ORAI1 Antibody – Rabbit Polyclonal

Description: Rabbit IgG polyclonal antibody for Orai1 detection. Tested with WB, FCM, Direct ELISA in Human; Mouse; Rat.

Background: ORAI1(ORAI calcium release-activated calcium modulator 1), also known as  CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective ion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency(SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

1. Prakriya, M., Feske, S., Gwack, Y., Srikanth, S., Rao, A., Hogan, P. G. Orai1 is an essential pore subunit of the CRAC channel. Nature 443: 230-233, 2006.

Small volumes of anti-Orai1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1752456 is an antibody targeting Orai1. Reactive in Human, Mouse, Rat. Host: Rabbit.