Rabbit Anti-GJA4 Polyclonal Antibody – Cat# MBS1752522

Connexin 37/GJA4 (GJA4) Antibody – Rabbit Polyclonal

Name: GJA4 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752522
Price: 390 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752522

Description: Rabbit IgG polyclonal antibody for Connexin 37/GJA4 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human; Mouse; Rat.Background: Gap junction alpha-4 protein (GJA4), also called CX37 is a protein that in humans is encoded by… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Connexin 37/GJA4, Polyclonal Antibody

Full Product Name

Anti-Connexin 37/GJA4 Antibody

Product Gene Name

anti-GJA4 antibody

Product Synonym Names

Gap junction alpha-4 protein; gap junction protein alpha 4; Gap junction alpha-4 protein; Connexin-37; Cx37; GJA4

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Specificity

No cross reactivity with other proteins.

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

121012

3D Structure

ModBase 3D Structure for P35212

Immunogen

E Coli-derived human Connexin 37/GJA4 recombinant protein (Position: M1-Q271).

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Relevant Detection Systems

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P), IHC(F) and ICC.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500mug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-GJA4 antibody

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Application Notes for anti-GJA4 antibody

WB: 0.25-0.5ug/ml
IHC-P: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml
ICC: 0.5-1ug/ml
FC/FACS: 1-3mug/1x106
Direct ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-GJA4 antibody

1. Tsai, M.-Y., Lan, K.-C., Huang, K.-E., Huang, F.-J., Kung, F.-T., Chang, S.-Y. Significance of mRNA levels of connexin37, connexin43, and connexin45 in luteinized granulosa cells of controlled hyperstimulated follicles. Fertil. Steril. 80: 1437-1443, 2003. 2. Wong, C. W., Christen, T., Roth, I., Chadjichristos, C. E., Deroutte, J.-P., Foglia, B. F., Chanson, M., Goodenough, D. A., Kwak, B. R. Connexin37 protects against atherosclerosis by regulating monocyte adhesion. Nature Med. 12: 950-954, 2006. 3. Yamada, Y., Izawa, H., Ichihara, S., Takatsu, F., Ishihara, H., Hirayama, H., Sone, T., Tanaka, M., Yokota, M. Prediction of the risk of myocardial infarction from polymorphisms in candidate genes. New Eng. J. Med. 347: 1916-1923, 2002.

Other Notes

Small volumes of anti-GJA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GJA4 purchase

MBS1752522 is an antibody targeting GJA4. Also known as Anti-Connexin 37/GJA4 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GJA4. It may not necessarily be applicable to this product.

NCBI GI #

62865885

NCBI GeneID

2701

NCBI Accession #

NP_002051.2

UniProt Primary Accession #

P35212

UniProt Related Accession #

P35212

GenBank Nucleotide #

NP_002051.2

NCBI Official Full Name

gap junction alpha-4 protein

NCBI Official Synonym Full Names

gap junction protein alpha 4

NCBI Official Symbol

GJA4

NCBI Official Synonym Symbols

CX37

UniProt Protein Name

Gap junction alpha-4 protein

UniProt Synonym Protein Names

Connexin-37

Protein Family

Gap junction alpha-4 protein

UniProt Gene Name

GJA4

UniProt Synonym Gene Names

Cx37

UniProt Entry Name

CXA4_HUMAN

NCBI Protein Information

gap junction alpha-4 protein

NCBI Summary for GJA4

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]

UniProt Comments for GJA4

GJA4: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: connexon complex; integral to plasma membrane; gap junction

Molecular Function: protein binding

Biological Process: blood vessel development; intercellular junction assembly; cell-cell signaling; transport; calcium ion transport; response to pain

Research Articles on GJA4

1. Data show that fluid shear stress activates NOTCH signaling, which upregulates GJA4 (commonly, Cx37) and downstream cell cycle inhibitor CDKN1B (p27).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GJA4 antibody

Products by Pathway	Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Gap Junction Assembly Pathway antibodies	Gap Junction Assembly Pathway Diagram
Gap Junction Trafficking Pathway antibodies	Gap Junction Trafficking Pathway Diagram
Gap Junction Trafficking And Regulation Pathway antibodies	Gap Junction Trafficking And Regulation Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Ovarian Infertility Genes Pathway antibodies	Ovarian Infertility Genes Pathway Diagram

Diseases associated with anti-GJA4 antibody

Disease Name	Pubmed Publications
Atherosclerosis Antibodies	>6 publications with GJA4 and Atherosclerosis
Myocardial Infarction Antibodies	>6 publications with GJA4 and Myocardial Infarction
Nervous System Diseases Antibodies	>4 publications with GJA4 and Nervous System Diseases
Hypertension Antibodies	>4 publications with GJA4 and Hypertension
Insulin Resistance Antibodies	>2 publications with GJA4 and Insulin Resistance
Neoplasms Antibodies	>1 publications with GJA4 and Neoplasms
Inflammation Antibodies	>1 publications with GJA4 and Inflammation

Organs/Tissues associated with anti-GJA4 antibody

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>4 publications with GJA4 and Ovary
Heart Antibodies	>3 publications with GJA4 and Heart
Vascular Antibodies	>3 publications with GJA4 and Vascular
Skin Antibodies	>2 publications with GJA4 and Skin
Bone Antibodies	>1 publications with GJA4 and Bone
Nerve Antibodies	>1 publications with GJA4 and Nerve
Brain Antibodies	>1 publications with GJA4 and Brain