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HEC1/HEC/NDC80 (NDC80) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753001

Description: Rabbit IgG polyclonal antibody for HEC1/HEC/NDC80 detection. Tested with WB, FCM, Direct ELISA in Human; Mouse; Rat.Background: Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. It is mapped to 18p11.32.… More details

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Product Overview

Online Datasheet in PDF
Product Name
HEC1/HEC/NDC80, Polyclonal Antibody
Full Product Name
Anti-HEC1/HEC/NDC80 Antibody
Product Gene Name
anti-NDC80 antibody
Product Synonym Names
Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; NDC80; HEC, HEC1, KNTC2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
607272
3D Structure
ModBase 3D Structure for O14777
Immunogen
E Coli-derived human HEC1/HEC/NDC80 recombinant protein (Position: N87-E602).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NDC80 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-NDC80 antibody
WB: 0.25-0.5ug/ml (Human, Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-NDC80 antibody

Rabbit Anti-NDC80 Polyclonal Antibody – Cat# MBS1753001

Flow Cytometry (FC/FACS) of anti-NDC80 antibody

Rabbit Anti-NDC80 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753001

Immunofluorescence (IF) of anti-NDC80 antibody

Rabbit Anti-NDC80 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753001

Western Blot (WB) of anti-NDC80 antibody

Rabbit Anti-NDC80 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1753001

Product References and Citations for anti-NDC80 antibody

1. Chen Y, Riley DJ, Chen PL, Lee WH (October 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Molecular and Cellular Biology. 17 (10): 6049-56.
2. Martin-Lluesma S, Stucke VM, Nigg EA (September 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267-70.
3. Chen Y, Sharp ZD, Lee WH (September 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081-7.

Other Notes

Small volumes of anti-NDC80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDC80 purchase

MBS1753001 is an antibody targeting NDC80. Also known as Anti-HEC1/HEC/NDC80 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NDC80. It may not necessarily be applicable to this product.

NCBI GI #
5174457
NCBI GeneID
10403
NCBI Accession #
NP_006092.1
UniProt Primary Accession #
O14777
UniProt Related Accession #
O14777
GenBank Nucleotide #
NP_006092.1
NCBI Official Full Name
kinetochore protein NDC80 homolog
NCBI Official Synonym Full Names
NDC80 kinetochore complex component
NCBI Official Symbol
NDC80
NCBI Official Synonym Symbols
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
UniProt Protein Name
Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names
Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
Protein Family
Kinetochore protein
UniProt Gene Name
NDC80
UniProt Synonym Gene Names
HEC; HEC1; KNTC2; HsHec1
UniProt Entry Name
NDC80_HUMAN
NCBI Protein Information
kinetochore protein NDC80 homolog

NCBI Summary for NDC80

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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UniProt Comments for NDC80

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
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Research Articles on NDC80

1. nuclear division cycle 80, cyclin B2 and topoisomerase 2alpha may serve important roles in adrenocortical tumor development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NDC80 antibody

 Reference Product  PubMed Publications
 NUF2 antibody  >42 publications with NDC80 and NUF2
 MIS12 antibody  >35 publications with NDC80 and MIS12
 SPC25 antibody  >26 publications with NDC80 and SPC25
 ZWINT antibody  >8 publications with NDC80 and ZWINT
 MAD2L1 antibody  >7 publications with NDC80 and MAD2L1
 NEK2 antibody  >4 publications with NDC80 and NEK2
 MAD1L1 antibody  >4 publications with NDC80 and MAD1L1
 AURKA antibody  >1 publications with NDC80 and AURKA

Pathways associated with anti-NDC80 antibody

 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-NDC80 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >20 publications with NDC80 and Neoplasms
 Carcinoma Antibodies  >5 publications with NDC80 and Carcinoma
 Ovarian Neoplasms Antibodies  >4 publications with NDC80 and Ovarian Neoplasms
 Breast Neoplasms Antibodies  >4 publications with NDC80 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with NDC80 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >3 publications with NDC80 and Lung Neoplasms
 Adenocarcinoma Antibodies  >3 publications with NDC80 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >2 publications with NDC80 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >1 publications with NDC80 and Nervous System Diseases
 Precancerous Conditions Antibodies  >1 publications with NDC80 and Precancerous Conditions

Organs/Tissues associated with anti-NDC80 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with NDC80 and Blood
 Lung Antibodies  >3 publications with NDC80 and Lung
 Heart Antibodies  >2 publications with NDC80 and Heart
 Stomach Antibodies  >2 publications with NDC80 and Stomach
 Brain Antibodies  >2 publications with NDC80 and Brain
 Kidney Antibodies  >1 publications with NDC80 and Kidney
 Cervix Antibodies  >1 publications with NDC80 and Cervix
 Ovary Antibodies  >1 publications with NDC80 and Ovary
 Skin Antibodies  >1 publications with NDC80 and Skin
 Testis Antibodies  >1 publications with NDC80 and Testis
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