Rabbit Anti-PTF1A Polyclonal Antibody – Cat# MBS1753093

PTF1A Antibody – Rabbit Polyclonal

Name: PTF1A Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1753093
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753093

Description: Rabbit IgG polyclonal antibody for PTF1A detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.Background: Pancreas transcription factor 1 subunit alpha is a protein that in humans is encoded by the PTF1A gene. It is mapped to… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PTF1A, Polyclonal Antibody

Full Product Name

Anti-PTF1A Antibody

Product Gene Name

anti-PTF1A antibody

Product Synonym Names

Pancreas transcription factor 1 subunit alpha; Class A basic helix-loop-helix protein 29; bHLHa29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48; PTF1A; BHLHA29; PTF1P48

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen affinity purified.

Specificity

No cross reactivity with other proteins.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

607194

3D Structure

ModBase 3D Structure for Q7RTS3

Immunogen

E Coli-derived human PTF1A recombinant protein (Position: M1-Q292).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PTF1A antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Application Notes for anti-PTF1A antibody

WB: 0.25-0.5ug/ml (Human, Mouse, Rat)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-PTF1A antibody

1. Al-Shammari, M., Al-Husain, M., Al-Kharfy, T., Alkuraya, F. S. A novel PTF1A mutation in a patient with severe pancreatic and cerebellar involvement. (Letter) Clin. Genet. 80: 196-198, 2011.
2. Sellick, G. S., Barker, K. T., Stolte-Dijkstra, I., Fleischmann, C., Coleman, R. J., Garrett, C., Gloyn, A. L., Edghill, E. L., Hattersley, A. T., Wellauer, P. K., Goodwin, G., Houlston, R. S. Mutations in PTF1A cause pancreatic and cerebellar agenesis. Nature Genet. 36: 1301-1305, 2004.
3. Weedon, M. N., Cebola, I., Patch, A.-M., Flanagan, S. E., De Franco, E., Caswell, R., Rodriguez-Segui, S. A., Shaw-Smith, C., Cho, C. H.-H., Allen, H. L., Houghton, J. A. L., Roth, C. L., and 9 others. Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis. Nature Genet. 46: 61-64, 2014.

Other Notes

Small volumes of anti-PTF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTF1A purchase

MBS1753093 is an antibody targeting PTF1A. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTF1A. It may not necessarily be applicable to this product.

NCBI GI #

30039710

NCBI GeneID

256297

NCBI Accession #

NP_835455.1

UniProt Primary Accession #

Q7RTS3

UniProt Related Accession #

Q7RTS3

GenBank Nucleotide #

NP_835455.1

NCBI Official Full Name

pancreas transcription factor 1 subunit alpha

NCBI Official Synonym Full Names

pancreas associated transcription factor 1a

NCBI Official Symbol

PTF1A

NCBI Official Synonym Symbols

p48; PACA; PAGEN2; bHLHa29; PTF1-p48

UniProt Protein Name

Pancreas transcription factor 1 subunit alpha

UniProt Synonym Protein Names

Class A basic helix-loop-helix protein 29; bHLHa29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1

Protein Family

Pancreas transcription factor

UniProt Gene Name

PTF1A

UniProt Synonym Gene Names

BHLHA29; PTF1P48; bHLHa29; PTF1-p48

UniProt Entry Name

PTF1A_HUMAN

NCBI Protein Information

pancreas transcription factor 1 subunit alpha

NCBI Summary for PTF1A

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

UniProt Comments for PTF1A

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; sequence-specific DNA binding; chromatin binding

Biological Process: retinoic acid receptor signaling pathway; tissue development; embryonic development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; pancreas development; exocrine pancreas development; neuron fate commitment; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; endocrine pancreas development

Disease: Pancreatic And Cerebellar Agenesis; Pancreatic Agenesis 2

Research Articles on PTF1A

1. non-neural progenitor transcription factor Ptf1a alone is sufficient to directly reprogram mouse and human fibroblasts into self-renewable induced neural stem cells capable of differentiating into functional neurons, astrocytes and oligodendrocytes, and improving cognitive dysfunction of Alzheimer's disease mouse models when transplanted

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTF1A antibody

Reference Product	PubMed Publications
RBPJ antibody	>12 publications with PTF1A and RBPJ
HNF1B antibody	>7 publications with PTF1A and HNF1B
HES1 antibody	>5 publications with PTF1A and HES1
RBPJL antibody	>5 publications with PTF1A and RBPJL
ISL1 antibody	>4 publications with PTF1A and ISL1
PAX4 antibody	>4 publications with PTF1A and PAX4
FGF10 antibody	>4 publications with PTF1A and FGF10

Pathways associated with anti-PTF1A antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram
Regulation Of Beta-cell Development Pathway antibodies	Regulation Of Beta-cell Development Pathway Diagram
Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway antibodies	Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway Diagram

Diseases associated with anti-PTF1A antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>10 publications with PTF1A and Neoplasms
Congenital Abnormalities Antibodies	>9 publications with PTF1A and Congenital Abnormalities
Disease Models, Animal Antibodies	>8 publications with PTF1A and Disease Models, Animal
Heart Defects, Congenital Antibodies	>4 publications with PTF1A and Heart Defects, Congenital
Atrophy Antibodies	>2 publications with PTF1A and Atrophy
Inflammation Antibodies	>2 publications with PTF1A and Inflammation
Cell Transformation, Neoplastic Antibodies	>2 publications with PTF1A and Cell Transformation, Neoplastic
Skin Diseases Antibodies	>2 publications with PTF1A and Skin Diseases
Hydrocephalus Antibodies	>1 publications with PTF1A and Hydrocephalus
Learning Disorders Antibodies	>1 publications with PTF1A and Learning Disorders

Organs/Tissues associated with anti-PTF1A antibody

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>99 publications with PTF1A and Pancreas