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PTBP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752515

Description: Rabbit IgG polyclonal antibody for PTBP2 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human; Mouse; Rat.Background: Polypyrimidine tract binding protein 2, also known as PTBP2, is a protein which in humans is encoded by the… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PTBP2, Polyclonal Antibody
Full Product Name
Anti-PTBP2 Antibody
Product Gene Name
Product Synonym Names
Polypyrimidine tract-binding protein 2; polypyrimidine tract binding protein 2; Polypyrimidine tract-binding protein 2; Neural polypyrimidine tract-binding protein; Neurally-enriched homolog of PTB; PTB-like protein; PTBP2; NPTB; PTB; PTBLP
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
AF176085 mRNA
3D Structure
ModBase 3D Structure for Q9UKA9
Immunogen
E Coli-derived human PTBP2 recombinant protein (Position: M1-A504).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P), IHC(F) and ICC.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PTBP2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-PTBP2 antibody
WB: 0.1-0.25ug/ml
IHC-P: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml
ICC: 0.5-1ug/ml
FC/FACS: 1-3mug/1x106
Direct ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-PTBP2 antibody

1. Makeyev, E. V., Zhang, J., Carrasco, M. A., Maniatis, T. The microRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing. Molec. Cell 27: 435-448, 2007. 2. Rahman, L., Bliskovski, V., Kaye, F. J., Zajac-Kaye, M. Evolutionary conservation of a 2-kb intronic sequence flanking a tissue-specific alternative exon in the PTBP2 gene. Genomics 83: 76-84, 2004.

Other Notes

Small volumes of anti-PTBP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTBP2 purchase

MBS1752515 is an antibody targeting PTBP2. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTBP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
polypyrimidine tract-binding protein 2 isoform 1
NCBI Official Synonym Full Names
polypyrimidine tract binding protein 2
NCBI Official Symbol
PTBP2
NCBI Official Synonym Symbols
nPTB; PTBLP; brPTB
UniProt Protein Name
Polypyrimidine tract-binding protein 2
UniProt Synonym Protein Names
Neural polypyrimidine tract-binding protein; Neurally-enriched homolog of PTB; PTB-like protein
UniProt Gene Name
PTBP2
UniProt Synonym Gene Names
NPTB; PTB; PTBLP
NCBI Protein Information
polypyrimidine tract-binding protein 2

NCBI Summary for PTBP2

The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
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UniProt Comments for PTBP2

PTBP2: RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre- mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cell soma; growth cone; spliceosomal complex

Molecular Function: mRNA binding; RNA binding

Biological Process: cerebellum development; mRNA splice site selection; negative regulation of RNA splicing; regulation of neural precursor cell proliferation; spinal cord development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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