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TRAF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752917

Description: Rabbit IgG polyclonal antibody for TRAF6 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse; Rat.Background: TNF receptor-associated factor 6, also called E3 ubiquitin-protein ligase TRAF6 or RNF85, is a TRAF human protein. The protein… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRAF6, Polyclonal Antibody
Full Product Name
Anti-TRAF6 Antibody
Product Gene Name
anti-Traf6 antibody
Product Synonym Names
TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; RING-type E3 ubiquitin transferase TRAF6; Traf6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P70196
Immunogen
E Coli-derived mouse TRAF6 recombinant protein (Position: Q14-D512).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Traf6 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-Traf6 antibody
WB: 0.25-0.5ug/ml (Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Flow Cytometry (FC/FACS) of anti-Traf6 antibody

Rabbit Anti-TRAF6 Polyclonal Antibody – Cat# MBS1752917

Immunohistochemistry (IHC) of anti-Traf6 antibody

Rabbit Anti-TRAF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1752917

Immunohistochemistry (IHC) of anti-Traf6 antibody

Rabbit Anti-TRAF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1752917

Western Blot (WB) of anti-Traf6 antibody

Rabbit Anti-TRAF6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1752917

Product References and Citations for anti-Traf6 antibody

1. Akiyama, T., Maeda, S., Yamane, S., Ogino, K., Kasai, M., Kajiura, F., Matsumoto, M., Inoue, J. Dependence of self-tolerance on TRAF6-directed development of thymic stroma. Science 308: 248-251, 2005.
2. King, C. G., Kobayashi, T., Cejas, P. J., Kim, T., Yoon, K., Kim, G. K., Chiffoleau, E., Hickman, S. P., Walsh, P. T., Turka, L. A., Choi, Y. TRAF6 is a T cell-intrinsic negative regulator required for the maintenance of immune homeostasis. Nature Med. 12: 1088-1092, 2006.

Other Notes

Small volumes of anti-Traf6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Traf6 purchase

MBS1752917 is an antibody targeting Traf6. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Traf6. It may not necessarily be applicable to this product.

NCBI GI #
38348246
NCBI GeneID
22034
NCBI Accession #
NP_033450.2
UniProt Primary Accession #
P70196
UniProt Related Accession #
P70196
GenBank Nucleotide #
NP_033450.2
NCBI Official Full Name
TNF receptor-associated factor 6
NCBI Official Synonym Full Names
TNF receptor-associated factor 6
NCBI Official Symbol
Traf6
NCBI Official Synonym Symbols
AI851288; 2310003F17Rik; C630032O20Rik
UniProt Protein Name
TNF receptor-associated factor 6
UniProt Synonym Protein Names
E3 ubiquitin-protein ligase TRAF6
Protein Family
TNF receptor-associated factor
UniProt Gene Name
Traf6
UniProt Entry Name
TRAF6_MOUSE
NCBI Protein Information
TNF receptor-associated factor 6

NCBI Summary for Traf6

This gene encodes a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. The product of this gene is essential for perinatal and postnatal survival. Mice deficient in this protein exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. This protein possesses ubiquitin ligase activity. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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UniProt Comments for Traf6

Function: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.7 PublicationsManual assertion based on experiment in:Ref.5
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Research Articles on Traf6

1. a novel positive feedback regulation of UBL4A in innate immune response combating virus invasion by enhancing the K63-linked ubiquitination of TRAF6.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Traf6 antibody

 Reference Product  PubMed Publications
 Myd88 antibody  >262 publications with Traf6 and Myd88
 Tlr4 antibody  >178 publications with Traf6 and Tlr4
 Irak1 antibody  >119 publications with Traf6 and Irak1
 Cd40 antibody  >108 publications with Traf6 and Cd40
 Ticam1 antibody  >17 publications with Traf6 and Ticam1
 Ikbkg antibody  >16 publications with Traf6 and Ikbkg
 Map3k1 antibody  >7 publications with Traf6 and Map3k1
 Ubc antibody  >5 publications with Traf6 and Ubc
 Map3k7 antibody  >4 publications with Traf6 and Map3k7
 Ecsit antibody  >2 publications with Traf6 and Ecsit

Pathways associated with anti-Traf6 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 EBV LMP1 Signaling Pathway antibodies  EBV LMP1 Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-Traf6 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >639 publications with Traf6 and Necrosis
 Inflammation Antibodies  >285 publications with Traf6 and Inflammation
 Immune System Diseases Antibodies  >95 publications with Traf6 and Immune System Diseases
 Arthritis, Rheumatoid Antibodies  >44 publications with Traf6 and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >38 publications with Traf6 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >36 publications with Traf6 and Nervous System Diseases
 Lung Diseases Antibodies  >25 publications with Traf6 and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >18 publications with Traf6 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >18 publications with Traf6 and Liver Diseases
 Atherosclerosis Antibodies  >15 publications with Traf6 and Atherosclerosis

Organs/Tissues associated with anti-Traf6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >175 publications with Traf6 and Blood
 Muscle Antibodies  >32 publications with Traf6 and Muscle
 Kidney Antibodies  >31 publications with Traf6 and Kidney
 Skin Antibodies  >29 publications with Traf6 and Skin
 Brain Antibodies  >29 publications with Traf6 and Brain
 Liver Antibodies  >22 publications with Traf6 and Liver
 Lymph Node Antibodies  >15 publications with Traf6 and Lymph Node
 Thymus Antibodies  >14 publications with Traf6 and Thymus
 Uterus Antibodies  >4 publications with Traf6 and Uterus
 Trachea Antibodies  >3 publications with Traf6 and Trachea
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