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ERAB Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752301

Rabbit IgG Monoclonal antibody for Q99714 detection. Tested positive for WB IHC IF/IF FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERAB, Monoclonal Antibody
Full Product Name
Anti-ERAB Monoclonal Antibody
Product Gene Name
anti-ERAB antibody
Product Synonym Gene Name
HSD17B10
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
25,984 Da
3D Structure
ModBase 3D Structure for Q99714
Immunogen
A synthesized peptide derived from human ERAB Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ERAB antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ERAB antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-ERAB antibody

Figure 1. Western blot analysis of HSD17B10 using anti-HSD17B10 antibody (M03844).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B10 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSD17B10.
Rabbit Anti-ERAB Monoclonal Antibody – Cat# MBS1752301

Other Notes

Small volumes of anti-ERAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ERAB purchase

MBS1752301 is an antibody targeting ERAB. Also known as Anti-ERAB Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ERAB. It may not necessarily be applicable to this product.

NCBI GI #
83715985
NCBI GeneID
3028
NCBI Accession #
NP_001032900.1
UniProt Primary Accession #
Q99714
UniProt Secondary Accession #
Q5H927; Q6IBS9; Q8TCV9; Q96HD5
UniProt Related Accession #
Q99714
GenBank Nucleotide #
NM_001037811.2
NCBI Official Full Name
3-hydroxyacyl-CoA dehydrogenase type-2 isoform 2
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 10
NCBI Official Symbol
HSD17B10
NCBI Official Synonym Symbols
ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; HSD10MD; 17b-HSD10; DUPXp11.22
UniProt Protein Name
3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 10 (EC:1.1.1.512 PublicationsManual assertion based on experiment iniRef.10"Expanded substrate screenings of human and Drosophila type 10 17beta-hydroxysteroid dehydrogenases (HSDs) reveal multiple specificities in bile acid and steroid hormone metabolism: characterization of multifunctional 3alpha/7alpha/7beta/17beta/20beta/21-HSD."Shafqat N., Marschall H.U., Filling C., Nordling E., Wu X.Q., Bjork L., Thyberg J., Martensson E., Salim S., Jornvall H., Oppermann U.Biochem. J. 376:49-60(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
Protein Family
Erabutoxin
UniProt Gene Name
HSD17B10
UniProt Synonym Gene Names
ERAB; HADH2; MRPP2; SCHAD; SDR5C1; XH98G2; MHBD1 PublicationManual assertion based on opinion iniRef.24; Mitochondrial RNase P protein 2
NCBI Protein Information
3-hydroxyacyl-CoA dehydrogenase type-2

NCBI Summary for ERAB

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
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UniProt Comments for ERAB

Mitochondrial dehydrogenase that catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone (PubMed:9553139, PubMed:23042678, PubMed:12917011, PubMed:18996107, PubMed:25925575, PubMed:28888424). Catalyzes the third step in the beta-oxidation of fatty acids (PubMed:9553139, PubMed:12917011, PubMed:18996107, PubMed:25925575, PubMed:28888424). Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids (PubMed:12917011). Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids (PubMed:12917011). By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) (PubMed:9338779). Essential for structural and functional integrity of mitochondria (PubMed:20077426).
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Research Articles on ERAB

1. in addition to being an essential component of the RNase P reaction, MRPP1/2 serves as a processing platform for several down-stream tRNA maturation steps in human mitochondria.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ERAB antibody

 Reference Product  PubMed Publications
 APP antibody  >3 publications with ERAB and APP

Pathways associated with anti-ERAB antibody

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Branched-chain Amino Acid Catabolism Pathway antibodies  Branched-chain Amino Acid Catabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Valine, Leucine And Isoleucine Degradation Pathway antibodies  Valine, Leucine And Isoleucine Degradation Pathway Diagram
 Valine, Leucine And Isoleucine Degradation Pathway antibodies  Valine, Leucine And Isoleucine Degradation Pathway Diagram
 Fatty Acid Beta-oxidation I Pathway antibodies  Fatty Acid Beta-oxidation I Pathway Diagram

Diseases associated with anti-ERAB antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >41 publications with ERAB and Nervous System Diseases
 Neurobehavioral Manifestations Antibodies  >9 publications with ERAB and Neurobehavioral Manifestations
 Cardiovascular Diseases Antibodies  >4 publications with ERAB and Cardiovascular Diseases
 Neoplasms Antibodies  >3 publications with ERAB and Neoplasms
 Fatty Liver Antibodies  >3 publications with ERAB and Fatty Liver
 Diabetes Mellitus Antibodies  >3 publications with ERAB and Diabetes Mellitus
 Prostatic Diseases Antibodies  >3 publications with ERAB and Prostatic Diseases
 Heart Diseases Antibodies  >2 publications with ERAB and Heart Diseases
 Liver Diseases Antibodies  >2 publications with ERAB and Liver Diseases
 Prostatic Neoplasms Antibodies  >2 publications with ERAB and Prostatic Neoplasms

Organs/Tissues associated with anti-ERAB antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >29 publications with ERAB and Brain
 Blood Antibodies  >6 publications with ERAB and Blood
 Muscle Antibodies  >6 publications with ERAB and Muscle
 Liver Antibodies  >6 publications with ERAB and Liver
 Prostate Antibodies  >4 publications with ERAB and Prostate
 Testis Antibodies  >3 publications with ERAB and Testis
 Nerve Antibodies  >3 publications with ERAB and Nerve
 Vascular Antibodies  >3 publications with ERAB and Vascular
 Heart Antibodies  >3 publications with ERAB and Heart
 Embryonic Tissue Antibodies  >2 publications with ERAB and Embryonic Tissue
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