• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KDM6B Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752979

Description: Rabbit IgG polyclonal antibody for KDM6B/JMJD3 detection. Tested with WB, FCM, Direct ELISA in Human;Mouse.Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KDM6B/JMJD3, Polyclonal Antibody
Full Product Name
Anti-KDM6B/JMJD3 Antibody
Product Gene Name
anti-KDM6B antibody
Product Synonym Names
Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B; KDM6B; JMJD3; KIAA0346
Clonality
Polyclonal
Isotype
Rabbit IgG
Species Reactivity
Human, Mouse
Form/Format
Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
611577
3D Structure
ModBase 3D Structure for O15054
Immunogen
E Coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KDM6B antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-KDM6B antibody
WB: 0.25-0.5ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human, Mouse)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

MBS176460 for Western Blot.

Flow Cytometry (FC/FACS) of anti-KDM6B antibody

Anti-KDM6B Polyclonal Antibody – Cat# MBS1752979

Flow Cytometry (FC/FACS) of anti-KDM6B antibody

Anti-KDM6B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1752979

Western Blot (WB) of anti-KDM6B antibody

Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.
Anti-KDM6B Polyclonal Antibody, Western Blot (WB) – Cat# MBS1752979

Product References and Citations for anti-KDM6B antibody

1. Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., Cai, C., Qian, G., Capel, B. The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science 360: 645-648, 2018.
2. Sen, G. L., Webster, D. E., Barragan, D. I., Chang, H. Y., Khavari, P. A. Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev. 22: 1865-1870, 2008.
3. Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller-Ramey, J., Person, R., Wentzensen, I. M., McWalter, K., Keren, B., Heron, B., Nava, C., and 19 others. Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. Am. J. Med. Genet. 179A: 1276-1286, 2019.

Other Notes

Small volumes of anti-KDM6B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM6B purchase

MBS1752979 is an antibody targeting KDM6B. Also known as Anti-KDM6B/JMJD3 Antibody. Reactive in Human, Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KDM6B. It may not necessarily be applicable to this product.

NCBI GI #
119610532
NCBI GeneID
23135
NCBI Accession #
EAW90126
UniProt Primary Accession #
O15054
UniProt Related Accession #
O15054
NCBI Official Full Name
hCG2036579, isoform CRA_a
NCBI Official Synonym Full Names
lysine demethylase 6B
NCBI Official Symbol
KDM6B
NCBI Official Synonym Symbols
JMJD3; NEDCFSA
UniProt Protein Name
Lysine-specific demethylase 6B
UniProt Synonym Protein Names
JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B
Protein Family
Lysine-specific demethylase
UniProt Gene Name
KDM6B
UniProt Synonym Gene Names
JMJD3; KIAA0346
UniProt Entry Name
KDM6B_HUMAN
NCBI Protein Information
lysine-specific demethylase 6B

NCBI Summary for KDM6B

The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]
Show more

UniProt Comments for KDM6B

JMJD3: Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Interacts with TLE1. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and KDM6B. By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Belongs to the UTX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; dioxygenase activity; sequence-specific DNA binding; metal ion binding; beta-catenin binding

Biological Process: endothelial cell differentiation; establishment and/or maintenance of chromatin architecture; mesodermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; cardiac muscle cell differentiation
Show more

Research Articles on KDM6B

1. Knock-out of KDM6s (JMJD3 and/or UTX) in human embryonic stem cells (hESCs) showed that KDM6s (JMJD3 and/or UTX)-deficient human ESCs exit pluripotency and commit to neural progenitor cells (NPC) differentiation normally, but the resulting NPCs fail to transit into neurons and glia due to a lack of accessibility at loci essential for neurogenesis.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000