Rabbit Anti-NAPSA Monoclonal Antibody – Cat# MBS1752366

Figure 1. Western blot analysis of NAPSA using anti-NAPSA antibody (M05685-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAPSA antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NAPSA.

Napsin A (NAPSA) Antibody – Rabbit Monoclonal

Name: NAPSA Antibody; Monoclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752366
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752366

Rabbit IgG Monoclonal antibody for O96009 detection. Tested positive for WB IHC IP in Human More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Napsin A (NAPSA), Monoclonal Antibody

Full Product Name

Anti-Napsin A Monoclonal Antibody

Product Gene Name

anti-NAPSA antibody

Clonality

Monoclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

Affinity-Chromatography

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.

Molecular Weight

45,387 Da

3D Structure

ModBase 3D Structure for O96009

Immunogen

A synthesized peptide derived from human NAPSIN A May be involved in processing of pneumocyte surfactant precursors.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-NAPSA antibody

Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-NAPSA antibody

WB: 1:1000-1:5000
IHC: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-NAPSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAPSA purchase

MBS1752366 is an antibody targeting NAPSA. Also known as Anti-Napsin A Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NAPSA. It may not necessarily be applicable to this product.

NCBI GI #

4758754

NCBI GeneID

9476

NCBI Accession #

NP_004842.1

UniProt Primary Accession #

O96009

UniProt Secondary Accession #

Q8WWD9

UniProt Related Accession #

O96009

GenBank Nucleotide #

NM_004851.3

NCBI Official Full Name

napsin-A preproprotein

NCBI Official Synonym Full Names

napsin A aspartic peptidase

NCBI Official Symbol

NAPSA

NCBI Official Synonym Symbols

KAP; Kdap; NAP1; NAPA; SNAPA

UniProt Protein Name

Napsin-A

UniProt Synonym Protein Names

Aspartyl protease 4; ASP4; Asp 4; Napsin-1; TA01/TA02

Protein Family

Napsin

UniProt Gene Name

NAPSA

UniProt Synonym Gene Names

NAP1; NAPA; ASP4; Asp 4

NCBI Protein Information

napsin-A

NCBI Summary for NAPSA

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]

UniProt Comments for NAPSA

May be involved in processing of pneumocyte surfactant precursors.

Research Articles on NAPSA

1. These data indicated that napsin A expression may inhibit TGFbetalinduced EMT and was negatively associated with EMTmediated erlotinib resistance, suggesting that napsin A expression may improve the sensitivity of lung cancer cells to EGFRTKI through the inhibition of EMT.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NAPSA antibody

Products by Pathway	Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Surfactant Metabolism Pathway antibodies	Surfactant Metabolism Pathway Diagram

Diseases associated with anti-NAPSA antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>9 publications with NAPSA and Kidney Diseases
Liver Neoplasms Antibodies	>4 publications with NAPSA and Liver Neoplasms
Fibrosis Antibodies	>3 publications with NAPSA and Fibrosis
Neoplasms, Experimental Antibodies	>3 publications with NAPSA and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>2 publications with NAPSA and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>2 publications with NAPSA and Liver Diseases
Cardiovascular Diseases Antibodies	>1 publications with NAPSA and Cardiovascular Diseases
Hyperplasia Antibodies	>1 publications with NAPSA and Hyperplasia
Disease Models, Animal Antibodies	>1 publications with NAPSA and Disease Models, Animal
Skin Neoplasms Antibodies	>1 publications with NAPSA and Skin Neoplasms

Organs/Tissues associated with anti-NAPSA antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>80 publications with NAPSA and Lung
Kidney Antibodies	>18 publications with NAPSA and Kidney
Uterus Antibodies	>3 publications with NAPSA and Uterus
Prostate Antibodies	>3 publications with NAPSA and Prostate
Spleen Antibodies	>2 publications with NAPSA and Spleen
Trachea Antibodies	>1 publications with NAPSA and Trachea