Liver Antibodies	>4 publications with NRCAM and Liver
Kidney Antibodies	>4 publications with NRCAM and Kidney
Heart Antibodies	>3 publications with NRCAM and Heart