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ZIC1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752361

Rabbit IgG Monoclonal antibody for Q15915 detection. Tested positive for WB IF/IF FC in Human Mouse Rat More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Zic1, Monoclonal Antibody
Full Product Name
Anti-Zic1 Monoclonal Antibody
Product Gene Name
anti-Zic1 antibody
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-Chromatography
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
Molecular Weight
48,309 Da
3D Structure
ModBase 3D Structure for Q15915
Immunogen
A synthesized peptide derived from human Zic1 Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Zic1 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-Zic1 antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:120

Western Blot (WB) of anti-Zic1 antibody

Figure 1. Western blot analysis of ZIC1 using anti-ZIC1 antibody (M05537).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZIC1.
Rabbit Anti-ZIC1 Monoclonal Antibody – Cat# MBS1752361

Other Notes

Small volumes of anti-Zic1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Zic1 purchase

MBS1752361 is an antibody targeting Zic1. Also known as Anti-Zic1 Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Zic1. It may not necessarily be applicable to this product.

NCBI GI #
22547182
NCBI GeneID
7545
NCBI Accession #
NP_003403.2
UniProt Primary Accession #
Q15915
UniProt Secondary Accession #
Q2M3N1
UniProt Related Accession #
Q15915
GenBank Nucleotide #
NM_003412.4
NCBI Official Full Name
zinc finger protein ZIC 1
NCBI Official Synonym Full Names
Zic family member 1
NCBI Official Symbol
ZIC1
NCBI Official Synonym Symbols
ZIC; CRS6; ZNF201
UniProt Protein Name
Zinc finger protein ZIC 1
UniProt Synonym Protein Names
Zinc finger protein 201; Zinc finger protein of the cerebellum 1
Protein Family
Zinc finger protein
UniProt Gene Name
ZIC1
UniProt Synonym Gene Names
ZIC; ZNF201
NCBI Protein Information
zinc finger protein ZIC 1

NCBI Summary for Zic1

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for Zic1

Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' ().
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Research Articles on Zic1

1. Study demonstrate that ZIC1 plays a tumor suppressive role in breast cancer, by targeting surviving, significantly downregulating its expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Zic1 antibody

 Reference Product  PubMed Publications
 GLI3 antibody  >4 publications with Zic1 and GLI3
 GLI1 antibody  >4 publications with Zic1 and GLI1
 GLI2 antibody  >3 publications with Zic1 and GLI2

Diseases associated with anti-Zic1 antibody

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >15 publications with Zic1 and Congenital Abnormalities
 Nervous System Malformations Antibodies  >14 publications with Zic1 and Nervous System Malformations
 Neoplasms Antibodies  >10 publications with Zic1 and Neoplasms
 Neuroectodermal Tumors Antibodies  >3 publications with Zic1 and Neuroectodermal Tumors
 Craniofacial Abnormalities Antibodies  >3 publications with Zic1 and Craniofacial Abnormalities
 Carcinoma Antibodies  >3 publications with Zic1 and Carcinoma
 Immune System Diseases Antibodies  >2 publications with Zic1 and Immune System Diseases
 Blood Coagulation Disorders Antibodies  >1 publications with Zic1 and Blood Coagulation Disorders
 Uterine Neoplasms Antibodies  >1 publications with Zic1 and Uterine Neoplasms
 Pain Antibodies  >1 publications with Zic1 and Pain

Organs/Tissues associated with anti-Zic1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >55 publications with Zic1 and Brain
 Embryonic Tissue Antibodies  >22 publications with Zic1 and Embryonic Tissue
 Muscle Antibodies  >7 publications with Zic1 and Muscle
 Ear Antibodies  >4 publications with Zic1 and Ear
 Eye Antibodies  >2 publications with Zic1 and Eye
 Testis Antibodies  >1 publications with Zic1 and Testis
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