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KLKB1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752343

Rabbit IgG Monoclonal antibody for P03952 detection. Tested positive for WB IHC IP in Human Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLKB1, Monoclonal Antibody
Full Product Name
Anti-KLKB1 Monoclonal Antibody
Product Gene Name
anti-KLKB1 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
71,370 Da
3D Structure
ModBase 3D Structure for P03952
Immunogen
A synthesized peptide derived from human KLKB1 The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KLKB1 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-KLKB1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50

Western Blot (WB) of anti-KLKB1 antibody

Figure 1. Western blot analysis of KLKB1 using anti-KLKB1 antibody (M04995).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLKB1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KLKB1.
Rabbit Anti-KLKB1 Monoclonal Antibody – Cat# MBS1752343

Other Notes

Small volumes of anti-KLKB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLKB1 purchase

MBS1752343 is an antibody targeting KLKB1. Also known as Anti-KLKB1 Monoclonal Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KLKB1. It may not necessarily be applicable to this product.

NCBI GI #
78191798
NCBI GeneID
3818
NCBI Accession #
NP_000883.2
UniProt Primary Accession #
P03952
UniProt Secondary Accession #
Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9
UniProt Related Accession #
P03952
GenBank Nucleotide #
NM_000892.5
NCBI Official Full Name
plasma kallikrein isoform 1 preproprotein
NCBI Official Synonym Full Names
kallikrein B1
NCBI Official Symbol
KLKB1
NCBI Official Synonym Symbols
PKK; PPK; KLK3; PKKD
UniProt Protein Name
Plasma kallikrein
UniProt Synonym Protein Names
Fletcher factor; Kininogenin; Plasma prekallikrein; PKK
Protein Family
Plasma kallikrein
UniProt Gene Name
KLKB1
UniProt Synonym Gene Names
KLK3; PKK
NCBI Protein Information
plasma kallikrein

NCBI Summary for KLKB1

This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
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UniProt Comments for KLKB1

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Research Articles on KLKB1

1. We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLKB1 antibody

 Reference Product  PubMed Publications
 F12 antibody  >2 publications with KLKB1 and F12
 KNG1 antibody  >1 publications with KLKB1 and KNG1

Pathways associated with anti-KLKB1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram

Diseases associated with anti-KLKB1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >5 publications with KLKB1 and Cardiovascular Diseases
 Hypertension Antibodies  >4 publications with KLKB1 and Hypertension
 Neoplasms Antibodies  >4 publications with KLKB1 and Neoplasms
 Prekallikrein Deficiency Antibodies  >3 publications with KLKB1 and Prekallikrein Deficiency
 Lung Neoplasms Antibodies  >2 publications with KLKB1 and Lung Neoplasms
 Mental Disorders Antibodies  >1 publications with KLKB1 and Mental Disorders
 Death Antibodies  >1 publications with KLKB1 and Death
 Prostatic Diseases Antibodies  >1 publications with KLKB1 and Prostatic Diseases
 Inflammation Antibodies  >1 publications with KLKB1 and Inflammation
 Kidney Diseases Antibodies  >1 publications with KLKB1 and Kidney Diseases

Organs/Tissues associated with anti-KLKB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2 publications with KLKB1 and Heart
 Kidney Antibodies  >2 publications with KLKB1 and Kidney
 Connective Tissue Antibodies  >1 publications with KLKB1 and Connective Tissue
 Mammary Gland Antibodies  >1 publications with KLKB1 and Mammary Gland
 Brain Antibodies  >1 publications with KLKB1 and Brain
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