Rabbit Anti-SMARCAD1 Polyclonal Antibody – Cat# MBS1752526

SMARCAD1 Antibody – Rabbit Polyclonal

Name: SMARCAD1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1752526
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752526

Description: Rabbit IgG polyclonal antibody for SMARCAD1 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 is a protein… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SMARCAD1, Polyclonal Antibody

Full Product Name

Anti-SMARCAD1 Antibody

Product Gene Name

anti-SMARCAD1 antibody

Product Synonym Names

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1; SMARCAD1; KIAA1122

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized

Purity/Purification

Immunogen Affinity Purified

Specificity

No cross reactivity with other proteins.

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

136000

3D Structure

ModBase 3D Structure for Q9H4L7

Immunogen

E Coli-derived human SMARCAD1 recombinant protein (Position: D827-T1002).

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Relevant Detection Systems

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500mug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SMARCAD1 antibody

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)

Application Notes for anti-SMARCAD1 antibody

WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-SMARCAD1 antibody

1. Nousbeck, J., Burger, B., Fuchs-Telem, D., Pavlovsky, M., Fenig, S., Sarig, O., Itin, P., Sprecher, E. A mutation in a skin-specific isoform of SMARCAD1 causes autosomal-dominant adermatoglyphia. Am. J. Hum. Genet. 89: 302-307, 2011. 2. Nousbeck, J., Sarig, O., Magal, L., Warschauer, E., Burger, B., Itin, P., Sprecher, E. Mutations in SMARCAD1 cause autosomal dominant adermatoglyphia and perturb the expression of epidermal differentiation-associated genes. Brit. J. Derm. 171: 1521-1524, 2014. 3. Okazaki, N., Ikeda, S., Ohara, R., Shimada, K., Yanagawa, T., Nagase, T., Ohara, O., Koga, H. The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSS. J. Molec. Biol. 382: 257-265, 2008.

Other Notes

Small volumes of anti-SMARCAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMARCAD1 purchase

MBS1752526 is an antibody targeting SMARCAD1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCAD1. It may not necessarily be applicable to this product.

NCBI GI #

363808412

NCBI GeneID

56916

NCBI Accession #

NP_001241878.1

UniProt Primary Accession #

Q9H4L7

UniProt Related Accession #

Q9H4L7

GenBank Nucleotide #

NP_001241878.1

NCBI Official Full Name

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 isoform c

NCBI Official Synonym Full Names

SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1

NCBI Official Symbol

SMARCAD1

NCBI Official Synonym Symbols

HRZ; ETL1; HEL1; ADERM; BASNS

UniProt Protein Name

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

UniProt Synonym Protein Names

ATP-dependent helicase 1

Protein Family

UniProt Gene Name

SMARCAD1

UniProt Synonym Gene Names

KIAA1122; hHEL1

UniProt Entry Name

SMRCD_HUMAN

NCBI Protein Information

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

NCBI Summary for SMARCAD1

This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for SMARCAD1

SMARCAD1: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.12; Helicase; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q22.3

Cellular Component: nucleoplasm; heterochromatin; nuclear matrix; nuclear replication fork

Molecular Function: protein binding; nucleic acid binding; DNA binding; helicase activity; ATP binding

Biological Process: chromatin remodeling; regulation of DNA recombination; positive regulation of transcription, DNA-dependent; ATP-dependent chromatin remodeling; DNA double-strand break processing; nucleotide metabolic process; chromatin modification; chromosome separation; protein homooligomerization

Disease: Adermatoglyphia

Research Articles on SMARCAD1

1. Whole-genome sequencing on DNA from affected individuals with Huriez syndrome from three families revealed three not previously reported SNPs in SMARCAD1 gene. Western blot experiments confirmed the reduced expression of Smarcad1 protein in keratinocytes and fibroblasts from patients compared with healthy controls. Study concluded that haploinsufficiency for the skin-specific SMARCAD1 isoform accounts for Huriez syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMARCAD1 antibody

Reference Product	PubMed Publications
SMARCA5 antibody	>1 publications with SMARCAD1 and SMARCA5
TRIM28 antibody	>1 publications with SMARCAD1 and TRIM28

Diseases associated with anti-SMARCAD1 antibody

Disease Name	Pubmed Publications
Liver Neoplasms Antibodies	>2 publications with SMARCAD1 and Liver Neoplasms
Liver Diseases Antibodies	>2 publications with SMARCAD1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>2 publications with SMARCAD1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SMARCAD1 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2 publications with SMARCAD1 and Liver
Skin Antibodies	>1 publications with SMARCAD1 and Skin
Embryonic Tissue Antibodies	>1 publications with SMARCAD1 and Embryonic Tissue
Eye Antibodies	>1 publications with SMARCAD1 and Eye
Brain Antibodies	>1 publications with SMARCAD1 and Brain