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Cyclin B2/CCNB2 (CCNB2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753014

Description: Rabbit IgG polyclonal antibody for Cyclin B2/CCNB2 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse; Rat.Background: G2/mitotic-specific cyclin-B2 is a protein that in humans is encoded by the CCNB2 gene. This gene is mapped to… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cyclin B2/CCNB2, Polyclonal Antibody
Full Product Name
Anti-Cyclin B2/CCNB2 Antibody
Product Gene Name
Product Synonym Names
G2/mitotic-specific cyclin-B2; CCNB2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
602755
3D Structure
ModBase 3D Structure for O95067
Immunogen
E Coli-derived human Cyclin B2/CCNB2 recombinant protein (Position: K23-A391).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CCNB2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-CCNB2 antibody
WB: 0.25-0.5ug/ml (Human, Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-CCNB2 antibody

Rabbit Anti-CCNB2 Polyclonal Antibody – Cat# MBS1753014

Immunohistochemistry (IHC) of anti-CCNB2 antibody

Rabbit Anti-CCNB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753014

Immunohistochemistry (IHC) of anti-CCNB2 antibody

Rabbit Anti-CCNB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753014

Western Blot (WB) of anti-CCNB2 antibody

Figure 1. Western blot analysis of CCNB2 using anti-CCNB2 antibody.
Rabbit Anti-CCNB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1753014

Product References and Citations for anti-CCNB2 antibody

1. Brandeis, M., Rosewell, I., Carrington, M., Crompton, T., Jacobs, M. A., Kirk, J., Gannon, J., Hunt, T. Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero. Proc. Nat. Acad. Sci. 95: 4344-4349, 1998.
2. Jackman, M., Firth, M., Pines, J. Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus. EMBO J. 14: 1646-1654, 1995.
3. Liu, J. H., Wei, S., Burnette, P. K., Gamero, A. M., Hutton, M., Djeu, J. Y. Functional association of TGF-beta receptor II with cyclin B. Oncogene 18: 269-275, 1999.

Other Notes

Small volumes of anti-CCNB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCNB2 purchase

MBS1753014 is an antibody targeting CCNB2. Also known as Anti-Cyclin B2/CCNB2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
G2/mitotic-specific cyclin-B2
NCBI Official Synonym Full Names
cyclin B2
NCBI Official Symbol
CCNB2
NCBI Official Synonym Symbols
HsT17299
UniProt Protein Name
G2/mitotic-specific cyclin-B2
UniProt Gene Name
CCNB2
UniProt Entry Name
CCNB2_HUMAN
NCBI Protein Information
G2/mitotic-specific cyclin-B2

NCBI Summary for CCNB2

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
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UniProt Comments for CCNB2

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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