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TRIM25 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753073

Description: Rabbit IgG polyclonal antibody for TRIM25/EFP detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.Background: Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. It is mapped to… More details

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Product Overview

Online Datasheet in PDF
Product Name
TRIM25/EFP, Polyclonal Antibody
Full Product Name
Anti-TRIM25/EFP Antibody
Product Gene Name
anti-TRIM25 antibody
Product Synonym Names
E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; TRIM25; EFP; RNF147; ZNF147
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
600453
3D Structure
ModBase 3D Structure for Q14258
Immunogen
E Coli-derived human TRIM25/EFP recombinant protein (Position: M1-A610).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRIM25 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-TRIM25 antibody
WB: 0.1-0.25ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-TRIM25 antibody

1. Gack, M. U., Shin, Y. C., Joo, C.-H., Urano, T., Liang, C., Sun, L., Takeuchi, O., Akira, S., Chen, Z., Inoue, S., Jung, J. U. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature 446: 916-920, 2007.
2. Inoue, S., Orimo, A., Hosoi, T., Kondo, S., Toyoshima, H., Kondo, T., Ikegami, A., Ouchi, Y., Orimo, H., Muramatsu, M. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Nat. Acad. Sci. 90: 11117-11121, 1993.
3. Manokaran, G., Finol, E., Wang, C., Gunaratne, J., Bahl, J., Ong, E. Z., Tan, H. C., Sessions, O. M., Ward, A. M., Gubler, D. J., Harris, E., Garcia-Blanco, M. A., Ooi, E. E. Dengue subgenomic RNA binds TRIM25 to inhibit interferon expression for epidemiological fitness. Science 350: 217-221, 2015.

Other Notes

Small volumes of anti-TRIM25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM25 purchase

MBS1753073 is an antibody targeting TRIM25. Also known as Anti-TRIM25/EFP Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM25. It may not necessarily be applicable to this product.

NCBI GI #
68160937
NCBI GeneID
7706
NCBI Accession #
NP_005073.2
UniProt Primary Accession #
Q14258
UniProt Related Accession #
Q14258
GenBank Nucleotide #
NP_005073.2
NCBI Official Full Name
E3 ubiquitin/ISG15 ligase TRIM25
NCBI Official Synonym Full Names
tripartite motif containing 25
NCBI Official Symbol
TRIM25
NCBI Official Synonym Symbols
EFP; Z147; RNF147; ZNF147
UniProt Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Synonym Protein Names
Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147
Protein Family
E3 ubiquitin/ISG15 ligase
UniProt Gene Name
TRIM25
UniProt Synonym Gene Names
EFP; RNF147; ZNF147
UniProt Entry Name
TRI25_HUMAN
NCBI Protein Information
E3 ubiquitin/ISG15 ligase TRIM25

NCBI Summary for TRIM25

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRIM25

TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.

Protein type: EC 6.3.2.n3; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription factor; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; negative regulation of interferon type I production; defense response to virus
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Research Articles on TRIM25

1. Riplet, and not TRIM25, is required endogenously for the ubiquitination of RIG-I.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIM25 antibody

 Reference Product  PubMed Publications
 SFN antibody  >3 publications with TRIM25 and SFN

Pathways associated with anti-TRIM25 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 NF-kB Activation Through FADD/RIP-1 Pathway Mediated By Caspase-8 And -10 antibodies  NF-kB Activation Through FADD/RIP-1 Pathway Mediated By Caspase-8 And -10 Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram

Diseases associated with anti-TRIM25 antibody

 Disease Name  Pubmed Publications
 Endometrial Neoplasms Antibodies  >3 publications with TRIM25 and Endometrial Neoplasms
 Brain Diseases Antibodies  >2 publications with TRIM25 and Brain Diseases
 Liver Diseases Antibodies  >1 publications with TRIM25 and Liver Diseases
 Heart Diseases Antibodies  >1 publications with TRIM25 and Heart Diseases
 Prostatic Diseases Antibodies  >1 publications with TRIM25 and Prostatic Diseases
 Drug Toxicity Antibodies  >1 publications with TRIM25 and Drug Toxicity
 Hyperplasia Antibodies  >1 publications with TRIM25 and Hyperplasia

Organs/Tissues associated with anti-TRIM25 antibody

 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >8 publications with TRIM25 and Uterus
 Ovary Antibodies  >4 publications with TRIM25 and Ovary
 Mammary Gland Antibodies  >3 publications with TRIM25 and Mammary Gland
 Brain Antibodies  >2 publications with TRIM25 and Brain
 Vascular Antibodies  >2 publications with TRIM25 and Vascular
 Lymph Node Antibodies  >2 publications with TRIM25 and Lymph Node
 Kidney Antibodies  >2 publications with TRIM25 and Kidney
 Spleen Antibodies  >2 publications with TRIM25 and Spleen
 Lymph Antibodies  >2 publications with TRIM25 and Lymph
 Embryonic Tissue Antibodies  >2 publications with TRIM25 and Embryonic Tissue
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